Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis
ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the reg...
- Autores:
-
Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/20379
- Acceso en línea:
- http://hdl.handle.net/10495/20379
- Palabra clave:
- Antioxidantes
Antioxidants
Histología
Histology
Plant Somatic Embryogenesis Techniques
Técnicas de Embriogénesis Somática de Plantas
Banco de semillas del suelo
Soil seed banks
Embriogénesis somática
Somatic embryogenesis
Recalcitrancia
http://aims.fao.org/aos/agrovoc/c_35298
http://aims.fao.org/aos/agrovoc/c_36911
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| dc.title.translated.spa.fl_str_mv |
Análisis de distribución de polifenoles y sustancias de reserva en embriogénesis somática de cacao |
| title |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| spellingShingle |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis Antioxidantes Antioxidants Histología Histology Plant Somatic Embryogenesis Techniques Técnicas de Embriogénesis Somática de Plantas Banco de semillas del suelo Soil seed banks Embriogénesis somática Somatic embryogenesis Recalcitrancia http://aims.fao.org/aos/agrovoc/c_35298 http://aims.fao.org/aos/agrovoc/c_36911 |
| title_short |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| title_full |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| title_fullStr |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| title_full_unstemmed |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| title_sort |
Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesis |
| dc.creator.fl_str_mv |
Gallego Rúa, Adriana María Henao Ramírez, Ana María Urrea Trujillo, Aura Inés Atehortúa Garcés, Lucía |
| dc.contributor.author.none.fl_str_mv |
Gallego Rúa, Adriana María Henao Ramírez, Ana María Urrea Trujillo, Aura Inés Atehortúa Garcés, Lucía |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biotecnología |
| dc.subject.decs.none.fl_str_mv |
Antioxidantes Antioxidants Histología Histology Plant Somatic Embryogenesis Techniques Técnicas de Embriogénesis Somática de Plantas |
| topic |
Antioxidantes Antioxidants Histología Histology Plant Somatic Embryogenesis Techniques Técnicas de Embriogénesis Somática de Plantas Banco de semillas del suelo Soil seed banks Embriogénesis somática Somatic embryogenesis Recalcitrancia http://aims.fao.org/aos/agrovoc/c_35298 http://aims.fao.org/aos/agrovoc/c_36911 |
| dc.subject.agrovoc.none.fl_str_mv |
Banco de semillas del suelo Soil seed banks Embriogénesis somática Somatic embryogenesis |
| dc.subject.proposal.spa.fl_str_mv |
Recalcitrancia |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_35298 http://aims.fao.org/aos/agrovoc/c_36911 |
| description |
ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2021-06-25T13:11:53Z |
| dc.date.available.none.fl_str_mv |
2021-06-25T13:11:53Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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: Gallego Rúa AM, Henao Ramírez AM, Urrea Trujillo AI, Atehortúa Garcés L. Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta biol. Colomb. 2016;21(2):335-345. doi: http://dx.doi.org/10.15446/abc.v21n2.50196 |
| dc.identifier.issn.none.fl_str_mv |
0120-548X |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/20379 |
| dc.identifier.doi.none.fl_str_mv |
10.15446/abc.v21n2.50196 |
| dc.identifier.eissn.none.fl_str_mv |
1900-1649 |
| identifier_str_mv |
: Gallego Rúa AM, Henao Ramírez AM, Urrea Trujillo AI, Atehortúa Garcés L. Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta biol. Colomb. 2016;21(2):335-345. doi: http://dx.doi.org/10.15446/abc.v21n2.50196 0120-548X 10.15446/abc.v21n2.50196 1900-1649 |
| url |
http://hdl.handle.net/10495/20379 |
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eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Acta biol. Colomb. |
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345 |
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2 |
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335 |
| dc.relation.citationvolume.spa.fl_str_mv |
21 |
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Acta Biológica Colombiana |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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12 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología |
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Bogotá, Colombia |
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Universidad de Antioquia |
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Gallego Rúa, Adriana MaríaHenao Ramírez, Ana MaríaUrrea Trujillo, Aura InésAtehortúa Garcés, LucíaBiotecnología2021-06-25T13:11:53Z2021-06-25T13:11:53Z2016: Gallego Rúa AM, Henao Ramírez AM, Urrea Trujillo AI, Atehortúa Garcés L. Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta biol. Colomb. 2016;21(2):335-345. doi: http://dx.doi.org/10.15446/abc.v21n2.501960120-548Xhttp://hdl.handle.net/10495/2037910.15446/abc.v21n2.501961900-1649ABSTRACT: In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step.RESUMEN: Dos variedades de cacao con diferentes respuestas a la regeneración fueron descritas en función de su capacidad para almacenar diferentes compuestos, con el fin de aproximarse al entendimiento de las causas de la falta de regeneración en embriones somáticos de cacao. Es bien sabido que las reservas de semillas desempeñan un papel central en la capacidad de regeneración de embriones somáticos; por tanto, se realizó un seguimiento de cambios histoquímicos y fluctuaciones de reserva de proteínas, polisacáridos y polifenoles durante la embriogénesis somática (SE) en dos variedades de cacao. El estudio mostró que, en los embriones somáticos de la variedad regenerante, los polifenoles se localizaron principalmente en la periferia del embrión (células de la epidermis) y las proteínas fueron el componente principal de almacenamiento en el medio de expresión de embriones, mientras que la variedad no regenerante tenía una alta presencia de polisacáridos y una distribución aleatoria de los polifenoles en el final de la etapa de inducción de embriones.COL000108412application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaBogotá, Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Polyphenols distribution and reserve substances analysis in cocoa somatic embryogenesisAnálisis de distribución de polifenoles y sustancias de reserva en embriogénesis somática de cacaoArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAntioxidantesAntioxidantsHistologíaHistologyPlant Somatic Embryogenesis TechniquesTécnicas de Embriogénesis Somática de PlantasBanco de semillas del sueloSoil seed banksEmbriogénesis somáticaSomatic embryogenesisRecalcitranciahttp://aims.fao.org/aos/agrovoc/c_35298http://aims.fao.org/aos/agrovoc/c_36911Acta biol. 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