Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki
ABSTRACT: The Hepatitis C Virus (HCV) encodes the structural protein Core, which in addition to being the capsid subunit, participates in different mechanisms of HCV infection pathogenesis. Since HCV in vitro replication system has limitations, the use of viral vectors could be a useful tool to stud...
- Autores:
-
Mira González, Catalina
Yepes Correa, Jesús Orlando
Henao Castrillón, Luis Felipe
Montoya Guzmán, Melissa
Navas Navas, María Cristina
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/40157
- Acceso en línea:
- https://hdl.handle.net/10495/40157
- Palabra clave:
- Genes p53
Genes, p53
Hepacivirus
Proteínas del Núcleo Viral
Viral Core Proteins
Infecciones por Alphavirus
Alphavirus Infections
Células Hep G2
Hep G2 Cells
https://id.nlm.nih.gov/mesh/D016158
https://id.nlm.nih.gov/mesh/D016174
https://id.nlm.nih.gov/mesh/D014758
https://id.nlm.nih.gov/mesh/D018354
https://id.nlm.nih.gov/mesh/D056945
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| dc.title.translated.spa.fl_str_mv |
Hepatitis C virus core protein expression in HepG2 cells using semliki forest virus |
| title |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| spellingShingle |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki Genes p53 Genes, p53 Hepacivirus Proteínas del Núcleo Viral Viral Core Proteins Infecciones por Alphavirus Alphavirus Infections Células Hep G2 Hep G2 Cells https://id.nlm.nih.gov/mesh/D016158 https://id.nlm.nih.gov/mesh/D016174 https://id.nlm.nih.gov/mesh/D014758 https://id.nlm.nih.gov/mesh/D018354 https://id.nlm.nih.gov/mesh/D056945 |
| title_short |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| title_full |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| title_fullStr |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| title_full_unstemmed |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| title_sort |
Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de Semliki |
| dc.creator.fl_str_mv |
Mira González, Catalina Yepes Correa, Jesús Orlando Henao Castrillón, Luis Felipe Montoya Guzmán, Melissa Navas Navas, María Cristina |
| dc.contributor.author.none.fl_str_mv |
Mira González, Catalina Yepes Correa, Jesús Orlando Henao Castrillón, Luis Felipe Montoya Guzmán, Melissa Navas Navas, María Cristina |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Gastrohepatología |
| dc.subject.decs.none.fl_str_mv |
Genes p53 Genes, p53 Hepacivirus Proteínas del Núcleo Viral Viral Core Proteins Infecciones por Alphavirus Alphavirus Infections Células Hep G2 Hep G2 Cells |
| topic |
Genes p53 Genes, p53 Hepacivirus Proteínas del Núcleo Viral Viral Core Proteins Infecciones por Alphavirus Alphavirus Infections Células Hep G2 Hep G2 Cells https://id.nlm.nih.gov/mesh/D016158 https://id.nlm.nih.gov/mesh/D016174 https://id.nlm.nih.gov/mesh/D014758 https://id.nlm.nih.gov/mesh/D018354 https://id.nlm.nih.gov/mesh/D056945 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D016158 https://id.nlm.nih.gov/mesh/D016174 https://id.nlm.nih.gov/mesh/D014758 https://id.nlm.nih.gov/mesh/D018354 https://id.nlm.nih.gov/mesh/D056945 |
| description |
ABSTRACT: The Hepatitis C Virus (HCV) encodes the structural protein Core, which in addition to being the capsid subunit, participates in different mechanisms of HCV infection pathogenesis. Since HCV in vitro replication system has limitations, the use of viral vectors could be a useful tool to study the Core protein properties. To validate the Semliki Forest Virus (SFV) strategy in transduced HepG2 cells to study the HCV Core protein, the expression of green fluorescent protein (GFP) and Core protein expressions were detected 24 to 96 hours post-transduction in HepG2 cells transduced with rSFV. Core protein expression was lower than GFP expression in HepG2 cells. Since HCV Core protein can regulate the activity of the p53 gene, the transcriptional level of this gene was evaluated. A decrease in the level of p53 mRNA was observed in the cells after transduction, compared to the control cells. Although the cells transduced with rSFV-Core had the lowest level of p53 mRNA, the difference was not significant compared to cells transduced with rSFV-GFP. The results confirm that rSFV allows the transient expression of heterologous proteins in human hepatoma cell lines. Additional studies are needed to determine whether the decreased expression of Core may be due to the degradation of the viral protein. |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2024-06-19T23:20:06Z |
| dc.date.available.none.fl_str_mv |
2024-06-19T23:20:06Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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0120-548X |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/40157 |
| dc.identifier.doi.none.fl_str_mv |
10.15446/abc.v26n1.79365 |
| dc.identifier.eissn.none.fl_str_mv |
1900-1649 |
| identifier_str_mv |
0120-548X 10.15446/abc.v26n1.79365 1900-1649 |
| url |
https://hdl.handle.net/10495/40157 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Acta. Biolo. Colomb. |
| dc.relation.citationendpage.spa.fl_str_mv |
218 |
| dc.relation.citationissue.spa.fl_str_mv |
1 |
| dc.relation.citationstartpage.spa.fl_str_mv |
209 |
| dc.relation.citationvolume.spa.fl_str_mv |
26 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Acta Biológica Colombiana |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ https://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
| dc.format.extent.spa.fl_str_mv |
9 páginas |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología |
| dc.publisher.place.spa.fl_str_mv |
Bogotá, Colombia |
| institution |
Universidad de Antioquia |
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Mira González, CatalinaYepes Correa, Jesús OrlandoHenao Castrillón, Luis FelipeMontoya Guzmán, MelissaNavas Navas, María CristinaGrupo de Gastrohepatología2024-06-19T23:20:06Z2024-06-19T23:20:06Z20210120-548Xhttps://hdl.handle.net/10495/4015710.15446/abc.v26n1.793651900-1649ABSTRACT: The Hepatitis C Virus (HCV) encodes the structural protein Core, which in addition to being the capsid subunit, participates in different mechanisms of HCV infection pathogenesis. Since HCV in vitro replication system has limitations, the use of viral vectors could be a useful tool to study the Core protein properties. To validate the Semliki Forest Virus (SFV) strategy in transduced HepG2 cells to study the HCV Core protein, the expression of green fluorescent protein (GFP) and Core protein expressions were detected 24 to 96 hours post-transduction in HepG2 cells transduced with rSFV. Core protein expression was lower than GFP expression in HepG2 cells. Since HCV Core protein can regulate the activity of the p53 gene, the transcriptional level of this gene was evaluated. A decrease in the level of p53 mRNA was observed in the cells after transduction, compared to the control cells. Although the cells transduced with rSFV-Core had the lowest level of p53 mRNA, the difference was not significant compared to cells transduced with rSFV-GFP. The results confirm that rSFV allows the transient expression of heterologous proteins in human hepatoma cell lines. Additional studies are needed to determine whether the decreased expression of Core may be due to the degradation of the viral protein.RESUMEN: El Virus de la Hepatitis C (VHC) codifica la proteína Core. Que, además de ser la subunidad de la cápside, participa en diferentes mecanismos de patogénesis de la infección por VHC. Dado que el sistema de replicación in vitro del VHC presenta limitaciones, el uso de vectores virales podría ser una herramienta útil para estudiar las propiedades de la proteína Core. Con el fin de validar el vector con el Virus del Bosque de Semliki (SFV) para el estudio de Core en células HepG2, se evaluó la expresión de la proteína verde fluorescente (GFP) y la proteína Core utilizando este vector viral. Las expresiones de GFP y Core se detectaron en células HepG2 transducidas con rSFV de 24 a 96 horas postransducción. La expresión de la proteína Core fue inferior a la expresión de GFP en las células HepG2. Teniendo en cuenta que la proteína Core del VHC puede regular la actividad del gen p53, se evaluó el nivel transcripcional de este gen. Se observó una disminución en el nivel de mARN de p53 en las células luego de la transducción, comparado con las células control. Aunque las células transducidas con rSFV-Core presentaron el menor nivel de mARN de p53, la diferencia no fue significativa comparada con las células transducidas con rSFV-GFP. Los resultados confirman que rSFV permite la expresión transitoria de proteínas heterólogas en líneas celulares de hepatoma humano. Se necesitan estudios adicionales para determinar si la expresión disminuida de Core puede deberse a degradación de la proteína viral.Universidad de Antioquia. Vicerrectoría de investigación. Comité para el Desarrollo de la Investigación - CODIThe World Academy of SciencesCOL00241599 páginasapplication/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaBogotá, Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Expresión de la Proteína Core del Virus de la Hepatitis C en Células HEPG2 Usando el Virus del Bosque de SemlikiHepatitis C virus core protein expression in HepG2 cells using semliki forest virusArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionGenes p53Genes, p53HepacivirusProteínas del Núcleo ViralViral Core ProteinsInfecciones por AlphavirusAlphavirus InfectionsCélulas Hep G2Hep G2 Cellshttps://id.nlm.nih.gov/mesh/D016158https://id.nlm.nih.gov/mesh/D016174https://id.nlm.nih.gov/mesh/D014758https://id.nlm.nih.gov/mesh/D018354https://id.nlm.nih.gov/mesh/D056945Acta. Biolo. 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