Low frequency of IL-10+ B cells in patients with atherosclerosis is related with inflammatory condition

ABSTRACT:Background and aims: B cells involvement in animal models of atherosclerosis has been unequivocally established. However, the role of these cells in patients with atherosclerosis is almost unknown. Besides the production of antibodies, B cells can also exhibit regulatory functions mainly through IL-10. Here, we characterized human B cell subsets, their production of IL-10 in patients with atherosclerosis and their potential association with inflammation. Methods: Patients with confirmed atherosclerotic events and controls with low cardiovascular risk were included. B cells subsets were determined in mononuclear cells (PBMC) using flow cytometry. PBMC were cultured ex vivo (5 h) and in vitro (48 h) to determine IL-10+ B cells and in some cases TNF-α+ and IFN-γ+ CD4+ T cells. The inflammatory state of the participants was determined through high sensitivity C reactive protein levels. Results: Increase in percentage and number of plasmablasts was observed in patients with atherosclerosis compared with controls. A decreased frequency of IL-10+ B cells was observed in patients, both in ex vivo and in vitro cultures. This decrease was detected in transitional, memory, and plasmablast subsets. Interestingly, the reduction of IL-10+ B cells negatively and significantly correlated with the inflammatory condition of the studied subjects and associated with an increased frequency of TNF-α+ and IFN-γ+ CD4+ T cells. The blockade of IL-10R did not show further effect in T cells activation. Conclusions: There is an association between the inflammatory state and a reduction of IL-10+ B cells that could contribute to the development of atherosclerosis.

• Autores: Rincón Arévalo, Héctor Julián; Quintero Hurtado, Julio César; Fortich Hoyos, Fernando Manuel; Rojas López, Mauricio; Vásquez Duque, Gloria María; Castaño Monsalve, Diana María; Yassin Noreña, Lina María
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2020
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/42300
• Acceso en línea: https://hdl.handle.net/10495/42300
• Palabra clave: Aterosclerosis; Atherosclerosis; Subgrupos de Linfocitos B; B-Lymphocyte Subsets; Linfocitos B; B-Lymphocytes; Proteína C-Reactiva; C-Reactive Protein; Colesterol; Cholesterol; Sistema Cardiovascular; Cardiovascular System; Interleucina-10; Interleukin-10; Enfermedades del Sistema Inmune; Immune System Diseases; Sistema Inmunológico; Immune System; Linfocitos B Reguladores; B-Lymphocytes, Regulatory; Inflamación; Inflammation; https://id.nlm.nih.gov/mesh/D050197; https://id.nlm.nih.gov/mesh/D016175; https://id.nlm.nih.gov/mesh/D001402; https://id.nlm.nih.gov/mesh/D002097; https://id.nlm.nih.gov/mesh/D002784; https://id.nlm.nih.gov/mesh/D002319; https://id.nlm.nih.gov/mesh/D016753; https://id.nlm.nih.gov/mesh/D007154; https://id.nlm.nih.gov/mesh/D007107; https://id.nlm.nih.gov/mesh/D060151; https://id.nlm.nih.gov/mesh/D007249
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-nd/2.5/co/