Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host
ABSTRACT: We have morphologically characterizedCandida tropicalisisolates resistant to amphotericin B (AmB). These isolates present an enlarged cell wall compared to isolates of regular susceptibility. This correlated with higher levels of β-1,3-glucan in the cell wall but not with detectable change...
- Autores:
-
Mesa Arango, Ana Cecilia
Rueda, Cristina
Román, Elvira
Quintin, Jessica
Terrón, María C.
Luque, Daniel
Netea, Mihai G.
Pla, Jesus
Zaragozaa, Oscar
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/26422
- Acceso en línea:
- http://hdl.handle.net/10495/26422
- Palabra clave:
- Anfotericina B
Amphotericin B
Antifúngicos
Antifungal Agents
Candida tropicalis
Interacciones Huésped-Patógeno
Host-Pathogen Interactions
Pared Celular
Cell Wall
Inmunidad Innata
Immunity, Innate
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by/4.0/
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|
| dc.title.spa.fl_str_mv |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| title |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| spellingShingle |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host Anfotericina B Amphotericin B Antifúngicos Antifungal Agents Candida tropicalis Interacciones Huésped-Patógeno Host-Pathogen Interactions Pared Celular Cell Wall Inmunidad Innata Immunity, Innate |
| title_short |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| title_full |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| title_fullStr |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| title_full_unstemmed |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| title_sort |
Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host |
| dc.creator.fl_str_mv |
Mesa Arango, Ana Cecilia Rueda, Cristina Román, Elvira Quintin, Jessica Terrón, María C. Luque, Daniel Netea, Mihai G. Pla, Jesus Zaragozaa, Oscar |
| dc.contributor.author.none.fl_str_mv |
Mesa Arango, Ana Cecilia Rueda, Cristina Román, Elvira Quintin, Jessica Terrón, María C. Luque, Daniel Netea, Mihai G. Pla, Jesus Zaragozaa, Oscar |
| dc.contributor.researchgroup.spa.fl_str_mv |
GRID - Grupo de Investigación Dermatológica |
| dc.subject.decs.none.fl_str_mv |
Anfotericina B Amphotericin B Antifúngicos Antifungal Agents Candida tropicalis Interacciones Huésped-Patógeno Host-Pathogen Interactions Pared Celular Cell Wall Inmunidad Innata Immunity, Innate |
| topic |
Anfotericina B Amphotericin B Antifúngicos Antifungal Agents Candida tropicalis Interacciones Huésped-Patógeno Host-Pathogen Interactions Pared Celular Cell Wall Inmunidad Innata Immunity, Innate |
| description |
ABSTRACT: We have morphologically characterizedCandida tropicalisisolates resistant to amphotericin B (AmB). These isolates present an enlarged cell wall compared to isolates of regular susceptibility. This correlated with higher levels of β-1,3-glucan in the cell wall but not with detectable changes in chitin content. In line with this, AmB-resistant strains showed reduced susceptibility to Congo red. Moreover, mitogen-activated protein kinases (MAPKs) involved in cell integrity were already activated during regular growth in these strains. Finally, we investigated the response elicited by human blood cells and found that AmB-resistant strains induced a stronger proinflammatory response than susceptible strains. In agreement, AmB-resistant strains also induced stronger melanization ofGalleria mellonellalarvae, indicating that the effect of alterations of the cell wall on the immune response is conserved in different types of hosts. Our results suggest that resistance to AmB is associated with pleiotropic mechanisms that might have important consequences, not only for the efficacy of the treatment but also for the immune response elicited by the host. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2022-03-06T19:53:49Z |
| dc.date.available.none.fl_str_mv |
2022-03-06T19:53:49Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Mesa-Arango AC, Rueda C, Román E, Quintin J, Terrón MC, Luque D, Netea MG, Pla J, Zaragoza O. Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host. Antimicrob Agents Chemother. 2016 Mar 25;60(4):2326-35. doi: 10.1128/AAC.02681-15. |
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0066-4804 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/26422 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/AAC.02681-15. |
| dc.identifier.eissn.none.fl_str_mv |
1098-6596 |
| identifier_str_mv |
Mesa-Arango AC, Rueda C, Román E, Quintin J, Terrón MC, Luque D, Netea MG, Pla J, Zaragoza O. Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host. Antimicrob Agents Chemother. 2016 Mar 25;60(4):2326-35. doi: 10.1128/AAC.02681-15. 0066-4804 10.1128/AAC.02681-15. 1098-6596 |
| url |
http://hdl.handle.net/10495/26422 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Antimicrob. Agents. Chemother. |
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2335 |
| dc.relation.citationissue.spa.fl_str_mv |
4 |
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2326 |
| dc.relation.citationvolume.spa.fl_str_mv |
60 |
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Antimicrobial Agents and Chemotherapy |
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https://creativecommons.org/licenses/by/4.0/ |
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http://creativecommons.org/licenses/by/2.5/co/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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10 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
American Society for Microbiology |
| dc.publisher.place.spa.fl_str_mv |
Washington, Estados Unidos |
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Universidad de Antioquia |
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Mesa Arango, Ana CeciliaRueda, CristinaRomán, ElviraQuintin, JessicaTerrón, María C.Luque, DanielNetea, Mihai G.Pla, JesusZaragozaa, OscarGRID - Grupo de Investigación Dermatológica2022-03-06T19:53:49Z2022-03-06T19:53:49Z2016Mesa-Arango AC, Rueda C, Román E, Quintin J, Terrón MC, Luque D, Netea MG, Pla J, Zaragoza O. Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host. Antimicrob Agents Chemother. 2016 Mar 25;60(4):2326-35. doi: 10.1128/AAC.02681-15.0066-4804http://hdl.handle.net/10495/2642210.1128/AAC.02681-15.1098-6596ABSTRACT: We have morphologically characterizedCandida tropicalisisolates resistant to amphotericin B (AmB). These isolates present an enlarged cell wall compared to isolates of regular susceptibility. This correlated with higher levels of β-1,3-glucan in the cell wall but not with detectable changes in chitin content. In line with this, AmB-resistant strains showed reduced susceptibility to Congo red. Moreover, mitogen-activated protein kinases (MAPKs) involved in cell integrity were already activated during regular growth in these strains. Finally, we investigated the response elicited by human blood cells and found that AmB-resistant strains induced a stronger proinflammatory response than susceptible strains. In agreement, AmB-resistant strains also induced stronger melanization ofGalleria mellonellalarvae, indicating that the effect of alterations of the cell wall on the immune response is conserved in different types of hosts. Our results suggest that resistance to AmB is associated with pleiotropic mechanisms that might have important consequences, not only for the efficacy of the treatment but also for the immune response elicited by the host.COL005083910application/pdfengAmerican Society for MicrobiologyWashington, Estados Unidoshttps://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the HostArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAnfotericina BAmphotericin BAntifúngicosAntifungal AgentsCandida tropicalisInteracciones Huésped-PatógenoHost-Pathogen InteractionsPared CelularCell WallInmunidad InnataImmunity, InnateAntimicrob. Agents. 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