Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection
ABSTRACT: Epidemics of dengue, an acute and potentially severe disease caused by mosquito-borne dengue virus (DENV), pose a major challenge to clinicians and health care services across the sub(tropics). Severe disease onset is associated with a dysregulated inflammatory response to the virus, and t...
- Autores:
-
Castillo Ramírez, Jorge Andrés
Giraldo Giraldo, Diana Marcela
Rodenhuis Zybert, Izabela Agata
Smit, Jolanda
Urcuqui Inchima, Silvio
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2022
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/29734
- Acceso en línea:
- https://hdl.handle.net/10495/29734
- Palabra clave:
- Virus del Dengue
Dengue Virus
Macrófagos
Macrophages
Inmunidad Innata
Immunity, Innate
Vitamina D
Vitamin D
LL-37
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| title |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| spellingShingle |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection Virus del Dengue Dengue Virus Macrófagos Macrophages Inmunidad Innata Immunity, Innate Vitamina D Vitamin D LL-37 |
| title_short |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| title_full |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| title_fullStr |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| title_full_unstemmed |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| title_sort |
Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection |
| dc.creator.fl_str_mv |
Castillo Ramírez, Jorge Andrés Giraldo Giraldo, Diana Marcela Rodenhuis Zybert, Izabela Agata Smit, Jolanda Urcuqui Inchima, Silvio |
| dc.contributor.author.none.fl_str_mv |
Castillo Ramírez, Jorge Andrés Giraldo Giraldo, Diana Marcela Rodenhuis Zybert, Izabela Agata Smit, Jolanda Urcuqui Inchima, Silvio |
| dc.contributor.researchgroup.spa.fl_str_mv |
Inmunovirología |
| dc.subject.decs.none.fl_str_mv |
Virus del Dengue Dengue Virus Macrófagos Macrophages Inmunidad Innata Immunity, Innate Vitamina D Vitamin D |
| topic |
Virus del Dengue Dengue Virus Macrófagos Macrophages Inmunidad Innata Immunity, Innate Vitamina D Vitamin D LL-37 |
| dc.subject.proposal.spa.fl_str_mv |
LL-37 |
| description |
ABSTRACT: Epidemics of dengue, an acute and potentially severe disease caused by mosquito-borne dengue virus (DENV), pose a major challenge to clinicians and health care services across the sub(tropics). Severe disease onset is associated with a dysregulated inflammatory response to the virus, and there are currently no drugs to alleviate disease symptoms. LL-37 is a potent antimicrobial peptide with a wide range of immunoregulatory properties. In this study, we assessed the effect of LL-37 on DENV-2-induced responses in human monocyte-derived macrophages (MDMs). We show that simultaneous exposure of exogenous LL-37 and DENV-2 resulted in reduced replication of the virus in MDMs, while the addition of LL-37 postexposure to DENV-2 did not. Interestingly, the latter condition reduced the production of IL-6 and increased the expression of genes involved in virus sensing and antiviral response. Finally, we demonstrate that low endogenous levels and limited production of LL-37 in MDMs in response to DENV-2 infection can be increased by differentiating MDMs in the presence of Vitamin D (VitD3). Taken together, this study demonstrates that in addition to its antimicrobial properties, LL-37 has immunomodulatory properties in the curse of DENV infection and its production can be increased by VitD3. |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-07-14T15:44:10Z |
| dc.date.available.none.fl_str_mv |
2022-07-14T15:44:10Z |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/acceptedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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acceptedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Castillo JA, Giraldo DM, Smit JM, Rodenhuis-Zybert IA, Urcuqui-Inchima S. Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection. Pathog Dis. 2022 May 23;80(1):ftac014. doi: 10.1093/femspd/ftac014. |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/29734 |
| dc.identifier.doi.none.fl_str_mv |
10.1093/femspd/ftac014 |
| dc.identifier.eissn.none.fl_str_mv |
2049-632X |
| identifier_str_mv |
Castillo JA, Giraldo DM, Smit JM, Rodenhuis-Zybert IA, Urcuqui-Inchima S. Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection. Pathog Dis. 2022 May 23;80(1):ftac014. doi: 10.1093/femspd/ftac014. 10.1093/femspd/ftac014 2049-632X |
| url |
https://hdl.handle.net/10495/29734 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Pathog. Dis. |
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11 |
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1 |
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1 |
| dc.relation.citationvolume.spa.fl_str_mv |
80 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Pathogens and Disease |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia |
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openAccess |
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application/pdf |
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Oxford University Press |
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Oxford, Estados Unidos |
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Castillo Ramírez, Jorge AndrésGiraldo Giraldo, Diana MarcelaRodenhuis Zybert, Izabela AgataSmit, JolandaUrcuqui Inchima, SilvioInmunovirología2022-07-14T15:44:10Z2022-07-14T15:44:10Z2022Castillo JA, Giraldo DM, Smit JM, Rodenhuis-Zybert IA, Urcuqui-Inchima S. Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection. Pathog Dis. 2022 May 23;80(1):ftac014. doi: 10.1093/femspd/ftac014.https://hdl.handle.net/10495/2973410.1093/femspd/ftac0142049-632XABSTRACT: Epidemics of dengue, an acute and potentially severe disease caused by mosquito-borne dengue virus (DENV), pose a major challenge to clinicians and health care services across the sub(tropics). Severe disease onset is associated with a dysregulated inflammatory response to the virus, and there are currently no drugs to alleviate disease symptoms. LL-37 is a potent antimicrobial peptide with a wide range of immunoregulatory properties. In this study, we assessed the effect of LL-37 on DENV-2-induced responses in human monocyte-derived macrophages (MDMs). We show that simultaneous exposure of exogenous LL-37 and DENV-2 resulted in reduced replication of the virus in MDMs, while the addition of LL-37 postexposure to DENV-2 did not. Interestingly, the latter condition reduced the production of IL-6 and increased the expression of genes involved in virus sensing and antiviral response. Finally, we demonstrate that low endogenous levels and limited production of LL-37 in MDMs in response to DENV-2 infection can be increased by differentiating MDMs in the presence of Vitamin D (VitD3). Taken together, this study demonstrates that in addition to its antimicrobial properties, LL-37 has immunomodulatory properties in the curse of DENV infection and its production can be increased by VitD3.COL001244411application/pdfengOxford University PressOxford, Estados Unidoshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Colombiahttp://purl.org/coar/access_right/c_abf2Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infectionArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionVirus del DengueDengue VirusMacrófagosMacrophagesInmunidad InnataImmunity, InnateVitamina DVitamin DLL-37Pathog. 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