Antioxidant and angiotensin I-converting enzyme (ACE) inhibitory peptides of rainbow trout (Oncorhynchus mykiss) viscera hydrolysates subjected to simulated gastrointestinal digestion and intestinal absorption

ABSTRACT: The objective of this work was to evaluate in vitro bioaccessibility, intestinal absorption, antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities of peptides from rainbow trout viscera hydrolysate (H). Rainbow trout Viscera (V) was hydrolyzed by Alcalase® 2.4L and a degree of hydrolysis (DH) of 44.8 ± 2.5% was achieved. Viscera and its hydrolysate were subjected to simulated gastrointestinal digestion (SGID) and intestinal absorption across Caco-2/TC7 cell monolayers. After the hydrolysis with Alcalase® 2.4L and the SGID of V, the species between 60.6 kDa and 13.0 kDa were decreased, causing an increase in species less than 6.51 kDa. The SGID of H did not modify the oxygen radical absorbance capacity (ORAC) or ACE inhibitory values but caused a significant decrease in the hydroxyl radical antioxidant capacity (HORAC) (30.2%). It also produced an increase in ABTS radical cation (ABTS assay) scavenging activity and ferric reducing antioxidant power (FRAP) (9.46% and 20.2%, respectively). Bioactive peptides in H were stable after SGID and they were partially able to cross Caco-2/TC7 cell monolayer, which demonstrates their possible intestinal absorption and their potential to act inside the organism.

• Autores: Zapata Montoya, José Edgar; Vásquez Mazo, Priscilla; Chamorro, Verónica C.; García Fillería, Susan F.; Tironi, Valeria A.
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39524
• Acceso en línea: https://hdl.handle.net/10495/39524
• Palabra clave: Células CACO-2; Caco-2 Cells; Hidrolisados de Proteína; Protein Hydrolysates; Digestión; Digestion; Subproductos de la pesca; Fishery by-products; Bioactive properties; http://aims.fao.org/aos/agrovoc/c_26878; https://id.nlm.nih.gov/mesh/D018938; https://id.nlm.nih.gov/mesh/D011492; https://id.nlm.nih.gov/mesh/D004063
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-nd/4.0/