Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia
ABSTRACT: Acute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools ar...
- Autores:
-
Calderón Ruiz, Paula Andrea
Tobón Castaño, Alberto
Vélez Tobón, Gabriel Jaime
Holguín Rocha, Andrés Felipe
Haist, Gregor
Daniel, Tim
Koliopoulos, Philip
Londoño Rentería, Berlín Luxelly
Mascus, Annina
Gehring, Stephan
Gröndah, Britta
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/41465
- Acceso en línea:
- https://hdl.handle.net/10495/41465
- Palabra clave:
- Malaria
Dengue
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Reverse Transcriptase Polymerase Chain Reaction
Colombia
ELISA
https://id.nlm.nih.gov/mesh/D020133
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D020133
https://id.nlm.nih.gov/mesh/D003715
https://id.nlm.nih.gov/mesh/D008288
https://id.nlm.nih.gov/mesh/D004797
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by/4.0/
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Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| spellingShingle |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia Malaria Dengue Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Reverse Transcriptase Polymerase Chain Reaction Colombia ELISA https://id.nlm.nih.gov/mesh/D020133 https://id.nlm.nih.gov/mesh/D003105 https://id.nlm.nih.gov/mesh/D020133 https://id.nlm.nih.gov/mesh/D003715 https://id.nlm.nih.gov/mesh/D008288 https://id.nlm.nih.gov/mesh/D004797 |
| title_short |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_full |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_fullStr |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_full_unstemmed |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| title_sort |
Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia |
| dc.creator.fl_str_mv |
Calderón Ruiz, Paula Andrea Tobón Castaño, Alberto Vélez Tobón, Gabriel Jaime Holguín Rocha, Andrés Felipe Haist, Gregor Daniel, Tim Koliopoulos, Philip Londoño Rentería, Berlín Luxelly Mascus, Annina Gehring, Stephan Gröndah, Britta |
| dc.contributor.author.none.fl_str_mv |
Calderón Ruiz, Paula Andrea Tobón Castaño, Alberto Vélez Tobón, Gabriel Jaime Holguín Rocha, Andrés Felipe Haist, Gregor Daniel, Tim Koliopoulos, Philip Londoño Rentería, Berlín Luxelly Mascus, Annina Gehring, Stephan Gröndah, Britta |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo Malaria |
| dc.subject.mesh.none.fl_str_mv |
Malaria |
| topic |
Malaria Dengue Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Reverse Transcriptase Polymerase Chain Reaction Colombia ELISA https://id.nlm.nih.gov/mesh/D020133 https://id.nlm.nih.gov/mesh/D003105 https://id.nlm.nih.gov/mesh/D020133 https://id.nlm.nih.gov/mesh/D003715 https://id.nlm.nih.gov/mesh/D008288 https://id.nlm.nih.gov/mesh/D004797 |
| dc.subject.decs.none.fl_str_mv |
Dengue Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Reverse Transcriptase Polymerase Chain Reaction Colombia ELISA |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D020133 https://id.nlm.nih.gov/mesh/D003105 https://id.nlm.nih.gov/mesh/D020133 https://id.nlm.nih.gov/mesh/D003715 https://id.nlm.nih.gov/mesh/D008288 https://id.nlm.nih.gov/mesh/D004797 |
| description |
ABSTRACT: Acute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools are required to identify the causative agents. A descriptive cross-sectional study was conducted to evaluate the circulation and differential diagnosis of six pathogens in two regions of Colombia. The results obtained via multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) were comparable to those obtained using rapid tests conducted at the time of patient enrollment. Of 155 patients evaluated, 25 (16.1%) and 16 (10.3%) were positive for malaria and dengue, respectively; no samples were positive for any of the other infectious agents tested. In most cases, m-RT-PCR-ELISA confirmed the results previously obtained through rapid testing. Keywords: Colombia; acute febrile syndrome; dengue; malaria; multiplex RT-PCR-ELISA; rapid tests. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-08-26T17:30:58Z |
| dc.date.available.none.fl_str_mv |
2024-08-26T17:30:58Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Calderon-Ruiz P, Haist G, Mascus A, Holguin-Rocha AF, Koliopoulos P, Daniel T, Velez G, Londono-Renteria B, Gröndahl B, Tobon-Castano A, Gehring S. Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia. Trop Med Infect Dis. 2023 Oct 6;8(10):466. doi: 10.3390/tropicalmed8100466. |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/41465 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/tropicalmed8100466 |
| dc.identifier.eissn.none.fl_str_mv |
2414-6366 |
| identifier_str_mv |
Calderon-Ruiz P, Haist G, Mascus A, Holguin-Rocha AF, Koliopoulos P, Daniel T, Velez G, Londono-Renteria B, Gröndahl B, Tobon-Castano A, Gehring S. Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia. Trop Med Infect Dis. 2023 Oct 6;8(10):466. doi: 10.3390/tropicalmed8100466. 10.3390/tropicalmed8100466 2414-6366 |
| url |
https://hdl.handle.net/10495/41465 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Trop. Med. Infect. Dis. |
| dc.relation.citationendpage.spa.fl_str_mv |
11 |
| dc.relation.citationissue.spa.fl_str_mv |
10 |
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1 |
| dc.relation.citationvolume.spa.fl_str_mv |
8 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Tropical Medicine and Infectious Disease |
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openAccess |
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11 páginas |
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MDPI |
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Basilea, Suiza |
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Calderón Ruiz, Paula AndreaTobón Castaño, AlbertoVélez Tobón, Gabriel JaimeHolguín Rocha, Andrés FelipeHaist, GregorDaniel, TimKoliopoulos, PhilipLondoño Rentería, Berlín LuxellyMascus, AnninaGehring, StephanGröndah, BrittaGrupo Malaria2024-08-26T17:30:58Z2024-08-26T17:30:58Z2023Calderon-Ruiz P, Haist G, Mascus A, Holguin-Rocha AF, Koliopoulos P, Daniel T, Velez G, Londono-Renteria B, Gröndahl B, Tobon-Castano A, Gehring S. Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia. Trop Med Infect Dis. 2023 Oct 6;8(10):466. doi: 10.3390/tropicalmed8100466.https://hdl.handle.net/10495/4146510.3390/tropicalmed81004662414-6366ABSTRACT: Acute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools are required to identify the causative agents. A descriptive cross-sectional study was conducted to evaluate the circulation and differential diagnosis of six pathogens in two regions of Colombia. The results obtained via multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) were comparable to those obtained using rapid tests conducted at the time of patient enrollment. Of 155 patients evaluated, 25 (16.1%) and 16 (10.3%) were positive for malaria and dengue, respectively; no samples were positive for any of the other infectious agents tested. In most cases, m-RT-PCR-ELISA confirmed the results previously obtained through rapid testing. Keywords: Colombia; acute febrile syndrome; dengue; malaria; multiplex RT-PCR-ELISA; rapid tests.European Union's Horizon 2020 research and innovation programElse Kröner-Fresenius-StiftungCOL000752411 páginasapplication/pdfengMDPIBasilea, Suizahttps://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/2.5/co/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2MalariaDengueReacción en Cadena de la Polimerasa de Transcriptasa InversaReverse Transcriptase Polymerase Chain ReactionColombiaELISAhttps://id.nlm.nih.gov/mesh/D020133https://id.nlm.nih.gov/mesh/D003105https://id.nlm.nih.gov/mesh/D020133https://id.nlm.nih.gov/mesh/D003715https://id.nlm.nih.gov/mesh/D008288https://id.nlm.nih.gov/mesh/D004797Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of ColombiaArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTrop. 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