Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC

Durante la gestación humana las vesículas extracelulares (VEs) producidas por la placenta participan en la comunicación materno-fetal, representando un reto para comprender su rol fisiológico y/o fisiopatológico en el embarazo. Aunque se ha evidenciado que las VEs contienen glicanos en su superficie...

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Autores:: Buitrago Orozco, Lizeth Catalina

Tipo de recurso:

Fecha de publicación:: 2025

Institución:: Universidad de Antioquia

Repositorio:: Repositorio UdeA

Idioma:: spa

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network_acronym_str	UDEA2
network_name_str	Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
title	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
spellingShingle	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC Vesículas extracelulares Extracellular vesicles Trofoblastos Trophoblasts Polisacáridos Polysaccharides Ácido N-acetilneuramínico N-acetylneuraminic acid Fucosa Fucose Endotelio Endothelium Células endoteliales de la vena umbilical humana Human umbilical vein endothelial cells Embarazo Pregnancy https://id.nlm.nih.gov/mesh/D000067128 https://id.nlm.nih.gov/mesh/D014327 https://id.nlm.nih.gov/mesh/D011134 https://id.nlm.nih.gov/mesh/D019158 https://id.nlm.nih.gov/mesh/D005643 https://id.nlm.nih.gov/mesh/D004727 https://id.nlm.nih.gov/mesh/D061307 https://id.nlm.nih.gov/mesh/D011247 ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
title_short	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
title_full	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
title_fullStr	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
title_full_unstemmed	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
title_sort	Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC
dc.creator.fl_str_mv	Buitrago Orozco, Lizeth Catalina
dc.contributor.advisor.none.fl_str_mv	Bueno Sánchez, Julio César Quintana Castillo, Juan Carlos
dc.contributor.author.none.fl_str_mv	Buitrago Orozco, Lizeth Catalina
dc.contributor.researchgroup.none.fl_str_mv	Grupo Reproducción
dc.contributor.jury.none.fl_str_mv	Pérez Cardona, David José Estrada Mira, Sergio
dc.subject.decs.none.fl_str_mv	Vesículas extracelulares Extracellular vesicles Trofoblastos Trophoblasts Polisacáridos Polysaccharides Ácido N-acetilneuramínico N-acetylneuraminic acid Fucosa Fucose Endotelio Endothelium Células endoteliales de la vena umbilical humana Human umbilical vein endothelial cells Embarazo Pregnancy
topic	Vesículas extracelulares Extracellular vesicles Trofoblastos Trophoblasts Polisacáridos Polysaccharides Ácido N-acetilneuramínico N-acetylneuraminic acid Fucosa Fucose Endotelio Endothelium Células endoteliales de la vena umbilical humana Human umbilical vein endothelial cells Embarazo Pregnancy https://id.nlm.nih.gov/mesh/D000067128 https://id.nlm.nih.gov/mesh/D014327 https://id.nlm.nih.gov/mesh/D011134 https://id.nlm.nih.gov/mesh/D019158 https://id.nlm.nih.gov/mesh/D005643 https://id.nlm.nih.gov/mesh/D004727 https://id.nlm.nih.gov/mesh/D061307 https://id.nlm.nih.gov/mesh/D011247 ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
dc.subject.meshuri.none.fl_str_mv	https://id.nlm.nih.gov/mesh/D000067128 https://id.nlm.nih.gov/mesh/D014327 https://id.nlm.nih.gov/mesh/D011134 https://id.nlm.nih.gov/mesh/D019158 https://id.nlm.nih.gov/mesh/D005643 https://id.nlm.nih.gov/mesh/D004727 https://id.nlm.nih.gov/mesh/D061307 https://id.nlm.nih.gov/mesh/D011247
dc.subject.ods.none.fl_str_mv	ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
description	Durante la gestación humana las vesículas extracelulares (VEs) producidas por la placenta participan en la comunicación materno-fetal, representando un reto para comprender su rol fisiológico y/o fisiopatológico en el embarazo. Aunque se ha evidenciado que las VEs contienen glicanos en su superficie y que estos participan en su captación por células blanco, aún no son claros los mecanismos de participación de los glicanos en la internalización de VEs y existe poca información sobre el perfil de glicanos presente en la superficie de VEs trofoblásticas. En esta investigación se aislaron y caracterizaron VEs trofoblásticas medianas y grandes, mediante histocultivos de vellosidades placentarias y encontramos que estas contienen los glicanos terminales α-manosa, β-galactosa, α-fucosa y ácido siálico α2-3 y α2-6. Particularmente, se evidenció una asociación entre el ácido siálico α2-3 y la carga superficial negativa, sugiriendo un posible efecto electrostático en las interacciones célula-vesícula. Posteriormente, se evaluó el efecto del ácido sia α2-3 y α-fucosa en la captación y la permeabilidad endotelial en células HUVEC, previamente tratadas con TNF-α para inducir la producción de moléculas de adhesión y sometidas a bloqueo competitivo con ácido siálico o fucosa. Encontramos que las VEs con alto contenido de sia α2-3 presentan menor captación, posiblemente por repulsión electrostática entre el sia α2-3 y el glicocálix endotelial. En conjunto, estos hallazgos aportan evidencia sobre el papel potencial de los glicanos terminales de VEs trofoblásticas en su interacción con células endoteliales, resaltando tanto su valor como marcadores estructurales como su contribución funcional sobre el endotelio materno.
publishDate	2025
dc.date.accessioned.none.fl_str_mv	2025-11-19T19:52:50Z
dc.date.issued.none.fl_str_mv	2025
dc.date.available.none.fl_str_mv	2027-11-19
dc.type.none.fl_str_mv	Trabajo de grado - Maestría
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/TM
dc.type.content.none.fl_str_mv	Text
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_b1a7d7d4d402bcce
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/draft
status_str	draft
dc.identifier.citation.none.fl_str_mv	Buitrago Orozco L. Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC [Tesis de maestría]. Medellín, Colombia. Universidad de Antioquia; 2025.
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/10495/48274
identifier_str_mv	Buitrago Orozco L. Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC [Tesis de maestría]. Medellín, Colombia. Universidad de Antioquia; 2025.
url	https://hdl.handle.net/10495/48274
dc.language.iso.none.fl_str_mv	spa
language	spa
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dc.format.extent.none.fl_str_mv	61 páginas
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dc.publisher.none.fl_str_mv	Universidad de Antioquia
dc.publisher.program.none.fl_str_mv	Maestría en Ciencias Básicas Biomédicas
dc.publisher.department.none.fl_str_mv	Departamento de Ciencias Básicas
dc.publisher.place.none.fl_str_mv	Medellín, Colombia
dc.publisher.faculty.none.fl_str_mv	Corporación Académica Ciencias Básicas Biomédicas
dc.publisher.branch.none.fl_str_mv	Campus en el Área de la salud
publisher.none.fl_str_mv	Universidad de Antioquia
institution	Universidad de Antioquia
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spelling	Bueno Sánchez, Julio CésarQuintana Castillo, Juan CarlosBuitrago Orozco, Lizeth CatalinaGrupo ReproducciónPérez Cardona, David JoséEstrada Mira, Sergio2025-11-19T19:52:50Z2027-11-192025Buitrago Orozco L. Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC [Tesis de maestría]. Medellín, Colombia. Universidad de Antioquia; 2025.https://hdl.handle.net/10495/48274Durante la gestación humana las vesículas extracelulares (VEs) producidas por la placenta participan en la comunicación materno-fetal, representando un reto para comprender su rol fisiológico y/o fisiopatológico en el embarazo. Aunque se ha evidenciado que las VEs contienen glicanos en su superficie y que estos participan en su captación por células blanco, aún no son claros los mecanismos de participación de los glicanos en la internalización de VEs y existe poca información sobre el perfil de glicanos presente en la superficie de VEs trofoblásticas. En esta investigación se aislaron y caracterizaron VEs trofoblásticas medianas y grandes, mediante histocultivos de vellosidades placentarias y encontramos que estas contienen los glicanos terminales α-manosa, β-galactosa, α-fucosa y ácido siálico α2-3 y α2-6. Particularmente, se evidenció una asociación entre el ácido siálico α2-3 y la carga superficial negativa, sugiriendo un posible efecto electrostático en las interacciones célula-vesícula. Posteriormente, se evaluó el efecto del ácido sia α2-3 y α-fucosa en la captación y la permeabilidad endotelial en células HUVEC, previamente tratadas con TNF-α para inducir la producción de moléculas de adhesión y sometidas a bloqueo competitivo con ácido siálico o fucosa. Encontramos que las VEs con alto contenido de sia α2-3 presentan menor captación, posiblemente por repulsión electrostática entre el sia α2-3 y el glicocálix endotelial. En conjunto, estos hallazgos aportan evidencia sobre el papel potencial de los glicanos terminales de VEs trofoblásticas en su interacción con células endoteliales, resaltando tanto su valor como marcadores estructurales como su contribución funcional sobre el endotelio materno.During human pregnancy, extracellular vesicles (EVs) produced by the placenta participate in maternal–fetal communication, posing a challenge for understanding their physiological and/or pathophysiological roles in gestation. Although EVs have been shown to contain surface glycans that participate in their uptake by target cells, the mechanisms by which glycans contribute to EV internalization remain unclear, and little information is available regarding the glycan profile present on the surface of trophoblastic EVs. In this study, medium and large trophoblastic EVs were isolated and characterized from placental villous explants, and we found that they contain the terminal glycans α-mannose, β-galactose, α-fucose, and sialic acid α2-3 and α2-6. Notably, an association was observed between α2-3-linked sialic acid and negative surface charge, suggesting a possible electrostatic effect in cell–vesicle interactions. Subsequently, the effect of α2-3-linked sialic acid and α-fucose on EV uptake and endothelial permeability was evaluated in HUVEC cells previously treated with TNF-α to induce the expression of adhesion molecules and subjected to competitive blocking with sialic acid or fucose. We found that EVs with a high content of α2-3 sialic acid exhibited reduced uptake, possibly due to electrostatic repulsion between α2-3 sialic acid and the endothelial glycocalyx. Taken together, these findings provide evidence for the potential role of terminal glycans in trophoblastic EVs in their interaction with endothelial cells, highlighting both their value as structural markers and their functional contribution to the maternal endothelium.GlicobiologíaCOL0007631MaestríaMagíster en Ciencias Básicas Biomédicas61 páginasapplication/pdfspaUniversidad de AntioquiaMaestría en Ciencias Básicas BiomédicasDepartamento de Ciencias BásicasMedellín, ColombiaCorporación Académica Ciencias Básicas BiomédicasCampus en el Área de la saludhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-ShareAlike 4.0 Internationalhttp://purl.org/coar/access_right/c_abf2Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVECTrabajo de grado - Maestríahttp://purl.org/redcol/resource_type/TMTexthttp://purl.org/coar/version/c_b1a7d7d4d402bcceinfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/draftNoyola-Martínez N, Halhali A, Barrera D. 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

Caracterización de glicanos terminales asociados a vesículas extracelulares de placentas y sus efectos funcionales en células HUVEC

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