Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11

ABSTRACT: The kinetoplastid membrane protein 11 (KMP-11) has been recently described in Leishmania (Leishmania) donovani as a major component of the promastigote membrane. Two oligonucleotide primers were synthesized to PCR-amplify the entire coding region of New World Leishmania species. The Leishmania (Viannia) panamensis amplification product was cloned, sequenced and the putative amino acid sequence determined. A remarkably high degree of sequence homology was observed with the corresponding molecule of L. (L) donovani and L. (L) infantum (97% and 96%, respectively). Southern blot analysis showed that the KMP-11 locus is conformed by three copies of the gene. The L. (V) panamensis ORF was subsequently cloned in a high expression vector and the recombinant protein was induced and purified from Escherichia coli cultures. Immunoblot analysis showed that 80%, 77% and 100% sera from cutaneous, mucocutaneous and visceral leishmaniasis patients, respectively, recognized the recombinant KMP-11 protein. In a similar assay, 86% of asymptomatic Leishmania-infected individuals showed IgG antibodies against the rKMP-11. We propose that KMP-11 could be used as a serologic marker for infection and disease caused by Leishmania in America.

• Autores: Ramírez Pineda, José Robinson; Berberich, Christof Ernest; Jaramillo Ramírez, Andrés; Alonso, Carlos; Vélez Bernal, Iván Darío
• Tipo de recurso: Article of investigation
• Fecha de publicación: 1998
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39013
• Acceso en línea: https://hdl.handle.net/10495/39013
• Palabra clave: Anticuerpos Monoclonales; Antibodies, Monoclonal; Expresión Génica; Gene Expression; Leishmania guyanensis; Leishmaniasis; Proteínas de la Membrana - química; Membrane Proteins - chemistry; Proteínas Recombinantes - aislamiento y purificación; Recombinant Proteins - isolation and purification; Análisis de Secuencia de ADN; Sequence Analysis, DNA; https://id.nlm.nih.gov/mesh/D000911; https://id.nlm.nih.gov/mesh/D015870; https://id.nlm.nih.gov/mesh/D018334; https://id.nlm.nih.gov/mesh/D007896; https://id.nlm.nih.gov/mesh/D008565; https://id.nlm.nih.gov/mesh/D011994; https://id.nlm.nih.gov/mesh/D017422
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/