Proinflammatory Differentiation of Macrophages Through Microparticles That Form Immune Complexes Leads to T- and B-Cell Activation in Systemic Autoimmune Diseases

ABSTRACT: Patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) demonstrate increased circulating microparticles (MP). These vesicles, primarily those that form immune complexes (MP-IC), may activate monocytes. We evaluated the effect of MP and MP-IC in the differentiation of monocytes to macrophages (monocyte-derived macrophages; MDM) and for consequences in autologous lymphocyte activation. Monocytes from healthy controls (HC) and patients with RA and SLE that differentiated into MDM in the presence of MP-IC showed a proinflammatory (M1-like) profile, which was more evident using MP-IC from patients with RA than those from patients with SLE. Notably, MDM from HC and patients with RA that differentiated with MP-IC were more prone to M1-like profile than those from patients with SLE. In HC and patients with RA, monocyte differentiation using MP-IC decreased the frequency of MDM that bound/internalized latex beads. The M1-like profile did not completely revert following IL-4 treatment. The effect of M1-like MDM on T lymphocytes stimulated with phytohemagglutinin was further evaluated. MDM differentiated with MP enhanced the proliferation of T cells obtained from patients with RA compared with those differentiated with MP-IC or without vesicles. Neither MP nor MP-IC induced interferon (IFN)-γ+ and tumor necrosis factor (TNF)-α+ T cells in patients with RA. Conversely, unlike MDM differentiated with or without MP, MP-IC enhanced the proliferation and increased the frequencies of IFN-γ+CD4+ T, TNF-α+CD4+ T, and IFN-γ+CD8+ T cells in patients with SLE. The co-culture of B cells with MDM obtained from patients with RA and SLE and differentiated with MP-IC increased the expression of B-cell activation markers and prevented B lymphocyte death. Strikingly, only for patients with SLE, these responses seemed to be associated with a significant increase in B-cell activating factor levels, high plasmablast frequency and immunoglobulin production. These results showed that MP-IC from patients with systemic autoimmune diseases favored the polarization of MDM into a proinflammatory profile that promotes T-cell activation, and additionally induced B-cell activation and survival. Therefore, the effect of MP-IC in mononuclear phagocytes may be an important factor for modulating adaptive responses in systemic autoimmune diseases.

• Autores: Burbano Arciniegas, Catalina; Villar Vesga, Juan Manuel; Vásquez Duque, Gloria María; Muñoz Vahos, Carlos; Rojas López, Mauricio; Castaño Monsalve, Diana María
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2019
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/43135
• Acceso en línea: https://hdl.handle.net/10495/43135
• Palabra clave: Enfermedades Autoinmunes; Autoimmune Diseases; Linfocitos B; B-Lymphocytes; Artritis Reumatoide; Arthritis, Rheumatoid; Biomarcadores; Biomarkers; Diferenciación Celular; Cell Differentiation; Micropartículas Derivadas de Células - metabolismo; Cell-Derived Microparticles - metabolism; Técnicas de Cocultivo; Coculture Techniques; Citocinas - metabolismo; Cytokines - metabolism; Mediadores de Inflamación; Inflammation Mediators; Activación de Linfocitos; Lymphocyte Activation; Macrófagos; Macrophages; Fagocitos; Phagocytes; Linfocitos T; T-Lymphocytes; https://id.nlm.nih.gov/mesh/D001327; https://id.nlm.nih.gov/mesh/D001402; https://id.nlm.nih.gov/mesh/D001172; https://id.nlm.nih.gov/mesh/D015415; https://id.nlm.nih.gov/mesh/D002454; https://id.nlm.nih.gov/mesh/D055252; https://id.nlm.nih.gov/mesh/D018920; https://id.nlm.nih.gov/mesh/D016207; https://id.nlm.nih.gov/mesh/D018836; https://id.nlm.nih.gov/mesh/D008213; https://id.nlm.nih.gov/mesh/D008264; https://id.nlm.nih.gov/mesh/D010586; https://id.nlm.nih.gov/mesh/D013601
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/