Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs
ABSTRACT: Escherichia coli populations in the gut increase after weaning, releasing cell wall lipopolysaccharides (LPS), which are potent pro-inflammatory substances. Objective: this study evaluated LPS intake effects on protein expression and activity of intestinal disaccharidases and aminopetidase...
- Autores:
-
Agudelo Trujillo, Jorge Hernán
Parra Suescún, Jaime Eduardo
López Herrera, Albeiro
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/8005
- Acceso en línea:
- http://hdl.handle.net/10495/8005
- Palabra clave:
- Digestive enzymes
Intestinal villi
RT- PCR (Transcripción reversa-PCR)
Swine
Escherichia coli
Lipopolisacarido
Cerdos destetados
Enzimas digestivas
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/2.5/co/
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Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| dc.title.translated.spa.fl_str_mv |
Los lipopolisacáridos de Escherichia coli disminuyen la expresión y la actividad de disacaridasas y aminopeptidasas en lechones destetados |
| title |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| spellingShingle |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs Digestive enzymes Intestinal villi RT- PCR (Transcripción reversa-PCR) Swine Escherichia coli Lipopolisacarido Cerdos destetados Enzimas digestivas |
| title_short |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| title_full |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| title_fullStr |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| title_full_unstemmed |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| title_sort |
Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs |
| dc.creator.fl_str_mv |
Agudelo Trujillo, Jorge Hernán Parra Suescún, Jaime Eduardo López Herrera, Albeiro |
| dc.contributor.author.none.fl_str_mv |
Agudelo Trujillo, Jorge Hernán Parra Suescún, Jaime Eduardo López Herrera, Albeiro |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Ciencias Agrarias -GRICA- |
| dc.subject.none.fl_str_mv |
Digestive enzymes Intestinal villi RT- PCR (Transcripción reversa-PCR) Swine Escherichia coli Lipopolisacarido Cerdos destetados Enzimas digestivas |
| topic |
Digestive enzymes Intestinal villi RT- PCR (Transcripción reversa-PCR) Swine Escherichia coli Lipopolisacarido Cerdos destetados Enzimas digestivas |
| description |
ABSTRACT: Escherichia coli populations in the gut increase after weaning, releasing cell wall lipopolysaccharides (LPS), which are potent pro-inflammatory substances. Objective: this study evaluated LPS intake effects on protein expression and activity of intestinal disaccharidases and aminopetidases. Methods: sixty-four pigs (weaning age: 21 d) were fed a basal diet with added LPS (0.0, 0.3, 0.5, and 1.0 μg LPS/mg feed) during 10 days post-weaning. Pigs were slaughtered on days 1, 5, 7, and 10 post-weaning to assess small intestine molecular expression and enzyme activity. A randomized block design in a 4x4 factorial arrangement was used. Results: intestinal enzyme expression and activity was lower in pigs fed the highest LPS level (p < 0.01). Enzyme values were lowest at day 10 (p < 0.01). The highest gene expression and enzyme activity was observed in duodenum (p < 0.01). Conclusions: data from this study suggest that LPS from E. coli decreases intestinal enzyme expression and activity. |
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2015 |
| dc.date.issued.none.fl_str_mv |
2015 |
| dc.date.accessioned.none.fl_str_mv |
2017-08-23T20:48:12Z |
| dc.date.available.none.fl_str_mv |
2017-08-23T20:48:12Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Parra-Suescún J, Agudelo-Trujillo JH, López-Herrera A. Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs. Rev Colomb Cienc Pecu. 2015;28(1):64-73. |
| dc.identifier.issn.none.fl_str_mv |
0120-0690 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/8005 |
| dc.identifier.eissn.none.fl_str_mv |
2256-2958 |
| identifier_str_mv |
Parra-Suescún J, Agudelo-Trujillo JH, López-Herrera A. Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs. Rev Colomb Cienc Pecu. 2015;28(1):64-73. 0120-0690 2256-2958 |
| url |
http://hdl.handle.net/10495/8005 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
| dc.relation.citationendpage.spa.fl_str_mv |
73 |
| dc.relation.citationissue.spa.fl_str_mv |
1 |
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64 |
| dc.relation.citationvolume.spa.fl_str_mv |
28 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Colombiana de Ciencias Pecuarias |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidad de Antioquia, Facultad de Ciencias Agrarias |
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Medellín, Colombia |
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Universidad de Antioquia |
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Agudelo Trujillo, Jorge HernánParra Suescún, Jaime EduardoLópez Herrera, AlbeiroGrupo de Investigación en Ciencias Agrarias -GRICA-2017-08-23T20:48:12Z2017-08-23T20:48:12Z2015Parra-Suescún J, Agudelo-Trujillo JH, López-Herrera A. Escherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigs. Rev Colomb Cienc Pecu. 2015;28(1):64-73.0120-0690http://hdl.handle.net/10495/80052256-2958ABSTRACT: Escherichia coli populations in the gut increase after weaning, releasing cell wall lipopolysaccharides (LPS), which are potent pro-inflammatory substances. Objective: this study evaluated LPS intake effects on protein expression and activity of intestinal disaccharidases and aminopetidases. Methods: sixty-four pigs (weaning age: 21 d) were fed a basal diet with added LPS (0.0, 0.3, 0.5, and 1.0 μg LPS/mg feed) during 10 days post-weaning. Pigs were slaughtered on days 1, 5, 7, and 10 post-weaning to assess small intestine molecular expression and enzyme activity. A randomized block design in a 4x4 factorial arrangement was used. Results: intestinal enzyme expression and activity was lower in pigs fed the highest LPS level (p < 0.01). Enzyme values were lowest at day 10 (p < 0.01). The highest gene expression and enzyme activity was observed in duodenum (p < 0.01). Conclusions: data from this study suggest that LPS from E. coli decreases intestinal enzyme expression and activity.RESUMEN: la población de Escherichia coli incrementa luego del destete, liberando desde su pared celular lipopolisacáridos (LPS) los cuales son potentes productos pro-inflamatorios. Objetivo: este aminopeptidasas intestinales. Métodos: sesenta y cuatro cerdos (destetados a los 21 de edad) fueron alimentados (durante los 10 días posteriores al destete) con una dieta basal a la cual se le adicionó diferentes niveles de LPS (0,0, 0,3, 0,5 y 1,0 μg LPS/mg alimento). Los cerdos fueron sacrificados los días 1, 5, 7 y 10 posdestete para determinar la expresión molecular y actividad enzimática en el intestino delgado. Se utilizó un diseño de bloques al azar en un arreglo factorial 4x4. Resultados: la expresión y actividad enzimática intestinal fue menor en los cerdos alimentados con el nivel máximo de LPS (p < 0,01). En el día 10 posdestete se observaron las menores expresiones y actividades enzimáticas (p < 0,01), mientras que en el duodeno se observaron los mayores valores (p < 0,01). Conclusión: la información obtenida en este estudio sugiere que el LPS de E. coli disminuye la expresión y actividad enzimática intestinal.COL0009556application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Digestive enzymesIntestinal villiRT- PCR (Transcripción reversa-PCR)SwineEscherichia coliLipopolisacaridoCerdos destetadosEnzimas digestivasEscherichia coli lipopolysaccharides decrease molecular expression and activity of disaccharidases and aminopeptidases in weaned pigsLos lipopolisacáridos de Escherichia coli disminuyen la expresión y la actividad de disacaridasas y aminopeptidasas en lechones destetadosArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRev. Colomb. Cienc. 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