In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids
ABSTRACT: Dietary linoleic (LA) and alpha-linolenic (LN) acids are extensively isomerized and hydrogenated by rumen microbes, and this activity can further contribute to the fatty acid profile of ruminant-derived food products. Objective: To evaluate the effects of LA:LN ratio in lipid supplements o...
- Autores:
-
Castillo Vargas, Julián Andrés
Olivera Ángel, Martha
Mambro Ribeiro, Claudio Vaz Di
Daza Caicedo, Edgar Eduardo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/10718
- Acceso en línea:
- http://hdl.handle.net/10495/10718
- Palabra clave:
- Fatty acids
Kinetics of biohydrogenation
Lipid supplement
Multi-compartmental model
Hermodynamics of biohydrogenation
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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| dc.title.spa.fl_str_mv |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| dc.title.translated.spa.fl_str_mv |
Cinética de biohidrogenación ruminal in vitro de los ácidos linoleico y alfa-linolénico mezclados |
| title |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| spellingShingle |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids Fatty acids Kinetics of biohydrogenation Lipid supplement Multi-compartmental model Hermodynamics of biohydrogenation |
| title_short |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| title_full |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| title_fullStr |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| title_full_unstemmed |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| title_sort |
In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acids |
| dc.creator.fl_str_mv |
Castillo Vargas, Julián Andrés Olivera Ángel, Martha Mambro Ribeiro, Claudio Vaz Di Daza Caicedo, Edgar Eduardo |
| dc.contributor.author.none.fl_str_mv |
Castillo Vargas, Julián Andrés Olivera Ángel, Martha Mambro Ribeiro, Claudio Vaz Di Daza Caicedo, Edgar Eduardo |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biogénesis |
| dc.subject.none.fl_str_mv |
Fatty acids Kinetics of biohydrogenation Lipid supplement Multi-compartmental model Hermodynamics of biohydrogenation |
| topic |
Fatty acids Kinetics of biohydrogenation Lipid supplement Multi-compartmental model Hermodynamics of biohydrogenation |
| description |
ABSTRACT: Dietary linoleic (LA) and alpha-linolenic (LN) acids are extensively isomerized and hydrogenated by rumen microbes, and this activity can further contribute to the fatty acid profile of ruminant-derived food products. Objective: To evaluate the effects of LA:LN ratio in lipid supplements on the rumen biohydrogenation kinetics of LA and LN, as well as on the trans-vaccenic acid (VA) production, using an in vitro system. Methods: Rumen fluid was collected from a fistulated steer, diluted with incubation buffer, and then incubated with 500 mg of kikuyu grass (Cenchrus clandestinus) supplemented with 16.3 mg of different LA:LN mixtures (100:0, 75:25, 50:50, 25:75 or 0:100). Incubations were performed in triplicate for a period of 0, 2, 4, 6, 8 or 16 hours. Differences between treatments were evaluated in a completely randomized design. Alternatively, computational chemistry was used to determine the changes in the Gibbs free energy (ΔGrxn) at 39 °C for the principal steps of LA and LN ruminal biohydrogenation. Results: Partial replacement of LA by LN decreased the VA concentration and its accumulation rate; it also increased the stearic acid concentration and the rates of transfer from LA to conjugated linoleic acid (CLA), and from CLA to VA. The conversion from CLAto VA(ΔGrxn = -2.65 kJ/mol) was more spontaneousthan that from trans-11, cis-15 octadecadienoic acid (TA) to VA (ΔGrxn = -0.29 kJ/mol). Conclusion: The LA:LN ratio in lipids can modulate LA and LN biohydrogenation (BH) kinetics, as well as the VA production in the rumen. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2019-03-06T21:17:34Z |
| dc.date.available.none.fl_str_mv |
2019-03-06T21:17:34Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Vargas J, Olivera M, Ribeiro C, Daza E. In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acid. Rev Colomb Cienc Pecu. 2018; 31(1):213-222. |
| dc.identifier.issn.none.fl_str_mv |
0120-0690 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/10718 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.rccp.v31n3a06 |
| dc.identifier.eissn.none.fl_str_mv |
2256-2958 |
| identifier_str_mv |
Vargas J, Olivera M, Ribeiro C, Daza E. In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acid. Rev Colomb Cienc Pecu. 2018; 31(1):213-222. 0120-0690 10.17533/udea.rccp.v31n3a06 2256-2958 |
| url |
http://hdl.handle.net/10495/10718 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
| dc.relation.citationendpage.spa.fl_str_mv |
222 |
| dc.relation.citationissue.spa.fl_str_mv |
3 |
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213 |
| dc.relation.citationvolume.spa.fl_str_mv |
31 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Colombiana de Ciencias Pecuarias |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidad de Antioquia, Facultad de Ciencias Agrarias |
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Medellín, Colombia |
| institution |
Universidad de Antioquia |
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Castillo Vargas, Julián AndrésOlivera Ángel, MarthaMambro Ribeiro, Claudio Vaz DiDaza Caicedo, Edgar EduardoBiogénesis2019-03-06T21:17:34Z2019-03-06T21:17:34Z2017Vargas J, Olivera M, Ribeiro C, Daza E. In vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acid. Rev Colomb Cienc Pecu. 2018; 31(1):213-222.0120-0690http://hdl.handle.net/10495/1071810.17533/udea.rccp.v31n3a062256-2958ABSTRACT: Dietary linoleic (LA) and alpha-linolenic (LN) acids are extensively isomerized and hydrogenated by rumen microbes, and this activity can further contribute to the fatty acid profile of ruminant-derived food products. Objective: To evaluate the effects of LA:LN ratio in lipid supplements on the rumen biohydrogenation kinetics of LA and LN, as well as on the trans-vaccenic acid (VA) production, using an in vitro system. Methods: Rumen fluid was collected from a fistulated steer, diluted with incubation buffer, and then incubated with 500 mg of kikuyu grass (Cenchrus clandestinus) supplemented with 16.3 mg of different LA:LN mixtures (100:0, 75:25, 50:50, 25:75 or 0:100). Incubations were performed in triplicate for a period of 0, 2, 4, 6, 8 or 16 hours. Differences between treatments were evaluated in a completely randomized design. Alternatively, computational chemistry was used to determine the changes in the Gibbs free energy (ΔGrxn) at 39 °C for the principal steps of LA and LN ruminal biohydrogenation. Results: Partial replacement of LA by LN decreased the VA concentration and its accumulation rate; it also increased the stearic acid concentration and the rates of transfer from LA to conjugated linoleic acid (CLA), and from CLA to VA. The conversion from CLAto VA(ΔGrxn = -2.65 kJ/mol) was more spontaneousthan that from trans-11, cis-15 octadecadienoic acid (TA) to VA (ΔGrxn = -0.29 kJ/mol). Conclusion: The LA:LN ratio in lipids can modulate LA and LN biohydrogenation (BH) kinetics, as well as the VA production in the rumen.RESUMEN: los acidos linoleico (LA) y alfa-linolénico (LN) de la dieta son extensivamente isomerizados y biohidrogenados por los microorganismos ruminales, lo cual puede contribuir al perfil de ácidos grasos de los productos derivados de rumiantes. Objetivo: evaluar el efecto de la relación LA:LN en suplementos lipídicos sobre la cinética de biohidrogenación ruminal del LA y LN, como también sobre la producción del ácido trans-vaccenico (VA), usando un sistema in vitro. Métodos: se colectó fluido ruminal de un toro fistulado, el cual fue diluido con buffer de incubación y posteriormente incubado con 500 mg de pasto kikuyo (Cenchrus clandestinus) suplementado con 16,3 mg de diferentes mezclas de LA:LN (100:0, 75:25, 50:50, 25:75, o 0:100). Las incubaciones fueron desarrolladas en triplicado durante 0, 2, 4, 6, 8 o 16 horas. Diferencias entre tratamientos fueron evaluadas mediante un modelo completamente al azar. Alternativamente, se determinaron los cambios en energía libre de Gibbs (ΔGrxn) a 39 °C para los pasos principales de la biohidrogenación del LA y LN, usando química computacional. Resultados: la sustitución parcial de LA por LN disminuyó la concentración de VA y su tasa de acumulación, como también incrementó la concentración de ácido esteárico y las tasas de transferencia de LA para ácido linoleico conjugado (CLA) y de CLA para VA. La conversión de CLA para VA (ΔGrxn = -2,65 kJ/mol) fue mas espontanea que la conversión del ácido trans-11, cis-15 octadecadienoico (TA) para VA (ΔGrxn = -0,29 kJ/mol). Conclusiones: la relación LA:LN en lípidos puede modular la cinética de biohidrogenación (BH) del LA y LN y la producción de VA en el rumen.COL0066561application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/4.0/https://creativecommons.org/licenses/by-nc-sa/2.5/co/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Fatty acidsKinetics of biohydrogenationLipid supplementMulti-compartmental modelHermodynamics of biohydrogenationIn vitro rumen biohydrogenation kinetics of mixed linoleic and alfa-linolenic acidsCinética de biohidrogenación ruminal in vitro de los ácidos linoleico y alfa-linolénico mezcladosArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRev. Colomb. Cienc. 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