El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6
ABSTRACT: Dehydroepiandrosterone sulfate (DHEA-S) is a neurosteroid that has effects such as neuromodulator of synaptic transmission and neuroprotection. The specific signaling pathways for these effects are not elucidated yet. Given that, some neurosteroids act through the activation of ionotropic...
- Autores:
-
Ospina Villegas, Carolina
Sepúlveda Falla, Diego Alonso
Barrera Ocampo, Alvaro Andrés
Cardona Gómez, Gloria Patricia
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2010
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/12653
- Acceso en línea:
- http://hdl.handle.net/10495/12653
- Palabra clave:
- AMPA
Cerebelo
Corteza
DHEA
DHEAS
GluR2/3
GluR2
Hipocampo
Hipotálamo
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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| dc.title.spa.fl_str_mv |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| dc.title.translated.spa.fl_str_mv |
DHEAS increases levels of GluR2/3 and GluR2, AMPA receptor subunits, in C57BL/6 mice hippocampus |
| title |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| spellingShingle |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 AMPA Cerebelo Corteza DHEA DHEAS GluR2/3 GluR2 Hipocampo Hipotálamo |
| title_short |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| title_full |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| title_fullStr |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| title_full_unstemmed |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| title_sort |
El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6 |
| dc.creator.fl_str_mv |
Ospina Villegas, Carolina Sepúlveda Falla, Diego Alonso Barrera Ocampo, Alvaro Andrés Cardona Gómez, Gloria Patricia |
| dc.contributor.author.none.fl_str_mv |
Ospina Villegas, Carolina Sepúlveda Falla, Diego Alonso Barrera Ocampo, Alvaro Andrés Cardona Gómez, Gloria Patricia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Neurociencias de Antioquia |
| dc.subject.none.fl_str_mv |
AMPA Cerebelo Corteza DHEA DHEAS GluR2/3 GluR2 Hipocampo Hipotálamo |
| topic |
AMPA Cerebelo Corteza DHEA DHEAS GluR2/3 GluR2 Hipocampo Hipotálamo |
| description |
ABSTRACT: Dehydroepiandrosterone sulfate (DHEA-S) is a neurosteroid that has effects such as neuromodulator of synaptic transmission and neuroprotection. The specific signaling pathways for these effects are not elucidated yet. Given that, some neurosteroids act through the activation of ionotropic glutamate receptors, therefore the effect of DHEA-S on the subunits GluR2 and GluR3 of the AMPA receptor was evaluated. Either DHEA-S or a control substance was administered to C57/BL6 mice. Subunit expression of the AMPA receptor was analyzed by Western blotting. Results show that long-term DHEA-S administration to C57/BL6 mice, increases the protein levels of the subunits GluR2 and GluR2/3 of the AMPA receptors located in the hippocampus. Due to the role of AMPA receptor, specifically GluR2 subunit in the regulation of intracellular calcium levels, cellular apoptosis, and synaptic plasticity, the study of neurosteroids as a therapeutic strategy in neurodegenerative diseases and cerebrovascular events is very relevant. |
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2010 |
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2010 |
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2019-12-19T21:14:50Z |
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2019-12-19T21:14:50Z |
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Artículo de investigación |
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Ospina Villegas C, Sepúlveda-Falla D, Barrera-Ocampo AA, Cardona-Gómez GP. El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6. Iatreia. 2010 Jun;23(1): 99-106. |
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0121-0793 |
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http://hdl.handle.net/10495/12653 |
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2011-7965 |
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Ospina Villegas C, Sepúlveda-Falla D, Barrera-Ocampo AA, Cardona-Gómez GP. El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6. Iatreia. 2010 Jun;23(1): 99-106. 0121-0793 2011-7965 |
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http://hdl.handle.net/10495/12653 |
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spa |
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Iatreia |
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23 |
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Universidad de Antioquia, Facultad de Medicina |
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Ospina Villegas, CarolinaSepúlveda Falla, Diego AlonsoBarrera Ocampo, Alvaro AndrésCardona Gómez, Gloria PatriciaGrupo de Neurociencias de Antioquia2019-12-19T21:14:50Z2019-12-19T21:14:50Z2010Ospina Villegas C, Sepúlveda-Falla D, Barrera-Ocampo AA, Cardona-Gómez GP. El DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6. Iatreia. 2010 Jun;23(1): 99-106.0121-0793http://hdl.handle.net/10495/126532011-7965ABSTRACT: Dehydroepiandrosterone sulfate (DHEA-S) is a neurosteroid that has effects such as neuromodulator of synaptic transmission and neuroprotection. The specific signaling pathways for these effects are not elucidated yet. Given that, some neurosteroids act through the activation of ionotropic glutamate receptors, therefore the effect of DHEA-S on the subunits GluR2 and GluR3 of the AMPA receptor was evaluated. Either DHEA-S or a control substance was administered to C57/BL6 mice. Subunit expression of the AMPA receptor was analyzed by Western blotting. Results show that long-term DHEA-S administration to C57/BL6 mice, increases the protein levels of the subunits GluR2 and GluR2/3 of the AMPA receptors located in the hippocampus. Due to the role of AMPA receptor, specifically GluR2 subunit in the regulation of intracellular calcium levels, cellular apoptosis, and synaptic plasticity, the study of neurosteroids as a therapeutic strategy in neurodegenerative diseases and cerebrovascular events is very relevant.RESUMEN: El DHEAS es un neuroesteroide con efecto neuromodulador de la transmisión sináptica y en la neuroprotección, sin embargo las vías moleculares a través de las cuales se inducen estos cambios no están completamente claras. Como varios de los neuroesteroides actúan a través de los recetores ionotrópicos de glutamato, se evaluó el efecto del DHEAS en las subunidades GluR2 y GluR3 del receptor AMPA para esclarecer sus efectos. Con este fin se administró DHEAS o una sustancia control durante 7 días a ratones C57/BL6. La expresión de las subunidades se evaluó por Western blotting. Los resultados presentados muestran que la administración prolongada de 40mg/kg/día de DHEAS a ratones C57/BL6 produce un incremento en los niveles de proteína de las subunidades GluR2/3 y GluR2 del receptor AMPA en el hipocampo. Dado el papel específico que juega la subunidad GluR2 del receptor AMPA en el control de la entrada de calcio durante los procesos de muerte celular y de plasticidad sináptica, este hallazgo contribuye al estudio de los neuroesteroides como una estrategia terapéutica relevante en enfermedades neurodegenerativas y eventos cerebrovasculares.7application/pdfspaUniversidad de Antioquia, Facultad de MedicinaMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/4.0/https://creativecommons.org/licenses/by-nc-sa/2.5/co/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2AMPACerebeloCortezaDHEADHEASGluR2/3GluR2HipocampoHipotálamoEl DHEAS incrementa la expresión de GluR2/3 y GLUR2 del receptor AMPA en el hipocampo de ratones C57/BL6DHEAS increases levels of GluR2/3 and GluR2, AMPA receptor subunits, in C57BL/6 mice hippocampusArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionIatreia106223IatreiaPublicationORIGINALOspinaCarolina_2010_DHEASIncrementaHipocampo.pdfOspinaCarolina_2010_DHEASIncrementaHipocampo.pdfArtículo de investigaciónapplication/pdf649837https://bibliotecadigital.udea.edu.co/bitstreams/08ab7ed8-9085-4add-9c4d-496ba7bd4b37/downloade03c90c3f0558f84ffe457a85d1df814MD51trueAnonymousREADCC-LICENSElicense_urllicense_urltext/plain; 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