Multiplex Reverse Transcription Polymerase Chain Reaction Combined with a Microwell Hybridization Assay Screening for Arbovirus and Parasitic Infections in Febrile Patients Living in Endemic Regions of Colombia

ABSTRACT: Acute febrile syndrome is a frequent reason for medical consultations in tropical and subtropical countries where the cause could have an infectious origin. Malaria and dengue are the primary etiologies in Colombia. As such, constant epidemiological surveillance and new diagnostic tools are required to identify the causative agents. A descriptive cross-sectional study was conducted to evaluate the circulation and differential diagnosis of six pathogens in two regions of Colombia. The results obtained via multiplex reverse transcription polymerase chain reaction combined with a microwell hybridization assay (m-RT-PCR-ELISA) were comparable to those obtained using rapid tests conducted at the time of patient enrollment. Of 155 patients evaluated, 25 (16.1%) and 16 (10.3%) were positive for malaria and dengue, respectively; no samples were positive for any of the other infectious agents tested. In most cases, m-RT-PCR-ELISA confirmed the results previously obtained through rapid testing. Keywords: Colombia; acute febrile syndrome; dengue; malaria; multiplex RT-PCR-ELISA; rapid tests.

• Autores: Calderón Ruiz, Paula Andrea; Tobón Castaño, Alberto; Vélez Tobón, Gabriel Jaime; Holguín Rocha, Andrés Felipe; Haist, Gregor; Daniel, Tim; Koliopoulos, Philip; Londoño Rentería, Berlín Luxelly; Mascus, Annina; Gehring, Stephan; Gröndah, Britta
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2023
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/41465
• Acceso en línea: https://hdl.handle.net/10495/41465
• Palabra clave: Malaria; Dengue; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa; Reverse Transcriptase Polymerase Chain Reaction; Colombia; ELISA; https://id.nlm.nih.gov/mesh/D020133; https://id.nlm.nih.gov/mesh/D003105; https://id.nlm.nih.gov/mesh/D020133; https://id.nlm.nih.gov/mesh/D003715; https://id.nlm.nih.gov/mesh/D008288; https://id.nlm.nih.gov/mesh/D004797
• Rights: openAccess
• License: https://creativecommons.org/licenses/by/4.0/