Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions

ABSTRACT: Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years.In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, S...

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Autores:
Sánchez Jiménez, Miryan Margot
Isaza Agudelo, Juan Pablo
Alzate Restrepo, Juan Fernando
Olivera Ángel, Martha
Tipo de recurso:
Article of investigation
Fecha de publicación:
2015
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/11037
Acceso en línea:
http://hdl.handle.net/10495/11037
Palabra clave:
Genome
Sequencing
Brucella canis
Rights
openAccess
License
https://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.title.spa.fl_str_mv Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
title Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
spellingShingle Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
Genome
Sequencing
Brucella canis
title_short Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
title_full Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
title_fullStr Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
title_full_unstemmed Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
title_sort Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions
dc.creator.fl_str_mv Sánchez Jiménez, Miryan Margot
Isaza Agudelo, Juan Pablo
Alzate Restrepo, Juan Fernando
Olivera Ángel, Martha
dc.contributor.author.none.fl_str_mv Sánchez Jiménez, Miryan Margot
Isaza Agudelo, Juan Pablo
Alzate Restrepo, Juan Fernando
Olivera Ángel, Martha
dc.contributor.researchgroup.spa.fl_str_mv Biogénesis
Grupo de Parasitología, Universidad de Antioquia
dc.subject.none.fl_str_mv Genome
Sequencing
Brucella canis
topic Genome
Sequencing
Brucella canis
description ABSTRACT: Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years.In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing—CNSG in Medellin, Colombia. The assembly procedure was performed using Newbler v2.6. In the genome annotation process, each contig was analyzed independently using as reference Brucella suis ATCC 1330 chromosomes. This new genome could be useful for the development of diagnostic tools and for vaccines search as well, in order to reduce the health impact of this infection in both, dogs and humans. The sequence was deposited in EMBL-EBI with accession numbers HG803175 and HG803176 for chromosomes 1 and 2, respectively.
publishDate 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2019-05-09T15:47:30Z
dc.date.available.none.fl_str_mv 2019-05-09T15:47:30Z
dc.type.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Sánchez-Jiménez MM, Isaza JP, Alzate JF, Olivera-Angel M. Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions. Genomics. 2015 Jul;106(1):43-51. doi: 10.1016/j.ygeno.2015.03.004.
dc.identifier.issn.none.fl_str_mv 0888-7543
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dc.identifier.doi.none.fl_str_mv 10.1016/j.ygeno.2015.03.004.
dc.identifier.eissn.none.fl_str_mv 1089-8646
identifier_str_mv Sánchez-Jiménez MM, Isaza JP, Alzate JF, Olivera-Angel M. Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions. Genomics. 2015 Jul;106(1):43-51. doi: 10.1016/j.ygeno.2015.03.004.
0888-7543
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dc.language.iso.spa.fl_str_mv eng
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dc.relation.ispartofjournalabbrev.spa.fl_str_mv Genomics
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dc.relation.citationvolume.spa.fl_str_mv 106
dc.relation.ispartofjournal.spa.fl_str_mv Genomics
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spelling Sánchez Jiménez, Miryan MargotIsaza Agudelo, Juan PabloAlzate Restrepo, Juan FernandoOlivera Ángel, MarthaBiogénesisGrupo de Parasitología, Universidad de Antioquia2019-05-09T15:47:30Z2019-05-09T15:47:30Z2015Sánchez-Jiménez MM, Isaza JP, Alzate JF, Olivera-Angel M. Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions. Genomics. 2015 Jul;106(1):43-51. doi: 10.1016/j.ygeno.2015.03.004.0888-7543http://hdl.handle.net/10495/1103710.1016/j.ygeno.2015.03.004.1089-8646ABSTRACT: Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years.In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing—CNSG in Medellin, Colombia. The assembly procedure was performed using Newbler v2.6. In the genome annotation process, each contig was analyzed independently using as reference Brucella suis ATCC 1330 chromosomes. This new genome could be useful for the development of diagnostic tools and for vaccines search as well, in order to reduce the health impact of this infection in both, dogs and humans. 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