PCR for equine infectious anemia virus detection in whole blood from naturally infected horses in Colombia

ABSTRACT: The equine infectious anemia virus is associated with a variety of clinical symptoms that include anorexia, weight loss, pyrexia, and anemia. Serological tests and reverse transcriptase nested polymerase chain reaction (RT-nPCR) have been used to detect EIAV in infected horses. In the present study, the use of non-nested PCR for the detection of ELAV gag gene sequences in peripheral blood cells DNA from horses bred in farms around Medellin (Colombia) is reported. EIAV DNA was detected by PCR in blood cells from fifteen horses, which had been found positive in an agar gel immunodiffusion test, despite the absence of clinical symptoms of expression. An amplified product of about 1.300 bp was obtained with a round of PCR without requiring nested PCR or RT-PCR.

• Autores: Trujillo Bravo, Esperanza Rocío; Collazos Fajardo, Alejandra; Camargo Guerrero, Mauricio
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2001
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/20937
• Acceso en línea: http://hdl.handle.net/10495/20937; https://revistas.udea.edu.co/index.php/actbio/article/view/329598
• Palabra clave: Virus de la Anemia Infecciosa Equina; Infectious Anemia Virus, Equine; Caballos; Horses; Reacción en Cadena de la Polimerasa; Polymerase Chain Reaction; Anemia infecciosa equina; Equine infectious anaemia; Genética molecular; Molecular genetics; PCR (Reacción en cadena de la polimerasa); http://aims.fao.org/aos/agrovoc/c_2630; http://aims.fao.org/aos/agrovoc/c_27577
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/2.5/co/