In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles
ABSTRACT: Objectives: To evaluate in vitro essential oils, enriched fractions and terpenes against clinical isolates of C. auris with different antifungal susceptibility profiles. Materials & Methods: Four fluconazole-resistant C. glabrata isolates were used. FLZ-NLCs were prepared using the ult...
- Autores:
-
Zapata Zapata, Carolina
Mesa Arango, Ana Cecilia
Loaiza Oliva, Manuela
Gómez Velásquez, Juan Carlos
Torcoroma García, Liliana
Stashenko, Elena
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/45055
- Acceso en línea:
- https://hdl.handle.net/10495/45055
- Palabra clave:
- Candida auris
Antifúngicos
Antifungal Agents
Aceite esencial
Assential oils
http://aims.fao.org/aos/agrovoc/c_2669
https://id.nlm.nih.gov/mesh/D000088063
https://id.nlm.nih.gov/mesh/D000935
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| title |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| spellingShingle |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles Candida auris Antifúngicos Antifungal Agents Aceite esencial Assential oils http://aims.fao.org/aos/agrovoc/c_2669 https://id.nlm.nih.gov/mesh/D000088063 https://id.nlm.nih.gov/mesh/D000935 |
| title_short |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| title_full |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| title_fullStr |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| title_full_unstemmed |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| title_sort |
In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility Profiles |
| dc.creator.fl_str_mv |
Zapata Zapata, Carolina Mesa Arango, Ana Cecilia Loaiza Oliva, Manuela Gómez Velásquez, Juan Carlos Torcoroma García, Liliana Stashenko, Elena |
| dc.contributor.author.none.fl_str_mv |
Zapata Zapata, Carolina Mesa Arango, Ana Cecilia Loaiza Oliva, Manuela Gómez Velásquez, Juan Carlos Torcoroma García, Liliana Stashenko, Elena |
| dc.contributor.researchgroup.spa.fl_str_mv |
GRID - Grupo de Investigación Dermatológica Grupo Académico de Epidemiología Clínica |
| dc.subject.decs.none.fl_str_mv |
Candida auris Antifúngicos Antifungal Agents |
| topic |
Candida auris Antifúngicos Antifungal Agents Aceite esencial Assential oils http://aims.fao.org/aos/agrovoc/c_2669 https://id.nlm.nih.gov/mesh/D000088063 https://id.nlm.nih.gov/mesh/D000935 |
| dc.subject.agrovoc.none.fl_str_mv |
Aceite esencial Assential oils |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_2669 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D000088063 https://id.nlm.nih.gov/mesh/D000935 |
| description |
ABSTRACT: Objectives: To evaluate in vitro essential oils, enriched fractions and terpenes against clinical isolates of C. auris with different antifungal susceptibility profiles. Materials & Methods: Four fluconazole-resistant C. glabrata isolates were used. FLZ-NLCs were prepared using the ultrasound technique. Each fluconazole-resistant C. glabrata isolate was exposed to fluconazole (alone and in the form of FLZ-NLCs) and placebo (NLCs without fluconazole (FLZ)) for 20 h at 37 ◦C and the total RNA extracted. Real-time PCRs were performed to assess the probable alternations in ATP-binding cassette transporter genes. Results: Under the FLZ-NLCs treated condition, no significant alteration was ob-served in the expression of CgCDR1, CgCDR2 and CgSNQ2 genes although the genes wereover-expressed when exposed to fluconazole. Conclusions: It is highly suggested that nanostructure lipid carrier may prevent drug detection by transporter’s proteins avoiding the drug pumping out. As such, the developed formulation may be fundamentally beneficial for the treatment of candidiasis caused by C. glabrata. Acknowledgments: The authors thank funding from the Ministry of Science, Tech-nology and Innovation, the Ministry of Education, the Ministry of Industry and Commerce. |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2025-02-19T21:58:20Z |
| dc.date.available.none.fl_str_mv |
2025-02-19T21:58:20Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
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https://purl.org/redcol/resource_type/ARTDIV |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/45055 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/jof7110916 |
| dc.identifier.eissn.none.fl_str_mv |
2309-608X |
| url |
https://hdl.handle.net/10495/45055 |
| identifier_str_mv |
10.3390/jof7110916 2309-608X |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
J. Fungi. |
| dc.relation.citationendpage.spa.fl_str_mv |
273 |
| dc.relation.citationissue.spa.fl_str_mv |
11 |
| dc.relation.citationstartpage.spa.fl_str_mv |
272 |
| dc.relation.citationvolume.spa.fl_str_mv |
7 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Journal of Fungi |
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http://creativecommons.org/licenses/by/2.5/co/ |
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https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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2 páginas |
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application/pdf |
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MDPI |
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Basilea, Suiza |
| institution |
Universidad de Antioquia |
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Zapata Zapata, CarolinaMesa Arango, Ana CeciliaLoaiza Oliva, ManuelaGómez Velásquez, Juan CarlosTorcoroma García, LilianaStashenko, ElenaGRID - Grupo de Investigación DermatológicaGrupo Académico de Epidemiología Clínica2025-02-19T21:58:20Z2025-02-19T21:58:20Z2021https://hdl.handle.net/10495/4505510.3390/jof71109162309-608XABSTRACT: Objectives: To evaluate in vitro essential oils, enriched fractions and terpenes against clinical isolates of C. auris with different antifungal susceptibility profiles. Materials & Methods: Four fluconazole-resistant C. glabrata isolates were used. FLZ-NLCs were prepared using the ultrasound technique. Each fluconazole-resistant C. glabrata isolate was exposed to fluconazole (alone and in the form of FLZ-NLCs) and placebo (NLCs without fluconazole (FLZ)) for 20 h at 37 ◦C and the total RNA extracted. Real-time PCRs were performed to assess the probable alternations in ATP-binding cassette transporter genes. Results: Under the FLZ-NLCs treated condition, no significant alteration was ob-served in the expression of CgCDR1, CgCDR2 and CgSNQ2 genes although the genes wereover-expressed when exposed to fluconazole. Conclusions: It is highly suggested that nanostructure lipid carrier may prevent drug detection by transporter’s proteins avoiding the drug pumping out. As such, the developed formulation may be fundamentally beneficial for the treatment of candidiasis caused by C. glabrata. Acknowledgments: The authors thank funding from the Ministry of Science, Tech-nology and Innovation, the Ministry of Education, the Ministry of Industry and Commerce.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MinCienciasColombia. Ministerio de Educación Nacional - MinEducaciónColombia. Ministerio de Comercio, Industria y Turismo - MinCITICETEX (Instituto Colombiano de Crédito Educativo y Estudios Técnicos en el Exterior)COL0050839COL00071212 páginasapplication/pdfengMDPIBasilea, Suizahttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2In Vitro Evaluation of Essential Oils, Fractions and Terpenes against Clinical Isolates of Candida auris with Different Antifungal Susceptibility ProfilesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTDIVhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCandida aurisAntifúngicosAntifungal AgentsAceite esencialAssential oilshttp://aims.fao.org/aos/agrovoc/c_2669https://id.nlm.nih.gov/mesh/D000088063https://id.nlm.nih.gov/mesh/D000935J. 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