Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies

ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. pana- mensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm2, 15 min), twice a week for 3 weeks.

• Autores: Montoya Cuervo, Edwin Andrés; Daza Figueredo, Juan Alejandro; Muñoz Herrera, Diana Lorena; Ríos Ramírez, Yesmit Karina; Taylor Orozco, Viviana Milena; Cedeño, David; Vélez Bernal, Iván Darío; Echeverri López, Luis Fernando; Robledo Restrepo, Sara María
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2015
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/32966
• Acceso en línea: https://hdl.handle.net/10495/32966
• Palabra clave: Antracenos; Anthracenes; Línea Celular; Cell Line; Cricetinae; Leishmania; Leishmaniasis Cutánea; Leishmaniasis, Cutaneous; Perileno; Perylene; Fotoquimioterapia; Photochemotherapy; Fármacos Fotosensibilizantes; Photosensitizing Agents; Células U937; U937 Cells; Cicatrización de Heridas; Wound Healing
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/2.5/co/