Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability

ABSTRACT: Iron deficiencies continue to cause significant health problems in vulnerable populations. A good strategy to combat mineral deficiency includes fortification with iron-binding peptides. This research aims to determine the optimal conditions to hydrolyze red tilapia viscera (RTV) using Alcalase 2.4 L and recovery of iron-binding protein hydrolysate. The result showed that under the optimal hydrolysis condition including pH 10, 60 ◦C, E/S ratio of 0.306 U/g protein, and substrate concentration of 8 g protein/L, the obtained hydrolysate with 42.5% degree of hydrolysis (RTVH-B), displayed the maximal iron-binding capacity of 67.1 ± 1.9%. Peptide fractionation was performed using ultrafiltration and the <1 kDa fraction (FRTVH-V) expressed the highest iron-binding capacity of 95.8 ± 1.5%. Iron content of RTVH-B and its fraction was assessed, whereas iron uptake was measured indirectly as ferritin synthesis in a Caco-2 cell model and the result showed that bioavailability of bound minerals from protein complexes was significantly higher (p < 0.05) than iron salt in its free form, increased 4.7 times for the Fe2+–RTVH-B complex. This research suggests a potential application of RTVH-B as dietary supplements to improve iron absorption.

• Autores: Zapata Montoya, Jose Edgar; Gómez Sampedro, Leidy Johanna; Gómez Grimaldos, Nathalia Andrea; López García, Gabriel; Cilla, Antonio; Alegría, Amparo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2020
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/35149
• Acceso en línea: https://hdl.handle.net/10495/35149
• Palabra clave: Células CACO-2; Caco-2 Cells; Tilapia; Proteínas Hierro-Azufre; Iron-Sulfur Proteins; Hidrólisis; Hydrolysis; Subtilisinas; Subtilisins; Disponibilidad Biológica; Biological Availability; Hierro de la Dieta; Iron, Dietary
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/