Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts
RESUMO: Veneno de cobra continua a ser um problema importante de saúde em muitos países da América Latina. Apesar dos avanços na terapia antiveneno, os efeitos locais causados por fosfolipases A2 miotóxica (PLA2) presentes no veneno, ainda persistem. Em busca de alternativas para antagonizar a ativi...
- Autores:
-
Pereañez Jiménez, Jaime Andrés
Lobo Echeverri, Tatiana
Rojano, Benjamín Alberto
Vargas Muñoz, Leidy Johana
Fernández Culma, Maritza
Gaviria, Carlos Andrés
Núñez Rangel, Vitelbina
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2010
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/37209
- Acceso en línea:
- https://hdl.handle.net/10495/37209
- Palabra clave:
- Inhibidores de Fosfolipasa A2
Phospholipase A2 Inhibitors
Venenos Elapídicos
Elapid Venoms
Extractos Vegetales
Plant Extracts
Antioxidantes
Antioxidants
Bothrops asper
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| title |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| spellingShingle |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts Inhibidores de Fosfolipasa A2 Phospholipase A2 Inhibitors Venenos Elapídicos Elapid Venoms Extractos Vegetales Plant Extracts Antioxidantes Antioxidants Bothrops asper |
| title_short |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| title_full |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| title_fullStr |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| title_full_unstemmed |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| title_sort |
Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extracts |
| dc.creator.fl_str_mv |
Pereañez Jiménez, Jaime Andrés Lobo Echeverri, Tatiana Rojano, Benjamín Alberto Vargas Muñoz, Leidy Johana Fernández Culma, Maritza Gaviria, Carlos Andrés Núñez Rangel, Vitelbina |
| dc.contributor.author.none.fl_str_mv |
Pereañez Jiménez, Jaime Andrés Lobo Echeverri, Tatiana Rojano, Benjamín Alberto Vargas Muñoz, Leidy Johana Fernández Culma, Maritza Gaviria, Carlos Andrés Núñez Rangel, Vitelbina |
| dc.contributor.researchgroup.spa.fl_str_mv |
Toxinología, Alternativas Terapéuticas y Alimentarias |
| dc.subject.decs.none.fl_str_mv |
Inhibidores de Fosfolipasa A2 Phospholipase A2 Inhibitors Venenos Elapídicos Elapid Venoms Extractos Vegetales Plant Extracts Antioxidantes Antioxidants |
| topic |
Inhibidores de Fosfolipasa A2 Phospholipase A2 Inhibitors Venenos Elapídicos Elapid Venoms Extractos Vegetales Plant Extracts Antioxidantes Antioxidants Bothrops asper |
| dc.subject.proposal.spa.fl_str_mv |
Bothrops asper |
| description |
RESUMO: Veneno de cobra continua a ser um problema importante de saúde em muitos países da América Latina. Apesar dos avanços na terapia antiveneno, os efeitos locais causados por fosfolipases A2 miotóxica (PLA2) presentes no veneno, ainda persistem. Em busca de alternativas para antagonizar a atividade da PLA2 do veneno de Bothrops asper, foram selecionados 36 extratos pertencentes a dezessete famílias de plantas vasculares e briófitas. Uma inibição significativa da atividade enzimática de PLA2 presente no veneno de B. asper foi observada em onze extratos. Além disso, a atividade antioxidante dos extratos foi avaliada. Os resultados evidenciaram uma correlação estatisticamente significativa entre os extratos com ação inibitória contra a PLA2 e aqueles com atividade antioxidante. Também, a quantidade de fenóis foi avaliada e foi encontrada uma relação entre a atividade biológica e a presença dessas substâncias. Nove extratos foram testados contra uma fração do veneno rico em PLA2 básica (Fx-V B. asper), resultando em um efeito inibitório na atividade desta fração da PLA2 na faixa de 30-80%. Esta atividade foi apoiada pela inibição que esses extratos apresentaram na citotoxicidade causada pelo Fx-V B. asper sobre mioblastos C2C12 de músculo esquelético de murino. Os resultados podem indicar a minimização dos esforços na busca de inibidores da PLA2, com foco nas amostras com propriedades antioxidantes conhecidas |
| publishDate |
2010 |
| dc.date.issued.none.fl_str_mv |
2010 |
| dc.date.accessioned.none.fl_str_mv |
2023-11-08T21:52:18Z |
| dc.date.available.none.fl_str_mv |
2023-11-08T21:52:18Z |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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0102-695X |
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https://hdl.handle.net/10495/37209 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0102-695X2010005000030 |
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1981-528X |
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0102-695X 10.1590/S0102-695X2010005000030 1981-528X |
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https://hdl.handle.net/10495/37209 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Bras. Farmacogn. |
| dc.relation.citationendpage.spa.fl_str_mv |
916 |
| dc.relation.citationissue.spa.fl_str_mv |
6 |
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910 |
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20 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Brasileira de Farmacognosia |
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Sociedade Brasileira de Farmacognosia |
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Curitiba, Brasil |
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Universidad de Antioquia |
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Pereañez Jiménez, Jaime AndrésLobo Echeverri, TatianaRojano, Benjamín AlbertoVargas Muñoz, Leidy JohanaFernández Culma, MaritzaGaviria, Carlos AndrésNúñez Rangel, VitelbinaToxinología, Alternativas Terapéuticas y Alimentarias2023-11-08T21:52:18Z2023-11-08T21:52:18Z20100102-695Xhttps://hdl.handle.net/10495/3720910.1590/S0102-695X20100050000301981-528XRESUMO: Veneno de cobra continua a ser um problema importante de saúde em muitos países da América Latina. Apesar dos avanços na terapia antiveneno, os efeitos locais causados por fosfolipases A2 miotóxica (PLA2) presentes no veneno, ainda persistem. Em busca de alternativas para antagonizar a atividade da PLA2 do veneno de Bothrops asper, foram selecionados 36 extratos pertencentes a dezessete famílias de plantas vasculares e briófitas. Uma inibição significativa da atividade enzimática de PLA2 presente no veneno de B. asper foi observada em onze extratos. Além disso, a atividade antioxidante dos extratos foi avaliada. Os resultados evidenciaram uma correlação estatisticamente significativa entre os extratos com ação inibitória contra a PLA2 e aqueles com atividade antioxidante. Também, a quantidade de fenóis foi avaliada e foi encontrada uma relação entre a atividade biológica e a presença dessas substâncias. Nove extratos foram testados contra uma fração do veneno rico em PLA2 básica (Fx-V B. asper), resultando em um efeito inibitório na atividade desta fração da PLA2 na faixa de 30-80%. Esta atividade foi apoiada pela inibição que esses extratos apresentaram na citotoxicidade causada pelo Fx-V B. asper sobre mioblastos C2C12 de músculo esquelético de murino. Os resultados podem indicar a minimização dos esforços na busca de inibidores da PLA2, com foco nas amostras com propriedades antioxidantes conhecidasABSTRACT: Snakebite continues to be a significant health problem in many countries of Latin America. Even though, there has been an improvement in the antivenom therapy, the local effects caused by myotoxic phospholipases A2 (PLA2) present in the venoms, still persist. In search for alternatives to antagonize the PLA2 activity of Bothrops asper’s venom, 36 extracts belonging to seventeen families of vascular plants and bryophytes were screened. A significant inhibition of the enzymatic activity of PLA2 present in B. asper’s whole venom was seen in eleven of these extracts. In addition, the antioxidant activity of all the extracts was evaluated. The results evidenced a significant statistical correlation between extracts with an inhibitory effect against PLA2 and those with an antioxidant activity. Moreover, the amount of phenols was quantified finding a relationship between the bioactivity and the presence of these compounds. Nine extracts were screened against a fraction of the venom rich in basic PLA2 (Fx-V B. asper), exhibiting an inhibitory effect on PLA2 activity of this fraction in a range of 30-80%. This activity was supported by the inhibition that these extracts presented on the cytotoxicity caused by Fx-V B. asper on murine skeletal muscle C2C12 myoblasts. The results obtained, could point to minimize efforts in the search of PLA2 inhibitors by focusing in samples with known antioxidant properties.COL00144767application/pdfengSociedade Brasileira de FarmacognosiaCuritiba, Brasilhttps://creativecommons.org/licenses/by-nc-sa/4.0/http://creativecommons.org/licenses/by-nc-sa/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Correlation of the inhibitory activity of phospholipase A2 snake venom and the antioxidant activity of Colombian plant extractsArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionInhibidores de Fosfolipasa A2Phospholipase A2 InhibitorsVenenos ElapídicosElapid VenomsExtractos VegetalesPlant ExtractsAntioxidantesAntioxidantsBothrops asperRev. Bras. 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