Human spleen contains different subsets of dendritic cells and regulatory T lymphocytes

ABSTRACT: Most knowledge about dendritic cells (DCs) and regulatory T cells in humans has been gathered from circulating cells but little is known about their frequency and distribution in lymphoid organs. This report shows the frequency, phenotype and location of DCs and regulatory T cells in deceased organ donors' spleens. As determined by flow cytometry, conventional/myeloid DCs (cDCs) CD11c(high)HLA-DR(+)CD123(-/low) were 2.3 +/- 0.9% and LIN(-) HLA-DR(+)CD11c(high) 2.1 +/- 0.3% of total spleen cells. Mature CD11c(high)HLA-DR(+)CD83(+) were 1.5 +/- 0.8% and 1.0 +/- 1.6% immature CD11c(high)HLA-DR(+)CD83(-) cDC. There were 0.3 +/- 0.3% plasmacytoid DCs (pDC) CD11c(-/low)HLA-DR(+)CD123(high) and 0.3 +/- 0.1% LIN(-)HLA DR(+)CD123(high). Cells expressing cDCs markers, BDCA-1 and BDCA-3, and pDCs markers BDCA-2 and BDCA-4 were observed in higher frequencies than DCs with other phenotypes evaluated. CD11c(+), CD123(+) and CD83(+) cells were located in subcapsular zone, T cells areas and B-cell follicles. CD4(+)CD25(high) Tregs were 0.2 +/- 0.2% and CD8(+)CD28(-) comprised 11.5 +/- 8.1% of spleen lymphocytes. FOXP3(+) cells were found in T- and B-cell areas. The improvement in cell separation, manipulation and expansion techniques, will facilitate the manipulation of donor spleen cells as a part of protocols for induction and maintenance of allograft tolerance or treatment of autoimmune diseases.

• Autores: Velásquez Lopera, Margarita María; García Moreno, Luis Fernando; Correa Londoño, Luis Alfonso
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2008
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/41220
• Acceso en línea: https://hdl.handle.net/10495/41220
• Palabra clave: Biomarcadores; Biomarkers; Células Dendríticas; Dendritic Cells; Citometría de Flujo; Flow Cytometry; Factores de Transcripción Forkhead; Forkhead Transcription Factors; Inmunohistoquímica; Immunohistochemistry; Subunidad alfa del Receptor de Interleucina-2; Interleukin-2 Receptor alpha Subunit; Bazo; Spleen; Subgrupos de Linfocitos T; T-Lymphocyte Subsets; https://id.nlm.nih.gov/mesh/D015415; https://id.nlm.nih.gov/mesh/D003713; https://id.nlm.nih.gov/mesh/D005434; https://id.nlm.nih.gov/mesh/D051858; https://id.nlm.nih.gov/mesh/D007150; https://id.nlm.nih.gov/mesh/D053645; https://id.nlm.nih.gov/mesh/D013154; https://id.nlm.nih.gov/mesh/D016176
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-nd/4.0/