In vitro antiviral activity against SARS-CoV-2 of plant extracts used in Colombian traditional medicine

ABSTRACT: Background: Coronavirus infectious disease 2019 (COVID-19) caused by the infection with the new coronavirus SARS-CoV-2 has affected the life and health of more than 222 million people. In the absence of any specific pharmacological treatment, the need to find new therapeutic alternatives is clear. Medicinal plants are widely used worldwide to treat different conditions, including COVID-19; however, in most cases, there are no specific studies to evaluate the efficacy of these treatments. Objective: This article evaluates the antiviral effect of six plant extracts used by indigenous and afro Colombian people against SARS-CoV-2 in vitro. Methods: The antiviral effect of six extracts prepared from plants used in Colombian traditional medicine was evaluated against SARS-CoV-2 through a pre-post treatment strategy on the Vero E6 cell line. Once cytotoxicity was established through an MTT assay, the antiviral effect of the extracts was calculated based on the reduction in the viral titer determined by plaque assay. Results: Gliricidia sepium inhibited SARS-CoV-2 in a 75.6%, 56.8%, 62.5% and 40.0% at 10 mg/mL, 8 mg/mL, 6 mg/mL, and 2 mg/mL, respectively, while Piper tuberculatum treatment reduced viral titer in 33.3% at 6 mg/mL after 48h. Conclusion: G. sepium and P. tuberculatum extracts exhibit antiviral activity against SARS-CoV-2 in vitro.

• Autores: Flórez Álvarez, Lizdany; Martínez Moreno, Jahnnyer Anilio; Zapata Cardona, María Isabel; Galeano Jaramillo, Elkin de Jesús; Alzate Guarín, Fernando; Zapata Builes, Wildeman
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39180
• Acceso en línea: https://hdl.handle.net/10495/39180
• Palabra clave: SARS-CoV-2; Extractos Vegetales; Plant Extracts; Antivirales; Antiviral Agents; Etnobotánica; Ethnobotany; https://id.nlm.nih.gov/mesh/D000086402; https://id.nlm.nih.gov/mesh/D010936; https://id.nlm.nih.gov/mesh/D000998; https://id.nlm.nih.gov/mesh/D019448
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/2.5/co/