Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia
ABSTRACT: The presence of genotoxic stress in eight ichthyic species in two tropical lentic environments of Colombia [cachimbero (Santander) and Ayapel (Cordoba) floodplains lakes] was evaluated by the micronuclei test. After capturing and identifying each specimen, morphometric measurements were ta...
- Autores:
-
Palacio Betancur, Isabel Cristina
Palacio Baena, Jaime Alberto
Camargo Guerrero, Mauricio
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2009
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/20929
- Acceso en línea:
- http://hdl.handle.net/10495/20929
https://revistas.udea.edu.co/index.php/actbio/article/view/4730
- Palabra clave:
- Genotoxicity
Genotoxicidad
Micronúcleos
Nucleolus
Ambiente lentico
Lentic environment
Especies ícticas
http://aims.fao.org/aos/agrovoc/c_12139
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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| dc.title.spa.fl_str_mv |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| dc.title.translated.spa.fl_str_mv |
Aplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de Colombia |
| title |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| spellingShingle |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia Genotoxicity Genotoxicidad Micronúcleos Nucleolus Ambiente lentico Lentic environment Especies ícticas http://aims.fao.org/aos/agrovoc/c_12139 |
| title_short |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| title_full |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| title_fullStr |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| title_full_unstemmed |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| title_sort |
Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in Colombia |
| dc.creator.fl_str_mv |
Palacio Betancur, Isabel Cristina Palacio Baena, Jaime Alberto Camargo Guerrero, Mauricio |
| dc.contributor.author.none.fl_str_mv |
Palacio Betancur, Isabel Cristina Palacio Baena, Jaime Alberto Camargo Guerrero, Mauricio |
| dc.contributor.researchgroup.spa.fl_str_mv |
Genética Regeneración y Cáncer Grupo de Investigación en Gestión y Modelación Ambiental (GAIA) |
| dc.subject.decs.none.fl_str_mv |
Genotoxicity Genotoxicidad |
| topic |
Genotoxicity Genotoxicidad Micronúcleos Nucleolus Ambiente lentico Lentic environment Especies ícticas http://aims.fao.org/aos/agrovoc/c_12139 |
| dc.subject.lemb.none.fl_str_mv |
Micronúcleos Nucleolus |
| dc.subject.agrovoc.none.fl_str_mv |
Ambiente lentico Lentic environment |
| dc.subject.proposal.spa.fl_str_mv |
Especies ícticas |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_12139 |
| description |
ABSTRACT: The presence of genotoxic stress in eight ichthyic species in two tropical lentic environments of Colombia [cachimbero (Santander) and Ayapel (Cordoba) floodplains lakes] was evaluated by the micronuclei test. After capturing and identifying each specimen, morphometric measurements were taken, and a blood sample drawn through cardiac or caudal vein puncture. Blood smears were stained with Giemsa and the frequency of micronuclei was quantified in 2000 cells per individual. A total of 11 species, 128 individuals, and 256.000 erythrocytes were evaluated. the condition factor for each captured specimen was calculated and a K-W analysis was applied in order to compare the number of MN among the different species in each lentic systems. The results showed a statistically significant relation between the condition factor and the frequency of micronucleated erythrocytes for the Ayapel floodplain lake. The less human-intervened floodplain lake (Cachimbero) showed the highest number of specimens with zero micronucleus, being Hoplias malabaricus the most sensitive species. The contrary occurs in the Ayapel floodplain lake contaminated with alluvial gold mining residues, where Ctenolucius sujeta and the native Cyphocharax magdalenae species presented the highest micronuclei averages. Our results further suggest that both sensitive and non-sensitive species should be included as sentinels in biomonitoring studies in Colombia. |
| publishDate |
2009 |
| dc.date.issued.none.fl_str_mv |
2009 |
| dc.date.accessioned.none.fl_str_mv |
2021-07-16T20:43:05Z |
| dc.date.available.none.fl_str_mv |
2021-07-16T20:43:05Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Palacio, I., Palacio, J., & Camargo, M. (2009). Aplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de Colombia. Actualidades Biológicas, 31(90), 67-77. https://revistas.udea.edu.co/index.php/actbio/article/view/4730 |
| dc.identifier.issn.none.fl_str_mv |
0304-3584 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/20929 |
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2145-7166 |
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https://revistas.udea.edu.co/index.php/actbio/article/view/4730 |
| identifier_str_mv |
Palacio, I., Palacio, J., & Camargo, M. (2009). Aplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de Colombia. Actualidades Biológicas, 31(90), 67-77. https://revistas.udea.edu.co/index.php/actbio/article/view/4730 0304-3584 2145-7166 |
| url |
http://hdl.handle.net/10495/20929 https://revistas.udea.edu.co/index.php/actbio/article/view/4730 |
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eng |
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eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Actual. Biol. |
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77 |
| dc.relation.citationissue.spa.fl_str_mv |
90 |
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67 |
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31 |
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Actualidades Biológicas |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Universidad de Antioquia, Facultad de Ciencias Exactas y Naturales, Instituto de Biología |
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Medellín, Colombia |
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Universidad de Antioquia |
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Palacio Betancur, Isabel CristinaPalacio Baena, Jaime AlbertoCamargo Guerrero, MauricioGenética Regeneración y CáncerGrupo de Investigación en Gestión y Modelación Ambiental (GAIA)2021-07-16T20:43:05Z2021-07-16T20:43:05Z2009Palacio, I., Palacio, J., & Camargo, M. (2009). Aplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de Colombia. Actualidades Biológicas, 31(90), 67-77. https://revistas.udea.edu.co/index.php/actbio/article/view/47300304-3584http://hdl.handle.net/10495/209292145-7166https://revistas.udea.edu.co/index.php/actbio/article/view/4730ABSTRACT: The presence of genotoxic stress in eight ichthyic species in two tropical lentic environments of Colombia [cachimbero (Santander) and Ayapel (Cordoba) floodplains lakes] was evaluated by the micronuclei test. After capturing and identifying each specimen, morphometric measurements were taken, and a blood sample drawn through cardiac or caudal vein puncture. Blood smears were stained with Giemsa and the frequency of micronuclei was quantified in 2000 cells per individual. A total of 11 species, 128 individuals, and 256.000 erythrocytes were evaluated. the condition factor for each captured specimen was calculated and a K-W analysis was applied in order to compare the number of MN among the different species in each lentic systems. The results showed a statistically significant relation between the condition factor and the frequency of micronucleated erythrocytes for the Ayapel floodplain lake. The less human-intervened floodplain lake (Cachimbero) showed the highest number of specimens with zero micronucleus, being Hoplias malabaricus the most sensitive species. The contrary occurs in the Ayapel floodplain lake contaminated with alluvial gold mining residues, where Ctenolucius sujeta and the native Cyphocharax magdalenae species presented the highest micronuclei averages. Our results further suggest that both sensitive and non-sensitive species should be included as sentinels in biomonitoring studies in Colombia.RESUMEN: Se evaluó la presencia de estrés genotóxico en algunas especies ícticas en dos ambientes lénticos tropicales de Colombia [Ciénaga de Cachimbero (Santander) y Ciénaga de Ayapel (Córdoba)] a través de la aplicación de la prueba de micronúcleos. Después de la captura de los peces y la identificación de las especies se extrajo la muestra de sangre por medio de punción cardiaca y se tomaron las medidas morfométricas. las muestras se tiñeron con Giemsa y se cuantificó la frecuencia de micronúcleos en 2.000 células por individuo. Se calculó el factor de condición para cada uno de los ejemplares capturados y se aplicó un análisis de K-W para comparar el número de MN entre las diferentes especies en cada una de las ciénagas y el número de MN de las especies comunes en los dos sistemas lagunares. Los resultados mostraron una relación estadísticamente significativa entre el factor de condición y la frecuencia de eritrocitos micronúcleados. Aunque no existen diferencias estadísticamente significativas entre las frecuencias de los micronúcleos de las diferentes especies en las ciénagas de cachimbero y de Ayapel, Hoplias malabaricus presentó un promedio de micronucleos muy superior a las demás especies en la ciénaga de Cachim-bero y Ctenolucius sujeta y Cyphocharax magdalena para la ciénaga de Ayapel. Nuestros resultados sugieren que tanto especies sensitivas como no sensitivas podrían ser incluidas como especies centinelas en los estudios de biomonitoreo en Colombia.COL0006769COL000983211application/pdfengUniversidad de Antioquia, Facultad de Ciencias Exactas y Naturales, Instituto de BiologíaMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/4.0/http://creativecommons.org/licenses/by-nc-sa/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Micronuclei test application to wild tropical ichthyic species common in two lentic environments of the low zones in ColombiaAplicación de la prueba de micronúcleos a especies ícticas silvestres frecuentes en dos ambientes lenticos de zonas bajas de ColombiaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionGenotoxicityGenotoxicidadMicronúcleosNucleolusAmbiente lenticoLentic environmentEspecies ícticashttp://aims.fao.org/aos/agrovoc/c_12139Actual. Biol.77906731Actualidades BiológicasPublicationORIGINALPalacioIsabel_2009_MicronucleiTestIchthyic.pdfPalacioIsabel_2009_MicronucleiTestIchthyic.pdfArtículo de investigaciónapplication/pdf313969https://bibliotecadigital.udea.edu.co/bitstreams/b1b46668-1569-4d39-87dc-06291474d859/downloaddf7e4199ec0d02d27e021a344928e3b3MD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstreams/29dd5cc1-b190-4787-ba4e-19bbd8e7c30d/downloade2060682c9c70d4d30c83c51448f4eedMD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/0ab3aee2-e026-4d27-a174-e42dd81696b4/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTPalacioIsabel_2009_MicronucleiTestIchthyic.pdf.txtPalacioIsabel_2009_MicronucleiTestIchthyic.pdf.txtExtracted texttext/plain38103https://bibliotecadigital.udea.edu.co/bitstreams/ff32aa42-4098-4ae8-902a-38535a53dd35/downloadb931bc83d1b525e5dbf50fe74307fa98MD54falseAnonymousREADTHUMBNAILPalacioIsabel_2009_MicronucleiTestIchthyic.pdf.jpgPalacioIsabel_2009_MicronucleiTestIchthyic.pdf.jpgGenerated Thumbnailimage/jpeg15316https://bibliotecadigital.udea.edu.co/bitstreams/b1582d4c-aa49-4d73-a2da-4f0bd69fde7e/download0b765b5253710776ee68c62c3f659adeMD55falseAnonymousREAD10495/20929oai:bibliotecadigital.udea.edu.co:10495/209292025-03-27 00:27:32.073https://creativecommons.org/licenses/by-nc-sa/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |