Recruitment of plasma cells from IL-21-dependent and IL-21-independent immune reactions to the bone marrow

ABSTRACT: Bone marrow plasma cells (BMPC) are the correlate of humoral immunity, consistently releasing antibodies into the bloodstream. It remains unclear if BMPC reflect different activation environments or maturation of their precursors. Here we define human BMPC heterogeneity and track the recruitment of antibody-secreting cells (ASC) from SARS-CoV-2 vaccine immune reactions to the bone marrow (BM). Trajectories based on single-cell transcriptomes and repertoires of peripheral and BM ASC reveal sequential colonisation of BMPC compartments. In activated B cells, IL-21 suppresses CD19 expression, indicating that CD19low-BMPC are derived from follicular, while CD19high-BMPC originate from extrafollicular immune reactions. In primary immune reactions, both CD19low- and CD19high-BMPC compartments are populated. In secondary immune reactions, most BMPC are recruited to CD19high-BMPC compartments, reflecting their origin from extrafollicular reactivations of memory B cells. A pattern also observable in vaccinated-convalescent individuals and upon diphtheria/tetanus/pertussis recall-vaccination. Thus, BMPC diversity reflects the evolution of a given humoral immune response.

• Autores: Rincón Arévalo, Héctor Julián; Ferreira Gomes, Marta; Chen, Yidan; Durek, Pawel; Heinrich, Frederik; Bauer, Laura; Franziska, Szelinski; Guerra, Gabriela Maria; Stefanski, Ana Luisa; Niedobitek, Antonia; Wiedemann, Annika; Bondareva, Marina
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2024
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/42168
• Acceso en línea: https://hdl.handle.net/10495/42168
• Palabra clave: Células Productoras de Anticuerpos; Antibody-Producing Cells; Antígenos CD19; Antigens, CD19; Linfocitos B; B-Lymphocytes; Células de la Médula Ósea; Bone Marrow Cells; Médula Ósea; Bone Marrow; COVID-19; Vacuna contra Difteria, Tétanos y Tos Ferina; Diphtheria-Tetanus-Pertussis Vaccine; Inmunidad Humoral; Immunity, Humoral; Interleucinas; Interleukins; Células Plasmáticas; Plasma Cells; SARS-CoV-2; Análisis de la Célula Individual; Single-Cell Analysis; Vacunación; Vaccination; https://id.nlm.nih.gov/mesh/D000921; https://id.nlm.nih.gov/mesh/D018941; https://id.nlm.nih.gov/mesh/D001402; https://id.nlm.nih.gov/mesh/D001854; https://id.nlm.nih.gov/mesh/D001853; https://id.nlm.nih.gov/mesh/D000086382; https://id.nlm.nih.gov/mesh/D015721; https://id.nlm.nih.gov/mesh/D056724; https://id.nlm.nih.gov/mesh/D007378; https://id.nlm.nih.gov/mesh/D010950; https://id.nlm.nih.gov/mesh/D000086402; https://id.nlm.nih.gov/mesh/D059010; https://id.nlm.nih.gov/mesh/D014611
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/