In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine
ABSTRACT: Background: Coronavirus infectious disease 2019 (COVID-19) caused by the infection with the new coronavirus SARS-CoV-2 has affected the life and health of more than 222 million people. In the absence of any specific pharmacological treatment, the need to find new therapeutic alternatives i...
- Autores:
-
Flórez Álvarez, Lizdany
Martínez Moreno, Jahnnyer Anilio
Zapata Cardona, María Isabel
Galeano Jaramillo, Elkin de Jesús
Alzate Guarín, Fernando
Zapata Builes, Wildeman
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/39180
- Acceso en línea:
- https://hdl.handle.net/10495/39180
- Palabra clave:
- SARS-CoV-2
Extractos Vegetales
Plant Extracts
Antivirales
Antiviral Agents
Etnobotánica
Ethnobotany
https://id.nlm.nih.gov/mesh/D000086402
https://id.nlm.nih.gov/mesh/D010936
https://id.nlm.nih.gov/mesh/D000998
https://id.nlm.nih.gov/mesh/D019448
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| dc.title.translated.spa.fl_str_mv |
Actividad antiviral in vitro contra el SARS-CoV-2 de extractos de plantas usados en medicina tradicional colombiana |
| title |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| spellingShingle |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine SARS-CoV-2 Extractos Vegetales Plant Extracts Antivirales Antiviral Agents Etnobotánica Ethnobotany https://id.nlm.nih.gov/mesh/D000086402 https://id.nlm.nih.gov/mesh/D010936 https://id.nlm.nih.gov/mesh/D000998 https://id.nlm.nih.gov/mesh/D019448 |
| title_short |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| title_full |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| title_fullStr |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| title_full_unstemmed |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| title_sort |
In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine |
| dc.creator.fl_str_mv |
Flórez Álvarez, Lizdany Martínez Moreno, Jahnnyer Anilio Zapata Cardona, María Isabel Galeano Jaramillo, Elkin de Jesús Alzate Guarín, Fernando Zapata Builes, Wildeman |
| dc.contributor.author.none.fl_str_mv |
Flórez Álvarez, Lizdany Martínez Moreno, Jahnnyer Anilio Zapata Cardona, María Isabel Galeano Jaramillo, Elkin de Jesús Alzate Guarín, Fernando Zapata Builes, Wildeman |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Estudios Botánicos Grupo de Investigación en Sustancias Bioactivas (GISB) Inmunovirología Salud y Comunidad |
| dc.subject.decs.none.fl_str_mv |
SARS-CoV-2 Extractos Vegetales Plant Extracts Antivirales Antiviral Agents Etnobotánica Ethnobotany |
| topic |
SARS-CoV-2 Extractos Vegetales Plant Extracts Antivirales Antiviral Agents Etnobotánica Ethnobotany https://id.nlm.nih.gov/mesh/D000086402 https://id.nlm.nih.gov/mesh/D010936 https://id.nlm.nih.gov/mesh/D000998 https://id.nlm.nih.gov/mesh/D019448 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D000086402 https://id.nlm.nih.gov/mesh/D010936 https://id.nlm.nih.gov/mesh/D000998 https://id.nlm.nih.gov/mesh/D019448 |
| description |
ABSTRACT: Background: Coronavirus infectious disease 2019 (COVID-19) caused by the infection with the new coronavirus SARS-CoV-2 has affected the life and health of more than 222 million people. In the absence of any specific pharmacological treatment, the need to find new therapeutic alternatives is clear. Medicinal plants are widely used worldwide to treat different conditions, including COVID-19; however, in most cases, there are no specific studies to evaluate the efficacy of these treatments. Objective: This article evaluates the antiviral effect of six plant extracts used by indigenous and afro Colombian people against SARS-CoV-2 in vitro. Methods: The antiviral effect of six extracts prepared from plants used in Colombian traditional medicine was evaluated against SARS-CoV-2 through a pre-post treatment strategy on the Vero E6 cell line. Once cytotoxicity was established through an MTT assay, the antiviral effect of the extracts was calculated based on the reduction in the viral titer determined by plaque assay. Results: Gliricidia sepium inhibited SARS-CoV-2 in a 75.6%, 56.8%, 62.5% and 40.0% at 10 mg/mL, 8 mg/mL, 6 mg/mL, and 2 mg/mL, respectively, while Piper tuberculatum treatment reduced viral titer in 33.3% at 6 mg/mL after 48h. Conclusion: G. sepium and P. tuberculatum extracts exhibit antiviral activity against SARS-CoV-2 in vitro. |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-03T14:20:54Z |
| dc.date.available.none.fl_str_mv |
2024-05-03T14:20:54Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Flórez-Álvarez, L., Martínez-Moreno, J. ., Zapata-Cardona, M. I. ., Galeano, E. ., Alzate-Guarin, F. ., & Zapata, W. . (2022). In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine. Vitae, 29(1). https://doi.org/10.17533/udea.vitae.v29n1a347854 (Original work published February 6, 2022) |
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0121-4004 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/39180 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.vitae.v29n1a347854 |
| dc.identifier.eissn.none.fl_str_mv |
2145-2660 |
| identifier_str_mv |
Flórez-Álvarez, L., Martínez-Moreno, J. ., Zapata-Cardona, M. I. ., Galeano, E. ., Alzate-Guarin, F. ., & Zapata, W. . (2022). In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine. Vitae, 29(1). https://doi.org/10.17533/udea.vitae.v29n1a347854 (Original work published February 6, 2022) 0121-4004 10.17533/udea.vitae.v29n1a347854 2145-2660 |
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https://hdl.handle.net/10495/39180 |
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eng |
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eng |
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Vitae |
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11 |
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1 |
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1 |
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29 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Vitae |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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11 páginas |
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Universidad de Antioquia, Facultad de Ciencias Farmacéuticas y Alimentarias |
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Medellín, Colombia |
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Universidad de Antioquia |
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Flórez Álvarez, LizdanyMartínez Moreno, Jahnnyer AnilioZapata Cardona, María IsabelGaleano Jaramillo, Elkin de JesúsAlzate Guarín, FernandoZapata Builes, WildemanGrupo de Estudios BotánicosGrupo de Investigación en Sustancias Bioactivas (GISB)InmunovirologíaSalud y Comunidad2024-05-03T14:20:54Z2024-05-03T14:20:54Z2022Flórez-Álvarez, L., Martínez-Moreno, J. ., Zapata-Cardona, M. I. ., Galeano, E. ., Alzate-Guarin, F. ., & Zapata, W. . (2022). In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine. Vitae, 29(1). https://doi.org/10.17533/udea.vitae.v29n1a347854 (Original work published February 6, 2022)0121-4004https://hdl.handle.net/10495/3918010.17533/udea.vitae.v29n1a3478542145-2660ABSTRACT: Background: Coronavirus infectious disease 2019 (COVID-19) caused by the infection with the new coronavirus SARS-CoV-2 has affected the life and health of more than 222 million people. In the absence of any specific pharmacological treatment, the need to find new therapeutic alternatives is clear. Medicinal plants are widely used worldwide to treat different conditions, including COVID-19; however, in most cases, there are no specific studies to evaluate the efficacy of these treatments. Objective: This article evaluates the antiviral effect of six plant extracts used by indigenous and afro Colombian people against SARS-CoV-2 in vitro. Methods: The antiviral effect of six extracts prepared from plants used in Colombian traditional medicine was evaluated against SARS-CoV-2 through a pre-post treatment strategy on the Vero E6 cell line. Once cytotoxicity was established through an MTT assay, the antiviral effect of the extracts was calculated based on the reduction in the viral titer determined by plaque assay. Results: Gliricidia sepium inhibited SARS-CoV-2 in a 75.6%, 56.8%, 62.5% and 40.0% at 10 mg/mL, 8 mg/mL, 6 mg/mL, and 2 mg/mL, respectively, while Piper tuberculatum treatment reduced viral titer in 33.3% at 6 mg/mL after 48h. Conclusion: G. sepium and P. tuberculatum extracts exhibit antiviral activity against SARS-CoV-2 in vitro.RESUMEN: Introducción: La enfermedad infecciosa causada por el coronavirus 2019 (COVID-19) generada por la infección con el nuevo coronavirus SARS-CoV-2 ha afectado la vida y la salud de mas de 222 millones de personas. En ausencia de algún tratamiento farmacológico específico, la necesidad de encontrar nuevas alternativas terapéuticas es clara. Las plantas medicinales son utilizadas en todo el mundo para tratar diferentes condiciones, incluyendo el COVID-19; sin embargo, en la mayoría de los casos no existen estudios específicos que evalúen la eficacia de estos tratamientos. Objetivo: En este artículo, evaluamos el efecto antiviral de seis extractos de plantas usadas por pueblos indígenas y afrocolombianos contra el SARS-CoV-2 in vitro. Metodología: El efecto antiviral de seis extractos preparados a partir de plantas usadas en medicina tradicional colombiana fue evaluado contra SARS-CoV-2 por medio de una estrategia de pre-post tratamiento en células Vero E6. Una vez se estableció la citotoxicidad por un ensayo de MTT, el efecto antiviral de estos extractos fue calculado basado en la reducción del título viral determinado por ensayo de plaqueo. Resultados: G. sepium inhibió SARS-CoV-2 en un 75.6%, 56.8%, 62.5% y 40.0% a 10 mg/mL, 8 mg/mL, 6 mg/mL, and 2 mg/mL, respectivamente. Mientras el extracto de Piper tuberculatum redujo el título viral en un 33.3% a 6 mg/mL luego de 48h de tratamiento.Universidad de AntioquiaUniversidad Cooperativa de ColombiaCOL0010359COL0008227COL0012444COL004744911 páginasapplication/pdfengUniversidad de Antioquia, Facultad de Ciencias Farmacéuticas y AlimentariasMedellín, Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicineActividad antiviral in vitro contra el SARS-CoV-2 de extractos de plantas usados en medicina tradicional colombianaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionSARS-CoV-2Extractos VegetalesPlant ExtractsAntiviralesAntiviral AgentsEtnobotánicaEthnobotanyhttps://id.nlm.nih.gov/mesh/D000086402https://id.nlm.nih.gov/mesh/D010936https://id.nlm.nih.gov/mesh/D000998https://id.nlm.nih.gov/mesh/D019448Vitae111129VitaeBPIN 2020000100131- SGRRoR:03bp5hc83RoR:04td15k45PublicationORIGINALGaleanoElkin_2022_In_Vitro_Antiviral_Activity_SARS-CoV-2.pdfGaleanoElkin_2022_In_Vitro_Antiviral_Activity_SARS-CoV-2.pdfArtículo de investigaciónapplication/pdf563768https://bibliotecadigital.udea.edu.co/bitstreams/224de268-1f05-4a18-9e3d-746b75dfe544/downloadbbd6b144fef0ee46fddef8e85b0490c4MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; 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