Procedure optimization for concentration and detection of protozoa and helminths in great volumes of water

ABSTRACT: Feces-contaminated water is a vehicle of transmission of potentially pathogenic microorganisms responsible for illnesses that represent main causes of death worldwide. A protocol for detection of intestinal parasites in great volumes of water was optimized. It includes: membrane filtration, mechanical agitation with detergent, centrifugation, chemical concentration with Mini Parasep® and microscopic examination. From samples of feces-contaminated water containing parasitic forms, a total recovery percentage of 85.7 % of parasites was achieved after tests. This procedure provides a useful alternative method that could be subjected to validation as a routine methodology in the diagnosis of microbiological water quality.

• Autores: Gutiérrez Builes, Lina Andrea; Ríos Tobón, Sandra; Agudelo Cadavid, Ruth Marina; López Jiménez, Juliana; Campo Polanco, Laura Francisca
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2018
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/47025
• Acceso en línea: https://hdl.handle.net/10495/47025; https://revmedtropical.sld.cu/index.php/medtropical/article/view/188/194
• Palabra clave: Parasitología; Parasitology; Enfermedades Transmitidas por el Agua; Waterborne Diseases; Calidad del Agua; Water Quality; https://id.nlm.nih.gov/mesh/D010274; https://id.nlm.nih.gov/mesh/D000069578; https://id.nlm.nih.gov/mesh/D060753; ODS 3: Salud y bienestar. Garantizar una vida sana y promover el bienestar de todos a todas las edades
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc/4.0/