New insights into cell cycle profile of Paracoccidioides brasiliensis
ABSTRACT: The present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environ- mental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentia...
- Autores:
-
Cano Restrepo, Luz Elena
Restrepo Moreno, Ángela
Ramalho Almeida, Agostinho Joao
Carmona, Jenifer Andrea
Martins, Margarida
Leão, Cecília
Santos Rodrigues, Fernando José
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2006
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/43508
- Acceso en línea:
- https://hdl.handle.net/10495/43508
- Palabra clave:
- Paracoccidioides
Citometría de Flujo
Flow cytometry
Puntos de Control del Ciclo Celular
Cell Cycle Checkpoints
Análisis de Varianza
Analysis of Variance
Benomilo
Benomyl
ADN de Hongos - metabolismo
DNA, Fungal - metabolism
Diaminas
Diamines
Fungicidas Industriales
Fungicides, Industrial
https://id.nlm.nih.gov/mesh/D010228
https://id.nlm.nih.gov/mesh/D005434
https://id.nlm.nih.gov/mesh/D059447
https://id.nlm.nih.gov/mesh/D000704
https://id.nlm.nih.gov/mesh/D004271
https://id.nlm.nih.gov/mesh/D003959
https://id.nlm.nih.gov/mesh/D005659
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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| dc.title.spa.fl_str_mv |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| title |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| spellingShingle |
New insights into cell cycle profile of Paracoccidioides brasiliensis Paracoccidioides Citometría de Flujo Flow cytometry Puntos de Control del Ciclo Celular Cell Cycle Checkpoints Análisis de Varianza Analysis of Variance Benomilo Benomyl ADN de Hongos - metabolismo DNA, Fungal - metabolism Diaminas Diamines Fungicidas Industriales Fungicides, Industrial https://id.nlm.nih.gov/mesh/D010228 https://id.nlm.nih.gov/mesh/D005434 https://id.nlm.nih.gov/mesh/D059447 https://id.nlm.nih.gov/mesh/D000704 https://id.nlm.nih.gov/mesh/D004271 https://id.nlm.nih.gov/mesh/D003959 https://id.nlm.nih.gov/mesh/D005659 |
| title_short |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| title_full |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| title_fullStr |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| title_full_unstemmed |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| title_sort |
New insights into cell cycle profile of Paracoccidioides brasiliensis |
| dc.creator.fl_str_mv |
Cano Restrepo, Luz Elena Restrepo Moreno, Ángela Ramalho Almeida, Agostinho Joao Carmona, Jenifer Andrea Martins, Margarida Leão, Cecília Santos Rodrigues, Fernando José |
| dc.contributor.author.none.fl_str_mv |
Cano Restrepo, Luz Elena Restrepo Moreno, Ángela Ramalho Almeida, Agostinho Joao Carmona, Jenifer Andrea Martins, Margarida Leão, Cecília Santos Rodrigues, Fernando José |
| dc.contributor.researchgroup.spa.fl_str_mv |
Micología Médica y Experimental Microbiología Molecular |
| dc.subject.decs.none.fl_str_mv |
Paracoccidioides Citometría de Flujo Flow cytometry Puntos de Control del Ciclo Celular Cell Cycle Checkpoints Análisis de Varianza Analysis of Variance Benomilo Benomyl ADN de Hongos - metabolismo DNA, Fungal - metabolism Diaminas Diamines Fungicidas Industriales Fungicides, Industrial |
| topic |
Paracoccidioides Citometría de Flujo Flow cytometry Puntos de Control del Ciclo Celular Cell Cycle Checkpoints Análisis de Varianza Analysis of Variance Benomilo Benomyl ADN de Hongos - metabolismo DNA, Fungal - metabolism Diaminas Diamines Fungicidas Industriales Fungicides, Industrial https://id.nlm.nih.gov/mesh/D010228 https://id.nlm.nih.gov/mesh/D005434 https://id.nlm.nih.gov/mesh/D059447 https://id.nlm.nih.gov/mesh/D000704 https://id.nlm.nih.gov/mesh/D004271 https://id.nlm.nih.gov/mesh/D003959 https://id.nlm.nih.gov/mesh/D005659 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D010228 https://id.nlm.nih.gov/mesh/D005434 https://id.nlm.nih.gov/mesh/D059447 https://id.nlm.nih.gov/mesh/D000704 https://id.nlm.nih.gov/mesh/D004271 https://id.nlm.nih.gov/mesh/D003959 https://id.nlm.nih.gov/mesh/D005659 |
| description |
ABSTRACT: The present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environ- mental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poordeWned or rich-complex nutritional environments showed an increased percentage of daughter cells in accordance with the fungus’ multiple budding and high growth rate. During the stationary growth-phase cell cycle progression in rich-complex medium was characterized by an accumulation of cells with higher DNA content or pseudohyphae-like structures, whereas in poor-deWned medium arrested cells mainly displayed two DNA contents. Furthermore, the fungicide benomyl induced an arrest of the cell cycle with accumulation of cells presenting high and varying DNA contents, consistent with this fungus’ unique pattern of cellular division. Altogether, our Wndings seem to indicate that P. brasiliensis may possess alternative control mechanisms during cell growth to manage multiple budding and its multinucleate nature. |
| publishDate |
2006 |
| dc.date.issued.none.fl_str_mv |
2006 |
| dc.date.accessioned.none.fl_str_mv |
2024-11-15T17:11:27Z |
| dc.date.available.none.fl_str_mv |
2024-11-15T17:11:27Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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| dc.identifier.citation.spa.fl_str_mv |
Almeida AJ, Martins M, Carmona JA, Cano LE, Restrepo A, Leão C, Rodrigues F. New insights into the cell cycle profile of Paracoccidioides brasiliensis. Fungal Genet Biol. 2006 Jun;43(6):401-9. doi: 10.1016/j.fgb.2006.01.012. |
| dc.identifier.issn.none.fl_str_mv |
1087-1845 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/43508 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.fgb.2006.01.012 |
| dc.identifier.eissn.none.fl_str_mv |
1096-0937 |
| identifier_str_mv |
Almeida AJ, Martins M, Carmona JA, Cano LE, Restrepo A, Leão C, Rodrigues F. New insights into the cell cycle profile of Paracoccidioides brasiliensis. Fungal Genet Biol. 2006 Jun;43(6):401-9. doi: 10.1016/j.fgb.2006.01.012. 1087-1845 10.1016/j.fgb.2006.01.012 1096-0937 |
| url |
https://hdl.handle.net/10495/43508 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Fungal Genet. Biol. |
| dc.relation.citationendpage.spa.fl_str_mv |
409 |
| dc.relation.citationissue.spa.fl_str_mv |
6 |
| dc.relation.citationstartpage.spa.fl_str_mv |
401 |
| dc.relation.citationvolume.spa.fl_str_mv |
43 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Fungal Genetics and Biology |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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9 páginas |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Elsevier Academic Press |
| dc.publisher.place.spa.fl_str_mv |
Orlando, Estados Unidos |
| institution |
Universidad de Antioquia |
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Cano Restrepo, Luz ElenaRestrepo Moreno, ÁngelaRamalho Almeida, Agostinho JoaoCarmona, Jenifer AndreaMartins, MargaridaLeão, CecíliaSantos Rodrigues, Fernando JoséMicología Médica y ExperimentalMicrobiología Molecular2024-11-15T17:11:27Z2024-11-15T17:11:27Z2006Almeida AJ, Martins M, Carmona JA, Cano LE, Restrepo A, Leão C, Rodrigues F. New insights into the cell cycle profile of Paracoccidioides brasiliensis. Fungal Genet Biol. 2006 Jun;43(6):401-9. doi: 10.1016/j.fgb.2006.01.012.1087-1845https://hdl.handle.net/10495/4350810.1016/j.fgb.2006.01.0121096-0937ABSTRACT: The present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environ- mental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poordeWned or rich-complex nutritional environments showed an increased percentage of daughter cells in accordance with the fungus’ multiple budding and high growth rate. During the stationary growth-phase cell cycle progression in rich-complex medium was characterized by an accumulation of cells with higher DNA content or pseudohyphae-like structures, whereas in poor-deWned medium arrested cells mainly displayed two DNA contents. Furthermore, the fungicide benomyl induced an arrest of the cell cycle with accumulation of cells presenting high and varying DNA contents, consistent with this fungus’ unique pattern of cellular division. Altogether, our Wndings seem to indicate that P. brasiliensis may possess alternative control mechanisms during cell growth to manage multiple budding and its multinucleate nature.Fundação para a Ciência e TecnologiaCOL0013746COL00137099 páginasapplication/pdfengElsevierAcademic PressOrlando, Estados Unidoshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2New insights into cell cycle profile of Paracoccidioides brasiliensisArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionParacoccidioidesCitometría de FlujoFlow cytometryPuntos de Control del Ciclo CelularCell Cycle CheckpointsAnálisis de VarianzaAnalysis of VarianceBenomiloBenomylADN de Hongos - metabolismoDNA, Fungal - metabolismDiaminasDiaminesFungicidas IndustrialesFungicides, Industrialhttps://id.nlm.nih.gov/mesh/D010228https://id.nlm.nih.gov/mesh/D005434https://id.nlm.nih.gov/mesh/D059447https://id.nlm.nih.gov/mesh/D000704https://id.nlm.nih.gov/mesh/D004271https://id.nlm.nih.gov/mesh/D003959https://id.nlm.nih.gov/mesh/D005659Fungal Genet. 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