Cell Wall Changes in Amphotericin B-Resistant Strains from Candida tropicalis and Relationship with the Immune Responses Elicited by the Host

ABSTRACT: We have morphologically characterizedCandida tropicalisisolates resistant to amphotericin B (AmB). These isolates present an enlarged cell wall compared to isolates of regular susceptibility. This correlated with higher levels of β-1,3-glucan in the cell wall but not with detectable changes in chitin content. In line with this, AmB-resistant strains showed reduced susceptibility to Congo red. Moreover, mitogen-activated protein kinases (MAPKs) involved in cell integrity were already activated during regular growth in these strains. Finally, we investigated the response elicited by human blood cells and found that AmB-resistant strains induced a stronger proinflammatory response than susceptible strains. In agreement, AmB-resistant strains also induced stronger melanization ofGalleria mellonellalarvae, indicating that the effect of alterations of the cell wall on the immune response is conserved in different types of hosts. Our results suggest that resistance to AmB is associated with pleiotropic mechanisms that might have important consequences, not only for the efficacy of the treatment but also for the immune response elicited by the host.

• Autores: Mesa Arango, Ana Cecilia; Rueda, Cristina; Román, Elvira; Quintin, Jessica; Terrón, María C.; Luque, Daniel; Netea, Mihai G.; Pla, Jesus; Zaragozaa, Oscar
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2016
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/26422
• Acceso en línea: http://hdl.handle.net/10495/26422
• Palabra clave: Anfotericina B; Amphotericin B; Antifúngicos; Antifungal Agents; Candida tropicalis; Interacciones Huésped-Patógeno; Host-Pathogen Interactions; Pared Celular; Cell Wall; Inmunidad Innata; Immunity, Innate
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/