Selective interaction of lansoprazole and astemizole with tau polymers: potential new clinical use in diagnosis of Alzheimer's disease

ABSTRACT: We describe the interactions of two benzimidazole derivatives, astemizole (AST) and lansoprazole (LNS), with anomalous aggregates of tau protein (neurofibrillary tangles). Interestingly, these compounds, with important medical applications in the treatment of allergies and gastrointestinal disorders respectively, specifically bind to aggregated variants of tau protein and to paired helical filaments isolated from brains of Alzheimer's disease (AD) patients. These ligands appear to be a powerful tool to tag brain-isolated tau-aggregates and heparin-induced polymers of recombinant tau. The interactions of AST and LNS with tau aggregates were assessed by classical radioligand assays, surface plasmon resonance, and bioinformatic approaches. The affinity of AST and LNS for tau aggregates was comparatively higher than that for amyloid-β polymers according to our data. This is relevant since senile plaques are also abundant but are not pathognomonic in AD patients. Immunochemical studies on paired helical filaments from brains of AD patients and surface plasmon resonance studies confirm these findings. The capacity of these drugs to penetrate the blood-brain barrier was evaluated: i) in vitro by parallel artificial membrane permeability assay followed by experimental Log P determinations; and ii) in vivo by pharmacokinetic studies comparing distribution profiles in blood and brain of mice using HPLC/UV. Importantly, our studies indicate that the brain/blood concentration ratios for these compounds were suitable for their use as PET radiotracers. Since neurofibrillary tangles are positively correlated with cognitive impairment, we concluded that LNS and AST have a great potential in PET neuroimaing for in vivo early detection of AD and in reducing the formation of neurofibrillary tangles.

• Autores: Alzate Morales, Jans Humberto; Rojo, Leonel E.; Saavedra, Iván N.; Davies, Peter; Maccioni, Ricardo B.
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2010
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/35961
• Acceso en línea: https://hdl.handle.net/10495/35961
• Palabra clave: Enfermedad de Alzheimer; Alzheimer Disease; Astemizol - química; Astemizole - chemistry; Cromatografía Líquida de Alta Presión; Chromatography, High Pressure Liquid; Biología Computacional; Computational Biology; Relación Dosis-Respuesta a Droga; Dose-Response Relationship, Drug; Técnicas Electroquímicas; Electrochemical Techniques; Tomografía con Microscopio Electrónico - métodos; Electron Microscope Tomography - methods; Inhibidores Enzimáticos - química; Enzyme Inhibitors - chemistry; Lansoprazol; Lansoprazole; Modelos Moleculares; Models, Molecular; Ovillos Neurofibrilares; Neurofibrillary Tangles; Resonancia por Plasmón de Superficie; Surface Plasmon Resonance
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc/4.0/