Impacto del pigmento palúdico (Hemozoína) derivado de Plasmodium falciparum en la placenta humana

ABSTRACT: Malarial pigment or hemozoin (HZ) is a product of the hemoglobin digestion by the erythrocytic stages of Plasmodium spp. Purified malaria pigment has been implicated in several pathological processes, such as inflammation, oxidative stress, endothelial dysfunction, and immune dysregulation. Malaria infection during pregnancy can lead to abortion, premature delivery, intrauterine growth restriction and low birth weight. Although the accumulation of malaria pigment in the placental tissue is a common hallmark of placental malaria, the effect of this metabolic product on the tissue has been less studied. The present study focused on exploring the impact of natural HZ derived from Plasmodium falciparum culture on human placental explants exposed in vitro. The integrity of placental tissue was evaluated by histology using hematoxylin-eosin staining as well histochemical evaluations for collagen type I and laminin, and trophoblast membrane was evaluated by immunohistochemical staining with Cytokeratin 7. The effect of HZ in tissular viability was evaluated by measuring the apoptosis and the endocrinology function. The results indicate that natural HZ induces an increase in tissue damage, dysregulation of collagen distribution in the villous stroma, and an increase in the frequency of cellular apoptosis. Our results show a direct effect of HZ in the absence of other potential stimuli such as maternal cytokines and leukocytes or parasites, underscoring HZ alone as an important component in the pathogenesis of placental malaria. Understanding the effects of the accumulation of HZ in placental tissue will enable identification of high-risk women and may lead to discovery of new drug targets against associated adverse pregnancy outcomes.

• Autores: López Guzmán, Carolina; García Sierra, Ana María; Vásquez Cardona, Ana María
• Tipo de recurso: http://purl.org/coar/resource_type/c_5794
• Fecha de publicación: 2023
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng; spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39137
• Acceso en línea: https://hdl.handle.net/10495/39137
• Palabra clave: Plasmodium falciparum; Malaria Falciparum; Malaria, Falciparum; Enfermedades Placentarias; Placenta Diseases; Placenta; Vellosidades Coriónicas; Chorionic Villi; Hemozoína; Hemozoin; https://id.nlm.nih.gov/mesh/D010963; https://id.nlm.nih.gov/mesh/D016778; https://id.nlm.nih.gov/mesh/D010922; https://id.nlm.nih.gov/mesh/D010920; https://id.nlm.nih.gov/mesh/D002824
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-sa/2.5/co/