Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins

ABSTRACT: Aedes aegypti is the primary mosquito vector of several human arboviruses, including the dengue virus (DENV). Vector control is the principal intervention to decrease the transmission of these viruses. The characterization of molecules involved in the mosquito physiological responses to blood-feeding may help identify novel targets useful in designing effective control strategies. In this study, we evaluated the in vivo effect of feeding adult female mosquitoes with human red blood cells reconstituted with either heat-inactivated (IB) or normal plasma (NB). The RNA-seq based transcript expression of IB and NB mosquitoes was compared against sugar-fed (SF) mosquitoes. In in vitro experiments, we treated Aag2 cells with a recombinant version of complement proteins (hC3 or hC5a) and compared transcript expression to untreated control cells after 24 h. The transcript expression analysis revealed that human complement proteins modulate approximately 2300 transcripts involved in multiple biological functions, including immunity. We also found 161 upregulated and 168 downregulated transcripts differentially expressed when human complement protein C3 (hC3) and human complement protein C5a (hC5a) treated cells were compared to the control untreated cells. We conclude that active human complement induces significant changes to the transcriptome of Ae. aegypti mosquitoes, which may influence the physiology of these arthropods.

• Autores: Calle Tobón, Arley Fernando; Rúa Uribe, Guillermo León; Giraldo Calderón, Gloria I.; Rozo López, Paula; Colpitts, Tonya M.; Park, Yoonseong; Londoño Rentería, Berlin
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2021
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/45155
• Acceso en línea: https://hdl.handle.net/10495/45155
• Palabra clave: Aedes; Complemento C3; Complement C3; Complemento C5a; Complement C5a; Mosquitos Vectores; Mosquito Vectors; Transcriptoma; Transcriptome; https://id.nlm.nih.gov/mesh/D000330; https://id.nlm.nih.gov/mesh/D003176; https://id.nlm.nih.gov/mesh/D015936; https://id.nlm.nih.gov/mesh/D000072138; https://id.nlm.nih.gov/mesh/D059467
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/