Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients

ABSTRACT: Background: CD4+/CD8+ double positive (DP) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases. In chronic viral infections, this cell subset has effector memory phenotype and displays antigen specificity. No previous studies of double positive T cells in parasite infections have been carried out. Methodology/Principal Findings: Seventeen chronic chagasic patients (7 asymptomatic and 10 symptomatic) and 24 noninfected donors, including 12 healthy and 12 with non-chagasic cardiomyopathy donors were analyzed. Peripheral blood was stained for CD3, CD4, CD8, HLA-DR and CD38, and lymphocytes for intracellular perforin. Antigen specificity was assessed using HLA*A2 tetramers loaded with T. cruzi K1 or influenza virus epitopes. Surface expression of CD107 and intracellular IFN-c production were determined in K1-specific DP T cells from 11 chagasic donors. Heart tissue from a chronic chagasic patient was stained for both CD8 and CD4 by immunochemistry. Chagasic patients showed higher frequencies of DP T cells (2.1%60.9) compared with healthy (1.1%60.5) and non-chagasic cardiomyopathy (1.2%60.4) donors. DP T cells from Chagasic patients also expressed more HLA-DR, CD38 and perforin and had higher frequencies of T. cruzi K1-specific cells. IFN-c production in K1-specific cells was higher in asymptomatic patients after polyclonal stimulation, while these cells tended to degranulate more in symptomatic donors. Immunochemistry revealed that double positive T cells infiltrate the cardiac tissue of a chagasic donor. Conclusions: Chagasic patients have higher percentages of circulating double positive T cells expressing activation markers, potential effector molecules and greater class I antigenic specificity against T. cruzi. Although K1 tetramer positive DP T cell produced little IFN-c, they displayed degranulation activity that was increased in symptomatic patients. Moreover, K1- specific DP T cells can migrate to the heart tissue.

• Autores: Bedoya, Astrid Milena; Olaya, Natalia; Giraldo, Nicolás A.; Bolaños, Natalia I.; Cuellar Ávila, Adriana; Guzmán, Fanny; Uribe, Ana María; Cucunuba, Zulma M.; Roa, Nubia; Rosas, Fernando; Velasco, Víctor; Puerta, Concepción J.; González, John M.
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2011
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39557
• Acceso en línea: https://hdl.handle.net/10495/39557
• Palabra clave: Anciano de 80 o más Años; Aged, 80 and over; Antígenos CD; Antigens, CD; Linfocitos T CD4-Positivos; CD4-Positive T-Lymphocytes; Cardiomiopatía Chagásica; Chagas Cardiomyopathy; Antígenos HLA; HLA Antigens; Interferón gamma; Interferon-gamma; Miocardio - patología; Myocardium - pathology; Perforina - análisis; Perforin - analysis; Subgrupos de Linfocitos T; T-Lymphocyte Subsets; https://id.nlm.nih.gov/mesh/D000369; https://id.nlm.nih.gov/mesh/D015703; https://id.nlm.nih.gov/mesh/D015496; https://id.nlm.nih.gov/mesh/D002598; https://id.nlm.nih.gov/mesh/D006680; https://id.nlm.nih.gov/mesh/D007371; https://id.nlm.nih.gov/mesh/D009206; https://id.nlm.nih.gov/mesh/D054353; https://id.nlm.nih.gov/mesh/D016176
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/