Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference

ABSTRACT: Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. For C. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of a C. hominis strain isolated directly from human stool and a new genome annotation of the C. parvum Iowa reference genome.

• Autores: Isaza Agudelo, Juan Pablo; Galván Díaz, Ana Luz; Alzate Restrepo, Juan Fernando; Polanco, Víctor; Huang, Bernice; Matveyev, Andrey V.; Serrano, Myrna G.; Manque, Patricio; Buck, Gregory A.
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2015
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/42140
• Acceso en línea: https://hdl.handle.net/10495/42140
• Palabra clave: Biología Computacional; Computational Biology; Criptosporidiosis; Cryptosporidiosis; Cryptosporidium; Cryptosporidium parvum; Bases de Datos Genéticas; Databases, Genetic; Ontología de Genes; Gene Ontology; Genoma de Protozoos; Genome, Protozoan; Secuenciación de Nucleótidos de Alto Rendimiento; High-Throughput Nucleotide Sequencing; Anotación de Secuencia Molecular; Molecular Sequence Annotation; Tipificación Molecular; Molecular Typing; Filogenia; Phylogeny; https://id.nlm.nih.gov/mesh/D019295; https://id.nlm.nih.gov/mesh/D003457; https://id.nlm.nih.gov/mesh/D003458; https://id.nlm.nih.gov/mesh/D016785; https://id.nlm.nih.gov/mesh/D030541; https://id.nlm.nih.gov/mesh/D063990; https://id.nlm.nih.gov/mesh/D018503; https://id.nlm.nih.gov/mesh/D059014; https://id.nlm.nih.gov/mesh/D058977; https://id.nlm.nih.gov/mesh/D058889; https://id.nlm.nih.gov/mesh/D010802
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/