Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area

ABSTRACT: Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their distribution along Latin America through an immunological approach we designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA...

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Autores:
Risso, Marikena
Sartor, Paula
Burgos, Juan
Briceño, Luis
Rodríguez, Eva
Guhl, Felipe
Triana Chávez, Omar
Espinoza, Berta
Monteón Padilla, Víctor Manuel
Russomando, Graciela
Schijman, Alejandro
Bottasso, Oscar
Leguizamon, María Susana
Tipo de recurso:
Article of investigation
Fecha de publicación:
2011
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/26765
Acceso en línea:
http://hdl.handle.net/10495/26765
Palabra clave:
Enfermedad de Chagas
Chagas Disease
Enfermedades Endémicas
Endemic Diseases
Trypanosoma cruzi
América Latina
Latin America
Anticuerpos Antiprotozoarios - sangre
Antibodies, Protozoan - blood
Antígenos de Protozoos
Antigens, Protozoan
Western Blotting
Blotting, Western
Inmunofenotipificación
Immunophenotyping
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
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network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
title Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
spellingShingle Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
Enfermedad de Chagas
Chagas Disease
Enfermedades Endémicas
Endemic Diseases
Trypanosoma cruzi
América Latina
Latin America
Anticuerpos Antiprotozoarios - sangre
Antibodies, Protozoan - blood
Antígenos de Protozoos
Antigens, Protozoan
Western Blotting
Blotting, Western
Inmunofenotipificación
Immunophenotyping
title_short Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
title_full Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
title_fullStr Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
title_full_unstemmed Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
title_sort Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area
dc.creator.fl_str_mv Risso, Marikena
Sartor, Paula
Burgos, Juan
Briceño, Luis
Rodríguez, Eva
Guhl, Felipe
Triana Chávez, Omar
Espinoza, Berta
Monteón Padilla, Víctor Manuel
Russomando, Graciela
Schijman, Alejandro
Bottasso, Oscar
Leguizamon, María Susana
dc.contributor.author.none.fl_str_mv Risso, Marikena
Sartor, Paula
Burgos, Juan
Briceño, Luis
Rodríguez, Eva
Guhl, Felipe
Triana Chávez, Omar
Espinoza, Berta
Monteón Padilla, Víctor Manuel
Russomando, Graciela
Schijman, Alejandro
Bottasso, Oscar
Leguizamon, María Susana
dc.contributor.researchgroup.spa.fl_str_mv Biología y Control de Enfermedades Infecciosas
dc.subject.decs.none.fl_str_mv Enfermedad de Chagas
Chagas Disease
Enfermedades Endémicas
Endemic Diseases
Trypanosoma cruzi
América Latina
Latin America
Anticuerpos Antiprotozoarios - sangre
Antibodies, Protozoan - blood
Antígenos de Protozoos
Antigens, Protozoan
Western Blotting
Blotting, Western
Inmunofenotipificación
Immunophenotyping
topic Enfermedad de Chagas
Chagas Disease
Enfermedades Endémicas
Endemic Diseases
Trypanosoma cruzi
América Latina
Latin America
Anticuerpos Antiprotozoarios - sangre
Antibodies, Protozoan - blood
Antígenos de Protozoos
Antigens, Protozoan
Western Blotting
Blotting, Western
Inmunofenotipificación
Immunophenotyping
description ABSTRACT: Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their distribution along Latin America through an immunological approach we designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA II (TSSA-WB). These antigens are expressed by T. cruzi I (TCI; now TcI) and T. cruzi II (TCII; reclassified as TcII to TcVI) parasites. TSSA-WB showed good concordance with genotyping tests. An unexpected frequency of TSSA II recognition was observed in Colombia, Venezuela, and Mexico (northern region of Latin America). In Argentina and Paraguay (southern region), immunophenotyping confirmed the already reported TCII (TcII to TcVI) dominance. The lineage distribution between these regions showed significant difference but not among countries within them (except for Colombia and Venezuela). TSSA-WB shows TCII emergence in the northern region where TCI was reported as dominant or even as the unique T. cruzi lineage infecting humans.
publishDate 2011
dc.date.issued.none.fl_str_mv 2011
dc.date.accessioned.none.fl_str_mv 2022-03-22T17:05:42Z
dc.date.available.none.fl_str_mv 2022-03-22T17:05:42Z
dc.type.spa.fl_str_mv Artículo de investigación
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
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dc.identifier.citation.spa.fl_str_mv Risso, M., Sartor, P. A., Burgos, J., Briceño, L., Rodríguez, E., Guhl, F., Triana, O., Espinoza, B., Monteón, V., Russomando, G., Schijman, A., Bottasso, O., & Leguizamón, M. (2011). Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area. The American journal of tropical medicine and hygiene, 84(1), 78–84. https://doi.org/10.4269/ajtmh.2011.10-0177
dc.identifier.issn.none.fl_str_mv 0002-9637
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/26765
dc.identifier.doi.none.fl_str_mv 10.4269/ajtmh.2011.10-0177
dc.identifier.eissn.none.fl_str_mv 1476-1645
identifier_str_mv Risso, M., Sartor, P. A., Burgos, J., Briceño, L., Rodríguez, E., Guhl, F., Triana, O., Espinoza, B., Monteón, V., Russomando, G., Schijman, A., Bottasso, O., & Leguizamón, M. (2011). Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area. The American journal of tropical medicine and hygiene, 84(1), 78–84. https://doi.org/10.4269/ajtmh.2011.10-0177
0002-9637
10.4269/ajtmh.2011.10-0177
1476-1645
url http://hdl.handle.net/10495/26765
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Am. J. Trop. Med. Hyg.
dc.relation.citationendpage.spa.fl_str_mv 84
dc.relation.citationissue.spa.fl_str_mv 1
dc.relation.citationstartpage.spa.fl_str_mv 78
dc.relation.citationvolume.spa.fl_str_mv 84
dc.relation.ispartofjournal.spa.fl_str_mv American Journal of Tropical Medicine and Hygiene
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dc.publisher.spa.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.publisher.place.spa.fl_str_mv Baltimore, Estados Unidos
institution Universidad de Antioquia
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spelling Risso, MarikenaSartor, PaulaBurgos, JuanBriceño, LuisRodríguez, EvaGuhl, FelipeTriana Chávez, OmarEspinoza, BertaMonteón Padilla, Víctor ManuelRussomando, GracielaSchijman, AlejandroBottasso, OscarLeguizamon, María SusanaBiología y Control de Enfermedades Infecciosas2022-03-22T17:05:42Z2022-03-22T17:05:42Z2011Risso, M., Sartor, P. A., Burgos, J., Briceño, L., Rodríguez, E., Guhl, F., Triana, O., Espinoza, B., Monteón, V., Russomando, G., Schijman, A., Bottasso, O., & Leguizamón, M. (2011). Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic area. The American journal of tropical medicine and hygiene, 84(1), 78–84. https://doi.org/10.4269/ajtmh.2011.10-01770002-9637http://hdl.handle.net/10495/2676510.4269/ajtmh.2011.10-01771476-1645ABSTRACT: Genotyping studies show a polarized geographic distribution of Trypanosoma cruzi lineages in humans. Here, we assessed their distribution along Latin America through an immunological approach we designated Western blot (WB) assay with Trypomastigote small-surface antigen (TSSA) I and TSSA II (TSSA-WB). These antigens are expressed by T. cruzi I (TCI; now TcI) and T. cruzi II (TCII; reclassified as TcII to TcVI) parasites. TSSA-WB showed good concordance with genotyping tests. An unexpected frequency of TSSA II recognition was observed in Colombia, Venezuela, and Mexico (northern region of Latin America). In Argentina and Paraguay (southern region), immunophenotyping confirmed the already reported TCII (TcII to TcVI) dominance. The lineage distribution between these regions showed significant difference but not among countries within them (except for Colombia and Venezuela). TSSA-WB shows TCII emergence in the northern region where TCI was reported as dominant or even as the unique T. cruzi lineage infecting humans.COL00078657application/pdfengAmerican Society of Tropical Medicine and HygieneBaltimore, Estados Unidoshttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Immunological identification of Trypanosoma cruzi lineages in human infection along the endemic areaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionEnfermedad de ChagasChagas DiseaseEnfermedades EndémicasEndemic DiseasesTrypanosoma cruziAmérica LatinaLatin AmericaAnticuerpos Antiprotozoarios - sangreAntibodies, Protozoan - bloodAntígenos de ProtozoosAntigens, ProtozoanWestern BlottingBlotting, WesternInmunofenotipificaciónImmunophenotypingAm. J. Trop. Med. 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