Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2

ABSTRACT : Endocannabinoids are known to mediate practically all reproductive events in mammals; however, little is known about their role in oocyte maturation. Through RT-PCR and immunocytochemistry, this study confirms the presence of CB1 and CB2 cannabinoid receptors in bovine oocytes and shows h...

Full description

Autores:
López Cardona, Ángela Patricia
Sánchez Calabuig, María Jesús
Beltrán Breña, Paula
Agirregoitia, Naiara
Rizos, Dimitrios
Ekaitz Agirregoitia, Marcos
Gutiérrez Adán, Alfonso
Tipo de recurso:
Article of investigation
Fecha de publicación:
2016
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/37534
Acceso en línea:
https://hdl.handle.net/10495/37534
Palabra clave:
Agonistas de Receptores de Cannabinoides
Cannabinoid Receptor Agonists
Bovinos
Cattle
Dronabinol
Oocitos
Oocytes
Desarrollo Embrionario
Embryonic Development
Antagonistas de Aminoácidos Excitadores
Excitatory Amino Acid Antagonists
Regulación Bacteriana de la Expresión Génica
Gene Expression Regulation
Técnicas de Maduración In Vitro de los Oocitos
In Vitro Oocyte Maturation Techniques
Proteína Quinasa 1 Activada por Mitógenos
Mitogen-Activated Protein Kinase 1
Proteína Quinasa 3 Activada por Mitógenos
Mitogen-Activated Protein Kinase 3
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_6d25b35cd30b2bbddf9ea49ad6ccc6bc
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/37534
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
title Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
spellingShingle Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
Agonistas de Receptores de Cannabinoides
Cannabinoid Receptor Agonists
Bovinos
Cattle
Dronabinol
Oocitos
Oocytes
Desarrollo Embrionario
Embryonic Development
Antagonistas de Aminoácidos Excitadores
Excitatory Amino Acid Antagonists
Regulación Bacteriana de la Expresión Génica
Gene Expression Regulation
Técnicas de Maduración In Vitro de los Oocitos
In Vitro Oocyte Maturation Techniques
Proteína Quinasa 1 Activada por Mitógenos
Mitogen-Activated Protein Kinase 1
Proteína Quinasa 3 Activada por Mitógenos
Mitogen-Activated Protein Kinase 3
title_short Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
title_full Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
title_fullStr Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
title_full_unstemmed Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
title_sort Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
dc.creator.fl_str_mv López Cardona, Ángela Patricia
Sánchez Calabuig, María Jesús
Beltrán Breña, Paula
Agirregoitia, Naiara
Rizos, Dimitrios
Ekaitz Agirregoitia, Marcos
Gutiérrez Adán, Alfonso
dc.contributor.author.none.fl_str_mv López Cardona, Ángela Patricia
Sánchez Calabuig, María Jesús
Beltrán Breña, Paula
Agirregoitia, Naiara
Rizos, Dimitrios
Ekaitz Agirregoitia, Marcos
Gutiérrez Adán, Alfonso
dc.contributor.researchgroup.spa.fl_str_mv Biogénesis
dc.subject.decs.none.fl_str_mv Agonistas de Receptores de Cannabinoides
Cannabinoid Receptor Agonists
Bovinos
Cattle
Dronabinol
Oocitos
Oocytes
Desarrollo Embrionario
Embryonic Development
Antagonistas de Aminoácidos Excitadores
Excitatory Amino Acid Antagonists
Regulación Bacteriana de la Expresión Génica
Gene Expression Regulation
Técnicas de Maduración In Vitro de los Oocitos
In Vitro Oocyte Maturation Techniques
Proteína Quinasa 1 Activada por Mitógenos
Mitogen-Activated Protein Kinase 1
Proteína Quinasa 3 Activada por Mitógenos
Mitogen-Activated Protein Kinase 3
topic Agonistas de Receptores de Cannabinoides
Cannabinoid Receptor Agonists
Bovinos
Cattle
Dronabinol
Oocitos
Oocytes
Desarrollo Embrionario
Embryonic Development
Antagonistas de Aminoácidos Excitadores
Excitatory Amino Acid Antagonists
Regulación Bacteriana de la Expresión Génica
Gene Expression Regulation
Técnicas de Maduración In Vitro de los Oocitos
In Vitro Oocyte Maturation Techniques
Proteína Quinasa 1 Activada por Mitógenos
Mitogen-Activated Protein Kinase 1
Proteína Quinasa 3 Activada por Mitógenos
Mitogen-Activated Protein Kinase 3
description ABSTRACT : Endocannabinoids are known to mediate practically all reproductive events in mammals; however, little is known about their role in oocyte maturation. Through RT-PCR and immunocytochemistry, this study confirms the presence of CB1 and CB2 cannabinoid receptors in bovine oocytes and shows how exposure to the exogenous cannabinoids HU-210 and THC during their in vitro maturation (IVM) activates the phosphorylation of AKT and ERK1/2 proteins associated with the resumption of meiosis. Although supplementation with HU-210 or THC during IVM did not increase blastocyst yields, the expression of interferon tau (IFNτ) and gap junction alpha-1 protein (GJA1) was enhanced at the blastocyst stage. Our data suggest that cannabinoid agonists may be useful IVM supplements as their presence during oocyte maturation upregulates the expression in blastocysts of key genes for embryo quality.
publishDate 2016
dc.date.issued.none.fl_str_mv 2016
dc.date.accessioned.none.fl_str_mv 2023-12-09T16:19:35Z
dc.date.available.none.fl_str_mv 2023-12-09T16:19:35Z
dc.type.spa.fl_str_mv Artículo de investigación
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.citation.spa.fl_str_mv López-Cardona AP, Sánchez-Calabuig MJ, Beltran-Breña P, Agirregoitia N, Rizos D, Agirregoitia E, Gutiérrez-Adán A. Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2. Reproduction. 2016 Dec;152(6):603-612. doi: 10.1530/REP-16-0199. PMID: 27798282.
dc.identifier.issn.none.fl_str_mv 1470-1626
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/37534
dc.identifier.doi.none.fl_str_mv 10.1530/REP-16-0199
dc.identifier.eissn.none.fl_str_mv 1741-7899
identifier_str_mv López-Cardona AP, Sánchez-Calabuig MJ, Beltran-Breña P, Agirregoitia N, Rizos D, Agirregoitia E, Gutiérrez-Adán A. Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2. Reproduction. 2016 Dec;152(6):603-612. doi: 10.1530/REP-16-0199. PMID: 27798282.
1470-1626
10.1530/REP-16-0199
1741-7899
url https://hdl.handle.net/10495/37534
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Reproduction
dc.relation.citationendpage.spa.fl_str_mv 612
dc.relation.citationissue.spa.fl_str_mv 6
dc.relation.citationstartpage.spa.fl_str_mv 603
dc.relation.citationvolume.spa.fl_str_mv 152
dc.relation.ispartofjournal.spa.fl_str_mv Reproduction
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/co/
https://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 10
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Society for Reproduction and Fertility
dc.publisher.place.spa.fl_str_mv Bristol, Inglaterra
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstreams/57d9e9ab-d15d-4e6f-bfe1-0e10adb79583/download
https://bibliotecadigital.udea.edu.co/bitstreams/2c22d911-2df6-41d0-9a4a-4edace2270ef/download
https://bibliotecadigital.udea.edu.co/bitstreams/f8a562b3-f934-403a-94ee-73bb23050e4b/download
https://bibliotecadigital.udea.edu.co/bitstreams/74020fff-6a8a-4945-a1da-e89b55a7e135/download
https://bibliotecadigital.udea.edu.co/bitstreams/efa995ab-da25-4972-a8cd-8ed2afb4bb0b/download
bitstream.checksum.fl_str_mv 12753b510d65284cce31fa47f8b1e7ed
1646d1f6b96dbbbc38035efc9239ac9c
8a4605be74aa9ea9d79846c1fba20a33
9c3a1facada9a8369356f1b3a1b3f1df
7f7049e3e264479a61f93f78e0a347d2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional de la Universidad de Antioquia
repository.mail.fl_str_mv aplicacionbibliotecadigitalbiblioteca@udea.edu.co
_version_ 1851052595887472640
spelling López Cardona, Ángela PatriciaSánchez Calabuig, María JesúsBeltrán Breña, PaulaAgirregoitia, NaiaraRizos, DimitriosEkaitz Agirregoitia, MarcosGutiérrez Adán, AlfonsoBiogénesis2023-12-09T16:19:35Z2023-12-09T16:19:35Z2016López-Cardona AP, Sánchez-Calabuig MJ, Beltran-Breña P, Agirregoitia N, Rizos D, Agirregoitia E, Gutiérrez-Adán A. Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2. Reproduction. 2016 Dec;152(6):603-612. doi: 10.1530/REP-16-0199. PMID: 27798282.1470-1626https://hdl.handle.net/10495/3753410.1530/REP-16-01991741-7899ABSTRACT : Endocannabinoids are known to mediate practically all reproductive events in mammals; however, little is known about their role in oocyte maturation. Through RT-PCR and immunocytochemistry, this study confirms the presence of CB1 and CB2 cannabinoid receptors in bovine oocytes and shows how exposure to the exogenous cannabinoids HU-210 and THC during their in vitro maturation (IVM) activates the phosphorylation of AKT and ERK1/2 proteins associated with the resumption of meiosis. Although supplementation with HU-210 or THC during IVM did not increase blastocyst yields, the expression of interferon tau (IFNτ) and gap junction alpha-1 protein (GJA1) was enhanced at the blastocyst stage. Our data suggest that cannabinoid agonists may be useful IVM supplements as their presence during oocyte maturation upregulates the expression in blastocysts of key genes for embryo quality.Universidad del País VascoColombia. Ministerio de Ciencia, Tecnología e InnovaciónEspaña. Secretaría de Estado de Investigación, Desarrollo e InnovaciónCOL006656110application/pdfengSociety for Reproduction and FertilityBristol, Inglaterrahttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2Artículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAgonistas de Receptores de CannabinoidesCannabinoid Receptor AgonistsBovinosCattleDronabinolOocitosOocytesDesarrollo EmbrionarioEmbryonic DevelopmentAntagonistas de Aminoácidos ExcitadoresExcitatory Amino Acid AntagonistsRegulación Bacteriana de la Expresión GénicaGene Expression RegulationTécnicas de Maduración In Vitro de los OocitosIn Vitro Oocyte Maturation TechniquesProteína Quinasa 1 Activada por MitógenosMitogen-Activated Protein Kinase 1Proteína Quinasa 3 Activada por MitógenosMitogen-Activated Protein Kinase 3Reproduction6126603152Reproduction501100003451RoR:03fd5ne08RoR:000xsnr85Fondo Francisco José de CaldasGIU14/26512/2010A G A: AGL2012- 39652 and D R: AGL2012-37510512/2010.PublicationORIGINALLopezAngela_2016_BovineOocyteMaturation.pdfLopezAngela_2016_BovineOocyteMaturation.pdfArtículo de investigaciónapplication/pdf2877363https://bibliotecadigital.udea.edu.co/bitstreams/57d9e9ab-d15d-4e6f-bfe1-0e10adb79583/download12753b510d65284cce31fa47f8b1e7edMD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstreams/2c22d911-2df6-41d0-9a4a-4edace2270ef/download1646d1f6b96dbbbc38035efc9239ac9cMD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/f8a562b3-f934-403a-94ee-73bb23050e4b/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTLopezAngela_2016_BovineOocyteMaturation.pdf.txtLopezAngela_2016_BovineOocyteMaturation.pdf.txtExtracted texttext/plain56620https://bibliotecadigital.udea.edu.co/bitstreams/74020fff-6a8a-4945-a1da-e89b55a7e135/download9c3a1facada9a8369356f1b3a1b3f1dfMD54falseAnonymousREADTHUMBNAILLopezAngela_2016_BovineOocyteMaturation.pdf.jpgLopezAngela_2016_BovineOocyteMaturation.pdf.jpgGenerated Thumbnailimage/jpeg15545https://bibliotecadigital.udea.edu.co/bitstreams/efa995ab-da25-4972-a8cd-8ed2afb4bb0b/download7f7049e3e264479a61f93f78e0a347d2MD55falseAnonymousREAD10495/37534oai:bibliotecadigital.udea.edu.co:10495/375342025-03-27 00:48:26.512http://creativecommons.org/licenses/by/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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

Publicaciones similares