Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes
ABSTRACT: Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods:The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expres...
- Autores:
-
Toro Londoño, Miguel Ángel
Patiño González, Edwin Bairon
Sara María, Robledo Restrepo
Jiménez Ruíz, Antonio
Alzate Restrepo, Juan Fernando
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2011
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/22209
- Acceso en línea:
- http://hdl.handle.net/10495/22209
- Palabra clave:
- Proteínas Recombinantes
Recombinant Proteins
Filogenia
Phylogeny
Leishmania panamensis
Endonucleasas
Endonucleasa
Caracterización molecular
Molecular characterization
Endonuclease G
Renaturalización
http://aims.fao.org/aos/agrovoc/c_cfd74cdc
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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|
| dc.title.spa.fl_str_mv |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| dc.title.translated.spa.fl_str_mv |
Leishmania (Viannia) panamensis expresa una nucleasa molecular y bioquímicamente similar a la Endonucleasa G de eucariotas superiores |
| title |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| spellingShingle |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes Proteínas Recombinantes Recombinant Proteins Filogenia Phylogeny Leishmania panamensis Endonucleasas Endonucleasa Caracterización molecular Molecular characterization Endonuclease G Renaturalización http://aims.fao.org/aos/agrovoc/c_cfd74cdc |
| title_short |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| title_full |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| title_fullStr |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| title_full_unstemmed |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| title_sort |
Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes |
| dc.creator.fl_str_mv |
Toro Londoño, Miguel Ángel Patiño González, Edwin Bairon Sara María, Robledo Restrepo Jiménez Ruíz, Antonio Alzate Restrepo, Juan Fernando |
| dc.contributor.author.none.fl_str_mv |
Toro Londoño, Miguel Ángel Patiño González, Edwin Bairon Sara María, Robledo Restrepo Jiménez Ruíz, Antonio Alzate Restrepo, Juan Fernando |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Bioquímica Estructural de Macromoléculas Grupo de Parasitología Universidad de Antioquia Programa de Estudio y Control de Enfermedades Tropicales (PECET) |
| dc.subject.decs.none.fl_str_mv |
Proteínas Recombinantes Recombinant Proteins Filogenia Phylogeny Leishmania panamensis Endonucleasas Endonucleasa |
| topic |
Proteínas Recombinantes Recombinant Proteins Filogenia Phylogeny Leishmania panamensis Endonucleasas Endonucleasa Caracterización molecular Molecular characterization Endonuclease G Renaturalización http://aims.fao.org/aos/agrovoc/c_cfd74cdc |
| dc.subject.agrovoc.none.fl_str_mv |
Caracterización molecular Molecular characterization |
| dc.subject.proposal.spa.fl_str_mv |
Endonuclease G Renaturalización |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_cfd74cdc |
| description |
ABSTRACT: Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods:The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion,temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range. |
| publishDate |
2011 |
| dc.date.issued.none.fl_str_mv |
2011 |
| dc.date.accessioned.none.fl_str_mv |
2021-09-04T16:06:33Z |
| dc.date.available.none.fl_str_mv |
2021-09-04T16:06:33Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Toro, M., Patiño, E., Robledo, S., Jiménez, A., & Alzate, J. (2011). Leishmania (Viannia) panamensis expresa una nucleasa con características moleculares y bioquímicas similares a la endonucleasa G de eucariotas superiores *. Colombia Médica , 42 (2), 154-165. https://doi.org/10.25100/cm.v42i2.766 |
| dc.identifier.issn.none.fl_str_mv |
0120-8322 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/22209 |
| dc.identifier.doi.none.fl_str_mv |
10.25100/cm.v42i2.766 |
| dc.identifier.eissn.none.fl_str_mv |
1657-9534 |
| identifier_str_mv |
Toro, M., Patiño, E., Robledo, S., Jiménez, A., & Alzate, J. (2011). Leishmania (Viannia) panamensis expresa una nucleasa con características moleculares y bioquímicas similares a la endonucleasa G de eucariotas superiores *. Colombia Médica , 42 (2), 154-165. https://doi.org/10.25100/cm.v42i2.766 0120-8322 10.25100/cm.v42i2.766 1657-9534 |
| url |
http://hdl.handle.net/10495/22209 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Colomb. med. |
| dc.relation.citationendpage.spa.fl_str_mv |
165 |
| dc.relation.citationissue.spa.fl_str_mv |
2 |
| dc.relation.citationstartpage.spa.fl_str_mv |
154 |
| dc.relation.citationvolume.spa.fl_str_mv |
42 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Colombia Médica |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ https://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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12 |
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application/pdf |
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Universidad del Valle |
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Cali, Colombia |
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Universidad de Antioquia |
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Toro Londoño, Miguel ÁngelPatiño González, Edwin BaironSara María, Robledo RestrepoJiménez Ruíz, AntonioAlzate Restrepo, Juan FernandoGrupo de Bioquímica Estructural de MacromoléculasGrupo de Parasitología Universidad de AntioquiaPrograma de Estudio y Control de Enfermedades Tropicales (PECET)2021-09-04T16:06:33Z2021-09-04T16:06:33Z2011Toro, M., Patiño, E., Robledo, S., Jiménez, A., & Alzate, J. (2011). Leishmania (Viannia) panamensis expresa una nucleasa con características moleculares y bioquímicas similares a la endonucleasa G de eucariotas superiores *. Colombia Médica , 42 (2), 154-165. https://doi.org/10.25100/cm.v42i2.7660120-8322http://hdl.handle.net/10495/2220910.25100/cm.v42i2.7661657-9534ABSTRACT: Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods:The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion,temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range.RESUMEN: Objetivo: Caracterizar molecular y bioquímicamente la Endonucleasa G (EndoG) de Leishmania (Viannia) panamensis. Métodos: El gen de la putativa Endonucleasa G de L. (V.) panamensis fue amplificado, clonado y secuenciado. La proteína recombinante se produjo en un sistema de expresión heterólogo y la proteína activa se sometió a pruebas bioquímicas para determinar la preferencia de iones, temperatura y pH. Resultados: L a rENDOG de L. (V.) panamensis muestra características bioquímicas similares a aquellas descritas en otros trypanosomatidos y en eucariotas superiores. Además, los análisis filogenéticos muestran una posible relación evolutiva con la Endonucleasa G de metazoos. Conclusiones: Leishmania (V.) panamensis posee un gen que codifica para una endonucleasa homóloga a la ENDOG deotros organismos superiores, que se puede producir de forma recombinante en Escherichia coli y que es capaz de degradar ADN circular cerrado de doble cadena. Tiene una preferencia por los iones magnesio y manganeso para usarlos como cofactor y es inhibida por el potasio. Además, funciona en un amplio rango de pH y temperatura.COL0015099COL0007506COL015627512application/pdfengUniversidad del ValleCali, Colombiahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotesLeishmania (Viannia) panamensis expresa una nucleasa molecular y bioquímicamente similar a la Endonucleasa G de eucariotas superioresArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionProteínas RecombinantesRecombinant ProteinsFilogeniaPhylogenyLeishmania panamensisEndonucleasasEndonucleasaCaracterización molecularMolecular characterizationEndonuclease GRenaturalizaciónhttp://aims.fao.org/aos/agrovoc/c_cfd74cdcColomb. med.165215442Colombia MédicaPublicationORIGINALToroMiguel_2011_LeishmaniaEndonucleaseG.pdfToroMiguel_2011_LeishmaniaEndonucleaseG.pdfArtículo de investigaciónapplication/pdf1968505https://bibliotecadigital.udea.edu.co/bitstreams/11fb8104-9689-4b4b-acec-2280e0a5b0ce/download218649032921b67b5da0bca84734f0c8MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstreams/ef8aa9ae-f086-4fb6-918e-c9a78e12c1d3/downloadb88b088d9957e670ce3b3fbe2eedbc13MD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/bb69934c-f31e-4c97-afc5-4f7f113c5520/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTToroMiguel_2011_LeishmaniaEndonucleaseG.pdf.txtToroMiguel_2011_LeishmaniaEndonucleaseG.pdf.txtExtracted texttext/plain42016https://bibliotecadigital.udea.edu.co/bitstreams/03c40f21-ca08-456f-add9-4a5f8df4f25f/download1b5bd080908f9a68d4dc022bb16190cfMD54falseAnonymousREADTHUMBNAILToroMiguel_2011_LeishmaniaEndonucleaseG.pdf.jpgToroMiguel_2011_LeishmaniaEndonucleaseG.pdf.jpgGenerated Thumbnailimage/jpeg15342https://bibliotecadigital.udea.edu.co/bitstreams/8ba6f9ad-51f5-430d-8f67-958e5b67ca9e/download525eaa047dc2c7fb60e7b0edee17cff2MD55falseAnonymousREAD10495/22209oai:bibliotecadigital.udea.edu.co:10495/222092025-03-27 01:44:02.868http://creativecommons.org/licenses/by-nc-nd/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |