HSP90 is a target protein for ubiquitination in Giardia intestinalis
ABSTRACT: Previous studies have demonstrated the existence and expression of genes essential to the process of protein ubiquitination in Giardia intestinalis, indicating that the ubiquitin-proteasome system may be involved in the degradation of proteins during its life cycle of the parasite. In this...
- Autores:
-
Chaparro Gutiérrez, Jenny Jovana
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2011
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/8001
- Acceso en línea:
- http://hdl.handle.net/10495/8001
- Palabra clave:
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/2.5/co/
| id |
UDEA2_6ff9fd2dbb949ac6e5c2e0272f5c244b |
|---|---|
| oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/8001 |
| network_acronym_str |
UDEA2 |
| network_name_str |
Repositorio UdeA |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| dc.title.translated.spa.fl_str_mv |
HSP90 es una proteína blanco de ubiquitinación en Giardia intestinalis |
| title |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| spellingShingle |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| title_short |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| title_full |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| title_fullStr |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| title_full_unstemmed |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| title_sort |
HSP90 is a target protein for ubiquitination in Giardia intestinalis |
| dc.creator.fl_str_mv |
Chaparro Gutiérrez, Jenny Jovana |
| dc.contributor.author.none.fl_str_mv |
Chaparro Gutiérrez, Jenny Jovana |
| description |
ABSTRACT: Previous studies have demonstrated the existence and expression of genes essential to the process of protein ubiquitination in Giardia intestinalis, indicating that the ubiquitin-proteasome system may be involved in the degradation of proteins during its life cycle of the parasite. In this study, purification of ubiquitin was conducted from protein extracts of G. intestinalis trophozoites. Then, an anti-ubiquitin specific antibody was obtained to standardize an assay for the detection and evaluation of ubiquitination patterns. Finally, HSP90 was identified as an ubiquitinated protein in this protozoan. This post-translational modification could have regulatory effects associated with the functionality of the protein or its turnover to regulate key molecular events during the parasite’s life cycle. |
| publishDate |
2011 |
| dc.date.issued.none.fl_str_mv |
2011 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T16:57:24Z |
| dc.date.available.none.fl_str_mv |
2017-08-23T16:57:24Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Chaparro-Gutiérrez JJ, Wasserman M. HSP90 is a target protein for ubiquitination in Giardia intestinalis. Rev. colomb. cienc. pecu. 2011 Jun; 24(2): 107-115 |
| dc.identifier.issn.none.fl_str_mv |
0120-0690 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/8001 |
| dc.identifier.eissn.none.fl_str_mv |
2256-2958 |
| identifier_str_mv |
Chaparro-Gutiérrez JJ, Wasserman M. HSP90 is a target protein for ubiquitination in Giardia intestinalis. Rev. colomb. cienc. pecu. 2011 Jun; 24(2): 107-115 0120-0690 2256-2958 |
| url |
http://hdl.handle.net/10495/8001 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
| dc.relation.citationendpage.spa.fl_str_mv |
115 |
| dc.relation.citationissue.spa.fl_str_mv |
2 |
| dc.relation.citationstartpage.spa.fl_str_mv |
107 |
| dc.relation.citationvolume.spa.fl_str_mv |
24 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Colombiana de Ciencias Pecuarias |
| dc.rights.uri.*.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.rights.accessrights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/co/ https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Ciencias Agrarias |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstreams/8afc0b6e-5fef-4062-bc6d-fa66356f97d3/download https://bibliotecadigital.udea.edu.co/bitstreams/f2850924-9017-418d-ac6c-47a8898aef52/download https://bibliotecadigital.udea.edu.co/bitstreams/03c1c8dd-9a82-471e-ae14-145d2f24711e/download https://bibliotecadigital.udea.edu.co/bitstreams/e190a4fd-2643-4f33-8fda-98b75195c08f/download https://bibliotecadigital.udea.edu.co/bitstreams/f3d4d8a8-d3b3-4772-9471-a56a41b3f4c2/download https://bibliotecadigital.udea.edu.co/bitstreams/d99241e4-fdb3-4e4f-b5ea-ccedc8e8af22/download https://bibliotecadigital.udea.edu.co/bitstreams/69b3c360-cd7d-400e-884b-362c346fd08d/download |
| bitstream.checksum.fl_str_mv |
4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 8a4605be74aa9ea9d79846c1fba20a33 f711d25e58172efded88a95912371d54 1250ee797e35285ff8e79b0ee46b7750 04bdc382f398fce217cc884fc3b721d0 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional de la Universidad de Antioquia |
| repository.mail.fl_str_mv |
aplicacionbibliotecadigitalbiblioteca@udea.edu.co |
| _version_ |
1851052593232478208 |
| spelling |
Chaparro Gutiérrez, Jenny Jovana2017-08-23T16:57:24Z2017-08-23T16:57:24Z2011Chaparro-Gutiérrez JJ, Wasserman M. HSP90 is a target protein for ubiquitination in Giardia intestinalis. Rev. colomb. cienc. pecu. 2011 Jun; 24(2): 107-1150120-0690http://hdl.handle.net/10495/80012256-2958ABSTRACT: Previous studies have demonstrated the existence and expression of genes essential to the process of protein ubiquitination in Giardia intestinalis, indicating that the ubiquitin-proteasome system may be involved in the degradation of proteins during its life cycle of the parasite. In this study, purification of ubiquitin was conducted from protein extracts of G. intestinalis trophozoites. Then, an anti-ubiquitin specific antibody was obtained to standardize an assay for the detection and evaluation of ubiquitination patterns. Finally, HSP90 was identified as an ubiquitinated protein in this protozoan. This post-translational modification could have regulatory effects associated with the functionality of the protein or its turnover to regulate key molecular events during the parasite’s life cycle.RESUMEN: Estudios previos han demostrado la existencia y expresión de genes esenciales para el proceso de ubiquitinación de proteínas en Giardia intestinalis, indicando que el sistema ubiquitina-proteosoma puede estar involucrado en el proceso de degradación de proteínas de este parásito durante su ciclo de vida. En el presente trabajo se realizó la purificación de ubiquitina a partir de extractos proteicos de trofozoítos de G. intestinalis, se produjo un anticuerpo anti-ubiquitina específico que permitió la estandarización de un ensayo para la detección y evaluación de los patrones de ubiquitinación, y se identificó la HSP90 como una proteína ubiquitinada en este protozoario. Esta modificación post-transduccional puede tener efectos regulatorios asociados con la funcionalidad de la proteína o con el recambio para regular eventos moleculares claves durante el ciclo de vida del parásito.application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2HSP90 is a target protein for ubiquitination in Giardia intestinalisHSP90 es una proteína blanco de ubiquitinación en Giardia intestinalisArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRev. Colomb. Cienc. Pecu.115210724Revista Colombiana de Ciencias PecuariasPublicationCC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://bibliotecadigital.udea.edu.co/bitstreams/8afc0b6e-5fef-4062-bc6d-fa66356f97d3/download4afdbb8c545fd630ea7db775da747b2fMD52falseAnonymousREADlicense_textlicense_texttext/html; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstreams/f2850924-9017-418d-ac6c-47a8898aef52/downloadd41d8cd98f00b204e9800998ecf8427eMD53falseAnonymousREADlicense_rdflicense_rdfapplication/rdf+xml; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstreams/03c1c8dd-9a82-471e-ae14-145d2f24711e/downloadd41d8cd98f00b204e9800998ecf8427eMD54falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/e190a4fd-2643-4f33-8fda-98b75195c08f/download8a4605be74aa9ea9d79846c1fba20a33MD55falseAnonymousREADORIGINALChaparro_J_2011_HSP90_target_protein_ubiquitination.pdfChaparro_J_2011_HSP90_target_protein_ubiquitination.pdfArtículo de investigaciónapplication/pdf475816https://bibliotecadigital.udea.edu.co/bitstreams/f3d4d8a8-d3b3-4772-9471-a56a41b3f4c2/downloadf711d25e58172efded88a95912371d54MD51trueAnonymousREADTEXTChaparro_J_2011_HSP90_target_protein_ubiquitination.pdf.txtChaparro_J_2011_HSP90_target_protein_ubiquitination.pdf.txtExtracted texttext/plain37765https://bibliotecadigital.udea.edu.co/bitstreams/d99241e4-fdb3-4e4f-b5ea-ccedc8e8af22/download1250ee797e35285ff8e79b0ee46b7750MD56falseAnonymousREADTHUMBNAILChaparro_J_2011_HSP90_target_protein_ubiquitination.pdf.jpgChaparro_J_2011_HSP90_target_protein_ubiquitination.pdf.jpgGenerated Thumbnailimage/jpeg13532https://bibliotecadigital.udea.edu.co/bitstreams/69b3c360-cd7d-400e-884b-362c346fd08d/download04bdc382f398fce217cc884fc3b721d0MD57falseAnonymousREAD10495/8001oai:bibliotecadigital.udea.edu.co:10495/80012025-03-27 00:46:26.828https://creativecommons.org/licenses/by-nc-sa/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |