Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
ABSTRACT: Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years.In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, S...
- Autores:
-
Sánchez Jiménez, Miryan Margot
Isaza Agudelo, Juan Pablo
Alzate Restrepo, Juan Fernando
Olivera Ángel, Martha
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/11037
- Acceso en línea:
- http://hdl.handle.net/10495/11037
- Palabra clave:
- Genome
Sequencing
Brucella canis
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-nd/4.0/
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Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| title |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| spellingShingle |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions Genome Sequencing Brucella canis |
| title_short |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| title_full |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| title_fullStr |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| title_full_unstemmed |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| title_sort |
Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions |
| dc.creator.fl_str_mv |
Sánchez Jiménez, Miryan Margot Isaza Agudelo, Juan Pablo Alzate Restrepo, Juan Fernando Olivera Ángel, Martha |
| dc.contributor.author.none.fl_str_mv |
Sánchez Jiménez, Miryan Margot Isaza Agudelo, Juan Pablo Alzate Restrepo, Juan Fernando Olivera Ángel, Martha |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biogénesis Grupo de Parasitología, Universidad de Antioquia |
| dc.subject.none.fl_str_mv |
Genome Sequencing Brucella canis |
| topic |
Genome Sequencing Brucella canis |
| description |
ABSTRACT: Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years.In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing—CNSG in Medellin, Colombia. The assembly procedure was performed using Newbler v2.6. In the genome annotation process, each contig was analyzed independently using as reference Brucella suis ATCC 1330 chromosomes. This new genome could be useful for the development of diagnostic tools and for vaccines search as well, in order to reduce the health impact of this infection in both, dogs and humans. The sequence was deposited in EMBL-EBI with accession numbers HG803175 and HG803176 for chromosomes 1 and 2, respectively. |
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2015 |
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2015 |
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2019-05-09T15:47:30Z |
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2019-05-09T15:47:30Z |
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Artículo de investigación |
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Sánchez-Jiménez MM, Isaza JP, Alzate JF, Olivera-Angel M. Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions. Genomics. 2015 Jul;106(1):43-51. doi: 10.1016/j.ygeno.2015.03.004. |
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0888-7543 |
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http://hdl.handle.net/10495/11037 |
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10.1016/j.ygeno.2015.03.004. |
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1089-8646 |
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Sánchez-Jiménez MM, Isaza JP, Alzate JF, Olivera-Angel M. Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions. Genomics. 2015 Jul;106(1):43-51. doi: 10.1016/j.ygeno.2015.03.004. 0888-7543 10.1016/j.ygeno.2015.03.004. 1089-8646 |
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eng |
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eng |
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Genomics |
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106 |
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Genomics |
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Sánchez Jiménez, Miryan MargotIsaza Agudelo, Juan PabloAlzate Restrepo, Juan FernandoOlivera Ángel, MarthaBiogénesisGrupo de Parasitología, Universidad de Antioquia2019-05-09T15:47:30Z2019-05-09T15:47:30Z2015Sánchez-Jiménez MM, Isaza JP, Alzate JF, Olivera-Angel M. Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions. Genomics. 2015 Jul;106(1):43-51. doi: 10.1016/j.ygeno.2015.03.004.0888-7543http://hdl.handle.net/10495/1103710.1016/j.ygeno.2015.03.004.1089-8646ABSTRACT: Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years.In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing—CNSG in Medellin, Colombia. The assembly procedure was performed using Newbler v2.6. In the genome annotation process, each contig was analyzed independently using as reference Brucella suis ATCC 1330 chromosomes. This new genome could be useful for the development of diagnostic tools and for vaccines search as well, in order to reduce the health impact of this infection in both, dogs and humans. 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