In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6
ABSTRACT: Colorectal cancer mortality rate and highly altered proteins from the Wnt/β-catenin pathway increase the scientific community’s interest in finding alternatives for prevention and treatment. This study aims to determine the biological effect of chlorogenic acid (CGA) on two colorectal canc...
- Autores:
-
Henao Castañeda, Isabel Cristina
Vélez Vargas, Laura Catalina
Santa González, Gloria Angélica
Pedroza Díaz, Nini Johanna
Uribe, Diego
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/40141
- Acceso en línea:
- https://hdl.handle.net/10495/40141
- Palabra clave:
- Ácido Clorogénico
Chlorogenic Acid
Neoplasias Colorrectales
Colorectal Neoplasms
Apoptosis
beta Catenina
beta Catenin
Proteína-6 Relacionada a Receptor de Lipoproteína de Baja Densidad
Low Density Lipoprotein Receptor-Related Protein-6
https://id.nlm.nih.gov/mesh/D002726
https://id.nlm.nih.gov/mesh/D015179
https://id.nlm.nih.gov/mesh/D017209
https://id.nlm.nih.gov/mesh/D051176
https://id.nlm.nih.gov/mesh/D060488
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| title |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| spellingShingle |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 Ácido Clorogénico Chlorogenic Acid Neoplasias Colorrectales Colorectal Neoplasms Apoptosis beta Catenina beta Catenin Proteína-6 Relacionada a Receptor de Lipoproteína de Baja Densidad Low Density Lipoprotein Receptor-Related Protein-6 https://id.nlm.nih.gov/mesh/D002726 https://id.nlm.nih.gov/mesh/D015179 https://id.nlm.nih.gov/mesh/D017209 https://id.nlm.nih.gov/mesh/D051176 https://id.nlm.nih.gov/mesh/D060488 |
| title_short |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| title_full |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| title_fullStr |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| title_full_unstemmed |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| title_sort |
In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6 |
| dc.creator.fl_str_mv |
Henao Castañeda, Isabel Cristina Vélez Vargas, Laura Catalina Santa González, Gloria Angélica Pedroza Díaz, Nini Johanna Uribe, Diego |
| dc.contributor.author.none.fl_str_mv |
Henao Castañeda, Isabel Cristina Vélez Vargas, Laura Catalina Santa González, Gloria Angélica Pedroza Díaz, Nini Johanna Uribe, Diego |
| dc.contributor.researchgroup.spa.fl_str_mv |
Productos Naturales Marinos |
| dc.subject.decs.none.fl_str_mv |
Ácido Clorogénico Chlorogenic Acid Neoplasias Colorrectales Colorectal Neoplasms Apoptosis beta Catenina beta Catenin Proteína-6 Relacionada a Receptor de Lipoproteína de Baja Densidad Low Density Lipoprotein Receptor-Related Protein-6 |
| topic |
Ácido Clorogénico Chlorogenic Acid Neoplasias Colorrectales Colorectal Neoplasms Apoptosis beta Catenina beta Catenin Proteína-6 Relacionada a Receptor de Lipoproteína de Baja Densidad Low Density Lipoprotein Receptor-Related Protein-6 https://id.nlm.nih.gov/mesh/D002726 https://id.nlm.nih.gov/mesh/D015179 https://id.nlm.nih.gov/mesh/D017209 https://id.nlm.nih.gov/mesh/D051176 https://id.nlm.nih.gov/mesh/D060488 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D002726 https://id.nlm.nih.gov/mesh/D015179 https://id.nlm.nih.gov/mesh/D017209 https://id.nlm.nih.gov/mesh/D051176 https://id.nlm.nih.gov/mesh/D060488 |
| description |
ABSTRACT: Colorectal cancer mortality rate and highly altered proteins from the Wnt/β-catenin pathway increase the scientific community’s interest in finding alternatives for prevention and treatment. This study aims to determine the biological effect of chlorogenic acid (CGA) on two colorectal cancer cell lines, HT-29 and SW480, and its interactions with β-catenin and LRP6 to elucidate a possible modulatory mechanism on the Wnt/β-catenin pathway. These effects were determined by propidium iodide and DiOC6 for mitochondrial membrane permeability, MitoTracker cycle phases, and molecular docking for protein–ligand interactions and binding affinity. Here, it was found that CGA at 2000 μM significantly affects cell viability and causes DNA fragmentation in SW480 cells rather than in HT-29 cells, but in both cell lines, it induces ROS production. Additionally, CGA has similar affinity and interactions for LRP6 as niclosamide but has a higher affinity for both β-catenin sites than C2 and iCRT14. These results suggest a possible modulatory role of CGA over the Wnt/β-catenin pathway in colorectal cancer. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-06-19T13:33:50Z |
| dc.date.available.none.fl_str_mv |
2024-06-19T13:33:50Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Vélez-Vargas, L.C.; Santa-González, G.A.; Uribe, D.; Henao-Castañeda, I.C.; Pedroza-Díaz, J. In Vitro and In Silico Study on the Impact of Chlorogenic Acid in Colorectal Cancer Cells: Proliferation, Apoptosis, and Interaction with β-Catenin and LRP6. Pharmaceuticals 2023, 16, 276. https://doi.org/10.3390/ph16020276 |
| dc.identifier.issn.none.fl_str_mv |
1424-8247 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/40141 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/ph16020276 |
| identifier_str_mv |
Vélez-Vargas, L.C.; Santa-González, G.A.; Uribe, D.; Henao-Castañeda, I.C.; Pedroza-Díaz, J. In Vitro and In Silico Study on the Impact of Chlorogenic Acid in Colorectal Cancer Cells: Proliferation, Apoptosis, and Interaction with β-Catenin and LRP6. Pharmaceuticals 2023, 16, 276. https://doi.org/10.3390/ph16020276 1424-8247 10.3390/ph16020276 |
| url |
https://hdl.handle.net/10495/40141 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Pharmaceuticals |
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19 |
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2 |
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1 |
| dc.relation.citationvolume.spa.fl_str_mv |
16 |
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Pharmaceuticals |
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http://creativecommons.org/licenses/by/2.5/co/ |
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https://creativecommons.org/licenses/by/4.0/ |
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19 páginas |
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Henao Castañeda, Isabel CristinaVélez Vargas, Laura CatalinaSanta González, Gloria AngélicaPedroza Díaz, Nini JohannaUribe, DiegoProductos Naturales Marinos2024-06-19T13:33:50Z2024-06-19T13:33:50Z2023Vélez-Vargas, L.C.; Santa-González, G.A.; Uribe, D.; Henao-Castañeda, I.C.; Pedroza-Díaz, J. In Vitro and In Silico Study on the Impact of Chlorogenic Acid in Colorectal Cancer Cells: Proliferation, Apoptosis, and Interaction with β-Catenin and LRP6. Pharmaceuticals 2023, 16, 276. https://doi.org/10.3390/ph160202761424-8247https://hdl.handle.net/10495/4014110.3390/ph16020276ABSTRACT: Colorectal cancer mortality rate and highly altered proteins from the Wnt/β-catenin pathway increase the scientific community’s interest in finding alternatives for prevention and treatment. This study aims to determine the biological effect of chlorogenic acid (CGA) on two colorectal cancer cell lines, HT-29 and SW480, and its interactions with β-catenin and LRP6 to elucidate a possible modulatory mechanism on the Wnt/β-catenin pathway. These effects were determined by propidium iodide and DiOC6 for mitochondrial membrane permeability, MitoTracker cycle phases, and molecular docking for protein–ligand interactions and binding affinity. Here, it was found that CGA at 2000 μM significantly affects cell viability and causes DNA fragmentation in SW480 cells rather than in HT-29 cells, but in both cell lines, it induces ROS production. Additionally, CGA has similar affinity and interactions for LRP6 as niclosamide but has a higher affinity for both β-catenin sites than C2 and iCRT14. These results suggest a possible modulatory role of CGA over the Wnt/β-catenin pathway in colorectal cancer.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MincienciasInstituto Tecnológico MetropolitanoCOL001504319 páginasapplication/pdf - application/epubengMDPIBasilea, Suizahttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2In vitro and in silico study on the impact of chlorogenic acid in colorectal cancer cells: proliferation, apoptosis, and interaction with β-Catenin and LRP6Artículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionÁcido ClorogénicoChlorogenic AcidNeoplasias ColorrectalesColorectal NeoplasmsApoptosisbeta Cateninabeta CateninProteína-6 Relacionada a Receptor de Lipoproteína de Baja DensidadLow Density Lipoprotein Receptor-Related Protein-6https://id.nlm.nih.gov/mesh/D002726https://id.nlm.nih.gov/mesh/D015179https://id.nlm.nih.gov/mesh/D017209https://id.nlm.nih.gov/mesh/D051176https://id.nlm.nih.gov/mesh/D060488Pharmaceuticals192116Pharmaceuticals115080763215 CT 811-2018RoR:03fd5ne08RoR:03zb5p722PublicationORIGINALHenaoIsabel_2023_In_Vitro_Study_Impact_Chlorogenic_Acid.pdfHenaoIsabel_2023_In_Vitro_Study_Impact_Chlorogenic_Acid.pdfArtículo de investigaciónapplication/pdf5720331https://bibliotecadigital.udea.edu.co/bitstreams/c52927e2-0d20-4b1a-a440-794ec547d836/download0f5a4da1985e58b2634b459be9754847MD51trueAnonymousREADHenaoIsabel_2023_In_Vitro_Study_Impact_Chlorogenic_Acid.epubHenaoIsabel_2023_In_Vitro_Study_Impact_Chlorogenic_Acid.epubArtículo de investigaciónapplication/epub+zip15325589https://bibliotecadigital.udea.edu.co/bitstreams/dc8afa2c-e984-4da3-a5f8-728d8f5c46c1/download9d2781215afebe6c2cb64bbe970f0d54MD52falseAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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