Leishmania (Viannia) panamensis expresses a nuclease with molecular andbiochemical features similar to the Endonuclease G of higher eukaryotes

ABSTRACT: Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods:The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion,temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range.

• Autores: Toro Londoño, Miguel Ángel; Patiño González, Edwin Bairon; Sara María, Robledo Restrepo; Jiménez Ruíz, Antonio; Alzate Restrepo, Juan Fernando
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2011
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/22209
• Acceso en línea: http://hdl.handle.net/10495/22209
• Palabra clave: Proteínas Recombinantes; Recombinant Proteins; Filogenia; Phylogeny; Leishmania panamensis; Endonucleasas; Endonucleasa; Caracterización molecular; Molecular characterization; Endonuclease G; Renaturalización; http://aims.fao.org/aos/agrovoc/c_cfd74cdc
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/2.5/co/