Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia
ABSTRACT: Introduction: In Colombia there are three Anopheles species implicated in malaria transmission as primary vectors; however, the local role of some Anopheles species must still be defined. Objective: To determine the abundance, composition and natural infection rates for Anopheles mosquitoe...
- Autores:
-
Montoya Martínez, Carolina
Bascuñán García, Ana Priscila
Rodríguez Zabala, Julián
Correa Ochoa, Margarita María
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/20098
- Acceso en línea:
- http://hdl.handle.net/10495/20098
- Palabra clave:
- Plasmodium
Anopheles
Malaria
Disease vectors
Vectores de enfermedades
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
http://id.nlm.nih.gov/mesh/D010961
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-nd/4.0/
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| dc.title.spa.fl_str_mv |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| dc.title.translated.spa.fl_str_mv |
Abundancia, composición e infección natural de mosquitos Anopheles en dos regiones endémicas para malaria en Colombia |
| title |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| spellingShingle |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia Plasmodium Anopheles Malaria Disease vectors Vectores de enfermedades Reacción en Cadena de la Polimerasa Polymerase Chain Reaction http://id.nlm.nih.gov/mesh/D010961 |
| title_short |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| title_full |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| title_fullStr |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| title_full_unstemmed |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| title_sort |
Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of Colombia |
| dc.creator.fl_str_mv |
Montoya Martínez, Carolina Bascuñán García, Ana Priscila Rodríguez Zabala, Julián Correa Ochoa, Margarita María |
| dc.contributor.author.none.fl_str_mv |
Montoya Martínez, Carolina Bascuñán García, Ana Priscila Rodríguez Zabala, Julián Correa Ochoa, Margarita María |
| dc.contributor.researchgroup.spa.fl_str_mv |
Microbiología Molecular |
| dc.subject.mesh.none.fl_str_mv |
Plasmodium |
| topic |
Plasmodium Anopheles Malaria Disease vectors Vectores de enfermedades Reacción en Cadena de la Polimerasa Polymerase Chain Reaction http://id.nlm.nih.gov/mesh/D010961 |
| dc.subject.decs.none.fl_str_mv |
Anopheles Malaria Disease vectors Vectores de enfermedades Reacción en Cadena de la Polimerasa Polymerase Chain Reaction |
| dc.subject.meshuri.none.fl_str_mv |
http://id.nlm.nih.gov/mesh/D010961 |
| description |
ABSTRACT: Introduction: In Colombia there are three Anopheles species implicated in malaria transmission as primary vectors; however, the local role of some Anopheles species must still be defined. Objective: To determine the abundance, composition and natural infection rates for Anopheles mosquitoes with Plasmodium spp. in two malaria-endemic regions of Colombia. Materials and methods: Anopheles mosquitoes were collected using the human-landing catches and while resting in livestock corrals in nine localities of two malaria-endemic regions of Colombia. Mosquitoes were morphologically identified and confirmed by PCR-RFLP-ITS2. Identified mosquitoes were processed and tested for Plasmodium parasite infection by ELISA and ssrRNA-based nested PCR. Results: We collected 1,963 Anopheles mosquitoes corresponding to nine species. The most abundant species were Anopheles nuneztovari (53.5%) and A. darlingi (34.5%), followed by A. triannulatus s.l. (6%), and other species (≈5.9%). Three species were naturally infected with Plasmodium spp.: A. darlingi, A. nuneztovari and A. triannulatus s.l. Conclusions: Natural infection of A. darlingi and A. nuneztovari indicate that these malaria vectors continue to be effective carriers of Plasmodium in the localities under study in Valle del Cauca and Chocó. Additionally, the infected A. triannulatus s.l. collected in livestock corrals in the locality of the department of Córdoba suggests the need for further studies to define the epidemiological importance of this species given its abundance and opportunistic anthropophilic behavior. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2021-06-11T13:18:51Z |
| dc.date.available.none.fl_str_mv |
2021-06-11T13:18:51Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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0120-4157 |
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http://hdl.handle.net/10495/20098 |
| dc.identifier.doi.none.fl_str_mv |
10.7705/biomedica.v37i0.3553 |
| dc.identifier.eissn.none.fl_str_mv |
2590-7379 |
| identifier_str_mv |
0120-4157 10.7705/biomedica.v37i0.3553 2590-7379 |
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http://hdl.handle.net/10495/20098 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Biomédica |
| dc.relation.citationendpage.spa.fl_str_mv |
105 |
| dc.relation.citationissue.spa.fl_str_mv |
Supl 2 |
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98 |
| dc.relation.citationvolume.spa.fl_str_mv |
37 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Biomédica |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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openAccess |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Instituto Nacional de Salud |
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Bogotá, Colombia |
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Universidad de Antioquia |
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Montoya Martínez, CarolinaBascuñán García, Ana PriscilaRodríguez Zabala, JuliánCorrea Ochoa, Margarita MaríaMicrobiología Molecular2021-06-11T13:18:51Z2021-06-11T13:18:51Z20170120-4157http://hdl.handle.net/10495/2009810.7705/biomedica.v37i0.35532590-7379ABSTRACT: Introduction: In Colombia there are three Anopheles species implicated in malaria transmission as primary vectors; however, the local role of some Anopheles species must still be defined. Objective: To determine the abundance, composition and natural infection rates for Anopheles mosquitoes with Plasmodium spp. in two malaria-endemic regions of Colombia. Materials and methods: Anopheles mosquitoes were collected using the human-landing catches and while resting in livestock corrals in nine localities of two malaria-endemic regions of Colombia. Mosquitoes were morphologically identified and confirmed by PCR-RFLP-ITS2. Identified mosquitoes were processed and tested for Plasmodium parasite infection by ELISA and ssrRNA-based nested PCR. Results: We collected 1,963 Anopheles mosquitoes corresponding to nine species. The most abundant species were Anopheles nuneztovari (53.5%) and A. darlingi (34.5%), followed by A. triannulatus s.l. (6%), and other species (≈5.9%). Three species were naturally infected with Plasmodium spp.: A. darlingi, A. nuneztovari and A. triannulatus s.l. Conclusions: Natural infection of A. darlingi and A. nuneztovari indicate that these malaria vectors continue to be effective carriers of Plasmodium in the localities under study in Valle del Cauca and Chocó. Additionally, the infected A. triannulatus s.l. collected in livestock corrals in the locality of the department of Córdoba suggests the need for further studies to define the epidemiological importance of this species given its abundance and opportunistic anthropophilic behavior.RESUMEN: Introducción. En Colombia hay tres especies de mosquitos Anopheles implicadas como vectores primarios en la transmisión de la malaria o paludismo; sin embargo, el rol local de algunas especies de Anopheles aún debe determinarse. Objetivo. Determinar la abundancia, la composición y la infección natural de mosquitos anofelinos con Plasmodium spp. en dos regiones endémicas de malaria en Colombia. Materiales y métodos. Se recolectaron mosquitos del género Anopheles usando los métodos de recolección con cebo humano y en reposo en corrales de ganado vacuno, en nueve localidades de dos regiones endémicas para malaria en Colombia. Los especímenes se identificaron morfológicamente y se confirmaron por reacción en cadena de la polimerasa (PCR) de los polimorfismos en la longitud de los fragmentos de restricción (Restriction Fragment Length Polymorphism, RFLP) en el espaciador intergénico ribosómico nuclear 2 (Internal Transcribed Spacer, ITS-2) (PCR-RFLP-ITS2). Los especímenes se procesaron y analizaron mediante ELISA y PCR anidada basada en la subunidad pequeña del ARN ribosómico (small subunit ribosomal RNA, ssrRNA) para determinar la infección por Plasmodium. Resultados. Se recolectaron 1.963 mosquitos Anopheles correspondientes a nueve especies. Anopheles nuneztovari fue la especie predominante (53,5 %), seguida por A. darlingi (34,5 %), A. triannulatus s.l. (6 %) y por otras especies (≈5,9 %). Tres especies se encontraron naturalmente infectadas con Plasmodium spp.: A. darlingi, A. nuneztovari y A. triannulatus s.l. Conclusiones. La infección natural de A. darlingi y A. nuneztovari indica que estos vectores primarios siguen siendo actores principales en la transmisión de malaria en las localidades estudiadas de los departamentos del Valle del Cauca y Chocó. Además, el espécimen A. triannulatus s.l. infectado, recolectado en corrales de animales de la localidad estudiada en el departamento de Córdoba, indica que existe la necesidad de estudios futuros para establecer la importancia epidemiológica de esta especie dada su abundancia y comportamiento antropofílico oportunista.COL00137468application/pdfengInstituto Nacional de SaludBogotá, Colombiahttps://creativecommons.org/licenses/by-nc-nd/4.0/http://creativecommons.org/licenses/by-nc-nd/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2PlasmodiumAnophelesMalariaDisease vectorsVectores de enfermedadesReacción en Cadena de la PolimerasaPolymerase Chain Reactionhttp://id.nlm.nih.gov/mesh/D010961Abundance, composition and natural infection of Anopheles mosquitoes from two malaria-endemic regions of ColombiaAbundancia, composición e infección natural de mosquitos Anopheles en dos regiones endémicas para malaria en ColombiaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBiomédica105Supl 29837BiomédicaPublicationORIGINALMontoyaCarolina_2017_AnophelesMosquitoesMalaria-Endemic.pdfMontoyaCarolina_2017_AnophelesMosquitoesMalaria-Endemic.pdfArtículo de investigaciónapplication/pdf268638https://bibliotecadigital.udea.edu.co/bitstreams/c09e71d8-d205-4e4a-98f9-a6f786e43605/download8dcf48b38063831440bcc028cbec86e9MD51trueAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/de09da43-d293-45c0-a2b2-7d51277585b8/download8a4605be74aa9ea9d79846c1fba20a33MD52falseAnonymousREADTEXTMontoyaCarolina_2017_AnophelesMosquitoesMalaria-Endemic.pdf.txtMontoyaCarolina_2017_AnophelesMosquitoesMalaria-Endemic.pdf.txtExtracted texttext/plain37353https://bibliotecadigital.udea.edu.co/bitstreams/06cb8b99-82d2-4fbd-ab76-767d2f97a65a/download92d8ff96a989a83e26c7730da4aacfebMD53falseAnonymousREADTHUMBNAILMontoyaCarolina_2017_AnophelesMosquitoesMalaria-Endemic.pdf.jpgMontoyaCarolina_2017_AnophelesMosquitoesMalaria-Endemic.pdf.jpgGenerated Thumbnailimage/jpeg14648https://bibliotecadigital.udea.edu.co/bitstreams/c1585ec2-c792-4b2b-95fd-04856b485912/download5d2255219b944690a900008a6f422154MD54falseAnonymousREAD10495/20098oai:bibliotecadigital.udea.edu.co:10495/200982025-03-26 21:21:06.745https://creativecommons.org/licenses/by-nc-nd/4.0/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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 |