Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production
ABSTRACT: Polyhydroxyalkanoates (PHA) are fully biodegradable biopolymers of microbial origin, with physicochemical characteristics similar to those of conventional synthetic plastics. However, their current production is highly expensive mainly due to the substrate or culture medium employed. In th...
- Autores:
-
Acosta Cárdenas, Alejandro
Alcaraz Zapata, Wilman
Cardona Betancur, Mariana
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2018
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/21563
- Acceso en línea:
- http://hdl.handle.net/10495/21563
- Palabra clave:
- Biopolímeros
Biopolymers
Polihidroxialcanoatos
Polyhydroxyalkanoates
Cupriavidus necator
Melaza
Molasses
Vinaza
Vinasse
http://aims.fao.org/aos/agrovoc/c_4889
http://aims.fao.org/aos/agrovoc/c_27624
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
| id |
UDEA2_540f51a00e56cd07712325d9436278f9 |
|---|---|
| oai_identifier_str |
oai:bibliotecadigital.udea.edu.co:10495/21563 |
| network_acronym_str |
UDEA2 |
| network_name_str |
Repositorio UdeA |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| dc.title.translated.spa.fl_str_mv |
Melazas y vinaza de la caña de azúcar como sustrato para la producción de polihidroxialcanoatos (PHA) |
| title |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| spellingShingle |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production Biopolímeros Biopolymers Polihidroxialcanoatos Polyhydroxyalkanoates Cupriavidus necator Melaza Molasses Vinaza Vinasse http://aims.fao.org/aos/agrovoc/c_4889 http://aims.fao.org/aos/agrovoc/c_27624 |
| title_short |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| title_full |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| title_fullStr |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| title_full_unstemmed |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| title_sort |
Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) production |
| dc.creator.fl_str_mv |
Acosta Cárdenas, Alejandro Alcaraz Zapata, Wilman Cardona Betancur, Mariana |
| dc.contributor.author.none.fl_str_mv |
Acosta Cárdenas, Alejandro Alcaraz Zapata, Wilman Cardona Betancur, Mariana |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Biotransformación |
| dc.subject.decs.none.fl_str_mv |
Biopolímeros Biopolymers Polihidroxialcanoatos Polyhydroxyalkanoates Cupriavidus necator |
| topic |
Biopolímeros Biopolymers Polihidroxialcanoatos Polyhydroxyalkanoates Cupriavidus necator Melaza Molasses Vinaza Vinasse http://aims.fao.org/aos/agrovoc/c_4889 http://aims.fao.org/aos/agrovoc/c_27624 |
| dc.subject.agrovoc.none.fl_str_mv |
Melaza Molasses Vinaza Vinasse |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_4889 http://aims.fao.org/aos/agrovoc/c_27624 |
| description |
ABSTRACT: Polyhydroxyalkanoates (PHA) are fully biodegradable biopolymers of microbial origin, with physicochemical characteristics similar to those of conventional synthetic plastics. However, their current production is highly expensive mainly due to the substrate or culture medium employed. In this research, a mixture of cane molasses and residual vinasse from the alcohol industry was evaluated as a substrate using the reference strain Ralstonia eutrophaATCC 17699 to produce PHAs. The results showed the potential of the mixture as a culture medium, after reaching polymer concentrations of 3.17 g/L in MSM medium and 2.71 g/L in M/V medium with a molasses/vinasse ratio of 25/75. There was a sugar (sucrose,fructose, and glucose) consumption yield of 70.7%, and a biopolymer accumulation of 97.8% with respect to the biomass produced. The biopolymer characterization showed a correlation with the standard polyhydroxybutyrate sample - PHB of 99.25%. |
| publishDate |
2018 |
| dc.date.issued.none.fl_str_mv |
2018 |
| dc.date.accessioned.none.fl_str_mv |
2021-08-06T15:31:29Z |
| dc.date.available.none.fl_str_mv |
2021-08-06T15:31:29Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| status_str |
publishedVersion |
| dc.identifier.issn.none.fl_str_mv |
0012-7353 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/21563 |
| dc.identifier.doi.none.fl_str_mv |
10.15446/dyna.v85n206.68279 |
| dc.identifier.eissn.none.fl_str_mv |
2346-2183 |
| identifier_str_mv |
0012-7353 10.15446/dyna.v85n206.68279 2346-2183 |
| url |
http://hdl.handle.net/10495/21563 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Dyna |
| dc.relation.citationendpage.spa.fl_str_mv |
225 |
| dc.relation.citationissue.spa.fl_str_mv |
206 |
| dc.relation.citationstartpage.spa.fl_str_mv |
220 |
| dc.relation.citationvolume.spa.fl_str_mv |
85 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Dyna |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ https://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.extent.spa.fl_str_mv |
6 |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Minas, Centro de Publicaciones |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
| bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstreams/931e5e32-5568-45c2-b2d8-3e56cfefeb45/download https://bibliotecadigital.udea.edu.co/bitstreams/7c808a23-3b63-4586-91a0-095a7e2fa954/download https://bibliotecadigital.udea.edu.co/bitstreams/18105159-b576-46db-b66f-afdbf59de0fb/download https://bibliotecadigital.udea.edu.co/bitstreams/cc524361-4ab0-4b65-ad09-89631f926ef5/download https://bibliotecadigital.udea.edu.co/bitstreams/eb8fc4b1-bf6c-40ae-9f8d-2bb2857e87fb/download |
| bitstream.checksum.fl_str_mv |
2676d389624fc43dd8b6d425562d0c0a b88b088d9957e670ce3b3fbe2eedbc13 8a4605be74aa9ea9d79846c1fba20a33 4545c5b1356753e07e6179b5a7bd4aa0 ae43897dac1bea789b28c370d8615601 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional de la Universidad de Antioquia |
| repository.mail.fl_str_mv |
aplicacionbibliotecadigitalbiblioteca@udea.edu.co |
| _version_ |
1851052594948997120 |
| spelling |
Acosta Cárdenas, AlejandroAlcaraz Zapata, WilmanCardona Betancur, MarianaGrupo de Biotransformación2021-08-06T15:31:29Z2021-08-06T15:31:29Z20180012-7353http://hdl.handle.net/10495/2156310.15446/dyna.v85n206.682792346-2183ABSTRACT: Polyhydroxyalkanoates (PHA) are fully biodegradable biopolymers of microbial origin, with physicochemical characteristics similar to those of conventional synthetic plastics. However, their current production is highly expensive mainly due to the substrate or culture medium employed. In this research, a mixture of cane molasses and residual vinasse from the alcohol industry was evaluated as a substrate using the reference strain Ralstonia eutrophaATCC 17699 to produce PHAs. The results showed the potential of the mixture as a culture medium, after reaching polymer concentrations of 3.17 g/L in MSM medium and 2.71 g/L in M/V medium with a molasses/vinasse ratio of 25/75. There was a sugar (sucrose,fructose, and glucose) consumption yield of 70.7%, and a biopolymer accumulation of 97.8% with respect to the biomass produced. The biopolymer characterization showed a correlation with the standard polyhydroxybutyrate sample - PHB of 99.25%.RESUMEN: Los Polihidroxialcanoatos (PHAs) son biopolímeros de origen microbiano totalmente biodegradables con características físicoquímicas similares a los plásticos sintéticos convencionales, sin embargo, su producción actual es altamente costosa debido principalmente al sustrato o medio de cultivo. En esta investigación se evaluó como sustrato, una mezcla de melaza de caña y vinaza residuales de la industria licorera empleando la cepa referencia Ralstonia eutrophaATCC 17699 para la producción de PHAs. Los resultados obtenidos mostraron potencial en el uso de este medio de cultivo, alcanzando concentraciones de polímero de 3,17 g/L en medio MMS y de 2,71 g/L en el medio M/V con una relación melaza/vinaza de 25/75. El rendimiento de consumo de azucares (sacarosa, fructosa y glucosa) fue del 70,7% y una acumulación del biopolímero del 97,8% respecto a la biomasa producida. La caracterización del biopolímero presento una correlación con la muestra patrón de polihidroxibutirato - PHB del 99,25%.COL00669916application/pdfengUniversidad Nacional de Colombia, Facultad de Minas, Centro de PublicacionesMedellín, Colombiahttp://creativecommons.org/licenses/by-nc-nd/2.5/co/https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Sugarcane molasses and vinasse as a substrate for polyhydroxyalkanoates (PHA) productionMelazas y vinaza de la caña de azúcar como sustrato para la producción de polihidroxialcanoatos (PHA)Artículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBiopolímerosBiopolymersPolihidroxialcanoatosPolyhydroxyalkanoatesCupriavidus necatorMelazaMolassesVinazaVinassehttp://aims.fao.org/aos/agrovoc/c_4889http://aims.fao.org/aos/agrovoc/c_27624Dyna22520622085DynaPublicationORIGINALAcostaAlejandro_2018_SugarcaneVinassePHA.pdfAcostaAlejandro_2018_SugarcaneVinassePHA.pdfArtículo de investigaciónapplication/pdf321677https://bibliotecadigital.udea.edu.co/bitstreams/931e5e32-5568-45c2-b2d8-3e56cfefeb45/download2676d389624fc43dd8b6d425562d0c0aMD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstreams/7c808a23-3b63-4586-91a0-095a7e2fa954/downloadb88b088d9957e670ce3b3fbe2eedbc13MD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/18105159-b576-46db-b66f-afdbf59de0fb/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTAcostaAlejandro_2018_SugarcaneVinassePHA.pdf.txtAcostaAlejandro_2018_SugarcaneVinassePHA.pdf.txtExtracted texttext/plain29888https://bibliotecadigital.udea.edu.co/bitstreams/cc524361-4ab0-4b65-ad09-89631f926ef5/download4545c5b1356753e07e6179b5a7bd4aa0MD54falseAnonymousREADTHUMBNAILAcostaAlejandro_2018_SugarcaneVinassePHA.pdf.jpgAcostaAlejandro_2018_SugarcaneVinassePHA.pdf.jpgGenerated Thumbnailimage/jpeg16356https://bibliotecadigital.udea.edu.co/bitstreams/eb8fc4b1-bf6c-40ae-9f8d-2bb2857e87fb/downloadae43897dac1bea789b28c370d8615601MD55falseAnonymousREAD10495/21563oai:bibliotecadigital.udea.edu.co:10495/215632025-03-27 00:47:59.81http://creativecommons.org/licenses/by-nc-nd/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |