Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular
ABSTRACT: Tissue engineering seeks for the possibility of the human body to regenerate itself by the creation of an artificial environment appropriate for cells. One of the approaches has been the development of scaffolds will allow the cells to grow and proliferate. In this research PVAchitosan sca...
- Autores:
-
Almonacid Suárez, Ana María
Londoño López, Marta Elena
Gartner, Carmiña
Echeverri, Claudia Elena
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/5794
- Acceso en línea:
- http://hdl.handle.net/10495/5794
- Palabra clave:
- Ingeniería de tejidos
Tissue Engineering
Proliferación Celular
Cell Proliferation
Alcohol polivinílico
Polyvinyl Alcohol
Cultivo de celulas
Cell culture
Tejidos humanos
Regeneración de tejidos
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| title |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| spellingShingle |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular Ingeniería de tejidos Tissue Engineering Proliferación Celular Cell Proliferation Alcohol polivinílico Polyvinyl Alcohol Cultivo de celulas Cell culture Tejidos humanos Regeneración de tejidos |
| title_short |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| title_full |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| title_fullStr |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| title_full_unstemmed |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| title_sort |
Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular |
| dc.creator.fl_str_mv |
Almonacid Suárez, Ana María Londoño López, Marta Elena Gartner, Carmiña Echeverri, Claudia Elena |
| dc.contributor.author.none.fl_str_mv |
Almonacid Suárez, Ana María Londoño López, Marta Elena Gartner, Carmiña Echeverri, Claudia Elena |
| dc.contributor.researchgroup.spa.fl_str_mv |
Ciencia de los Materiales |
| dc.subject.decs.none.fl_str_mv |
Ingeniería de tejidos Tissue Engineering Proliferación Celular Cell Proliferation Alcohol polivinílico Polyvinyl Alcohol |
| topic |
Ingeniería de tejidos Tissue Engineering Proliferación Celular Cell Proliferation Alcohol polivinílico Polyvinyl Alcohol Cultivo de celulas Cell culture Tejidos humanos Regeneración de tejidos |
| dc.subject.lemb.none.fl_str_mv |
Cultivo de celulas Cell culture |
| dc.subject.proposal.spa.fl_str_mv |
Tejidos humanos Regeneración de tejidos |
| description |
ABSTRACT: Tissue engineering seeks for the possibility of the human body to regenerate itself by the creation of an artificial environment appropriate for cells. One of the approaches has been the development of scaffolds will allow the cells to grow and proliferate. In this research PVAchitosan scaffolds were developed using gas foaming/particulate leaching technique. Mechanical compression tests, scanning electron microscopy (SEM), infrared spectroscopy (FTIR) and a preliminary cell culture with Chinese hamster ovary cell line (CHO) was performed. Cell adhesion was evaluated by Giemsa stain. PVA-chitosan scaffolds showed mechanical and structural stability and an adequate porosity and interconnectivity. Pore sizes were larger than 10μm. Finally, Giemsa results for the preliminary cell culture revealed cells adhesion within the scaffold. |
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2014 |
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2014 |
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2017-01-07T15:00:49Z |
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2017-01-07T15:00:49Z |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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A. M. Almonacid, M. E. Londoño, C. Gartner and C. E. Echeverri, “Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular”, Revista Colombiana de Materiales, no. 5, pp. 1-7, 2014. |
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2256-1013 |
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http://hdl.handle.net/10495/5794 |
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A. M. Almonacid, M. E. Londoño, C. Gartner and C. E. Echeverri, “Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular”, Revista Colombiana de Materiales, no. 5, pp. 1-7, 2014. 2256-1013 |
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http://hdl.handle.net/10495/5794 |
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spa |
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spa |
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Revista Colombiana de Materiales |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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Universidad de Antioquia, Facultad de Ingeniería |
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Medellín, Colombia |
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Universidad de Antioquia |
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Almonacid Suárez, Ana MaríaLondoño López, Marta ElenaGartner, CarmiñaEcheverri, Claudia ElenaCiencia de los Materiales2017-01-07T15:00:49Z2017-01-07T15:00:49Z2014A. M. Almonacid, M. E. Londoño, C. Gartner and C. E. Echeverri, “Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celular”, Revista Colombiana de Materiales, no. 5, pp. 1-7, 2014.2256-1013http://hdl.handle.net/10495/5794ABSTRACT: Tissue engineering seeks for the possibility of the human body to regenerate itself by the creation of an artificial environment appropriate for cells. One of the approaches has been the development of scaffolds will allow the cells to grow and proliferate. In this research PVAchitosan scaffolds were developed using gas foaming/particulate leaching technique. Mechanical compression tests, scanning electron microscopy (SEM), infrared spectroscopy (FTIR) and a preliminary cell culture with Chinese hamster ovary cell line (CHO) was performed. Cell adhesion was evaluated by Giemsa stain. PVA-chitosan scaffolds showed mechanical and structural stability and an adequate porosity and interconnectivity. Pore sizes were larger than 10μm. Finally, Giemsa results for the preliminary cell culture revealed cells adhesion within the scaffold.RESUMEN: La ingeniería de tejidos busca reemplazar o crear un ambiente propicio para que los tejidos del cuerpo humano puedan regenerarse. Una de las soluciones planteadas para lograr este objetivo es la creación de soportes o matrices para facilitar el crecimiento y proliferación celular. En el presente trabajo se desarrollaron matrices para cultivo celular a partir de alcohol polivinílico (PVA) y quitosano mediante la técnica de espumado/lixiviado. Se realizaron pruebas mecánicas, espectroscopia infrarroja (FTIR), microscopia electrónica de barrido (SEM) y finalmente, se efectuó un cultivo preliminar con células de hámster chino (CHO) donde se evaluó la adhesión celular por medio de la tinción Giemsa. Las microscopías exhibieron alta porosidad e interconectada con un tamaño de poro mayor a 10μm. Las matrices de PVA y quitosano obtenidas son estables mecánica y estructuralmente. Adicionalmente, los resultados de la tinción Giemsa en el cultivo preliminar revelaron células adheridas y embebidas en la matriz.application/pdfspaUniversidad de Antioquia, Facultad de IngenieríaMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/4.0/https://creativecommons.org/licenses/by-nc-sa/2.5/co/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Obtención y caracterización de soportes de alcohol polivinílico y quitosano para cultivo celularArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionIngeniería de tejidosTissue EngineeringProliferación CelularCell ProliferationAlcohol polivinílicoPolyvinyl AlcoholCultivo de celulasCell cultureTejidos humanosRegeneración de tejidos751Revista Colombiana de MaterialesPublicationORIGINALAlmonacidAna_2014_AlcoholPolivinilicoQuitosano.pdfAlmonacidAna_2014_AlcoholPolivinilicoQuitosano.pdfArtículo de investigaciónapplication/pdf504580https://bibliotecadigital.udea.edu.co/bitstreams/45ebeba7-f615-4915-ba84-5756b000a226/download402ba1257f1c83e5c2a24ba36707a328MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; 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