Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children
ABSTRACT: Blastocystis sp., is an intestinal protist with a broad host range and a high prevalence in human populations worldwide, even in developed Western countries. The publication of conflicting evidence has divided the scientific community about the pathogenic role of this parasite. Even though...
- Autores:
-
García Montoya, Gisela María
Galván Díaz, Ana Luz
Alzate Restrepo, Juan Fernando
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/43461
- Acceso en línea:
- https://hdl.handle.net/10495/43461
- Palabra clave:
- Infecciones por Blastocystis
Blastocystis Infections
Blastocystis - genética
Blastocystis - genetics
Colombia - epidemiología
Colombia - epidemiology
Niño
Child
Coinfección
Coinfection
ADN Protozoario
DNA, Protozoan
Heces - parasitología
Feces / parasitology
Variación Genética
Genetic Variation
Filogenia
Phylogeny
https://id.nlm.nih.gov/mesh/D016776
https://id.nlm.nih.gov/mesh/D016844
https://id.nlm.nih.gov/mesh/D003105
https://id.nlm.nih.gov/mesh/D002648
https://id.nlm.nih.gov/mesh/D060085
https://id.nlm.nih.gov/mesh/D016054
https://id.nlm.nih.gov/mesh/D005243
https://id.nlm.nih.gov/mesh/D014644
https://id.nlm.nih.gov/mesh/D010802
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| title |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| spellingShingle |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children Infecciones por Blastocystis Blastocystis Infections Blastocystis - genética Blastocystis - genetics Colombia - epidemiología Colombia - epidemiology Niño Child Coinfección Coinfection ADN Protozoario DNA, Protozoan Heces - parasitología Feces / parasitology Variación Genética Genetic Variation Filogenia Phylogeny https://id.nlm.nih.gov/mesh/D016776 https://id.nlm.nih.gov/mesh/D016844 https://id.nlm.nih.gov/mesh/D003105 https://id.nlm.nih.gov/mesh/D002648 https://id.nlm.nih.gov/mesh/D060085 https://id.nlm.nih.gov/mesh/D016054 https://id.nlm.nih.gov/mesh/D005243 https://id.nlm.nih.gov/mesh/D014644 https://id.nlm.nih.gov/mesh/D010802 |
| title_short |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| title_full |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| title_fullStr |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| title_full_unstemmed |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| title_sort |
Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children |
| dc.creator.fl_str_mv |
García Montoya, Gisela María Galván Díaz, Ana Luz Alzate Restrepo, Juan Fernando |
| dc.contributor.author.none.fl_str_mv |
García Montoya, Gisela María Galván Díaz, Ana Luz Alzate Restrepo, Juan Fernando |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación Clínica en Enfermedades del Niño y del Adolescente - Pediaciencias Microbiología Ambiental |
| dc.subject.decs.none.fl_str_mv |
Infecciones por Blastocystis Blastocystis Infections Blastocystis - genética Blastocystis - genetics Colombia - epidemiología Colombia - epidemiology Niño Child Coinfección Coinfection ADN Protozoario DNA, Protozoan Heces - parasitología Feces / parasitology Variación Genética Genetic Variation Filogenia Phylogeny |
| topic |
Infecciones por Blastocystis Blastocystis Infections Blastocystis - genética Blastocystis - genetics Colombia - epidemiología Colombia - epidemiology Niño Child Coinfección Coinfection ADN Protozoario DNA, Protozoan Heces - parasitología Feces / parasitology Variación Genética Genetic Variation Filogenia Phylogeny https://id.nlm.nih.gov/mesh/D016776 https://id.nlm.nih.gov/mesh/D016844 https://id.nlm.nih.gov/mesh/D003105 https://id.nlm.nih.gov/mesh/D002648 https://id.nlm.nih.gov/mesh/D060085 https://id.nlm.nih.gov/mesh/D016054 https://id.nlm.nih.gov/mesh/D005243 https://id.nlm.nih.gov/mesh/D014644 https://id.nlm.nih.gov/mesh/D010802 |
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https://id.nlm.nih.gov/mesh/D016776 https://id.nlm.nih.gov/mesh/D016844 https://id.nlm.nih.gov/mesh/D003105 https://id.nlm.nih.gov/mesh/D002648 https://id.nlm.nih.gov/mesh/D060085 https://id.nlm.nih.gov/mesh/D016054 https://id.nlm.nih.gov/mesh/D005243 https://id.nlm.nih.gov/mesh/D014644 https://id.nlm.nih.gov/mesh/D010802 |
| description |
ABSTRACT: Blastocystis sp., is an intestinal protist with a broad host range and a high prevalence in human populations worldwide, even in developed Western countries. The publication of conflicting evidence has divided the scientific community about the pathogenic role of this parasite. Even though, genetic studies on Blastocystis sp. revealed associations between genotypes and different pathogenic profiles. Conventionally, the detection of this parasite is based on microscopic or PCR methods, which offer meager or null performance in detecting mixed infections. In this work, we applied a metataxonomic NGS approach targeting the V4 region of the eukaryotic SSU-rRNA gene and classical phylogenetic methods. This approach allowed us to detect Blastocystis sp. in stool samples from infected children living in an urban setting in the city of Medellin attending the same daycare center. Phylogenetic analysis identified the subtypes present in the children as ST1, ST2, and ST3. Besides, mixed infections of subtypes ST1 + ST3 were spotted in 16% of the analyzed stool samples. |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-11-13T20:44:35Z |
| dc.date.available.none.fl_str_mv |
2024-11-13T20:44:35Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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García-Montoya GM, Galván-Díaz AL, Alzate JF. Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children. Infect Genet Evol. 2023 Sep;113:105478. doi: 10.1016/j.meegid.2023.105478. |
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1567-1348 |
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https://hdl.handle.net/10495/43461 |
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10.1016/j.meegid.2023.105478 |
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1567-7257 |
| identifier_str_mv |
García-Montoya GM, Galván-Díaz AL, Alzate JF. Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children. Infect Genet Evol. 2023 Sep;113:105478. doi: 10.1016/j.meegid.2023.105478. 1567-1348 10.1016/j.meegid.2023.105478 1567-7257 |
| url |
https://hdl.handle.net/10495/43461 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Infect. Gent. Evol. |
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9 |
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1 |
| dc.relation.citationvolume.spa.fl_str_mv |
113 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Infection, Genetics and Evolution |
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http://creativecommons.org/licenses/by/2.5/co/ |
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9 páginas |
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application/pdf |
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Elsevier |
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Ámsterdam, Países Bajos |
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García Montoya, Gisela MaríaGalván Díaz, Ana LuzAlzate Restrepo, Juan FernandoGrupo de Investigación Clínica en Enfermedades del Niño y del Adolescente - PediacienciasMicrobiología Ambiental2024-11-13T20:44:35Z2024-11-13T20:44:35Z2023García-Montoya GM, Galván-Díaz AL, Alzate JF. Metataxomics reveals Blastocystis subtypes mixed infections in Colombian children. Infect Genet Evol. 2023 Sep;113:105478. doi: 10.1016/j.meegid.2023.105478.1567-1348https://hdl.handle.net/10495/4346110.1016/j.meegid.2023.1054781567-7257ABSTRACT: Blastocystis sp., is an intestinal protist with a broad host range and a high prevalence in human populations worldwide, even in developed Western countries. The publication of conflicting evidence has divided the scientific community about the pathogenic role of this parasite. Even though, genetic studies on Blastocystis sp. revealed associations between genotypes and different pathogenic profiles. Conventionally, the detection of this parasite is based on microscopic or PCR methods, which offer meager or null performance in detecting mixed infections. In this work, we applied a metataxonomic NGS approach targeting the V4 region of the eukaryotic SSU-rRNA gene and classical phylogenetic methods. This approach allowed us to detect Blastocystis sp. in stool samples from infected children living in an urban setting in the city of Medellin attending the same daycare center. Phylogenetic analysis identified the subtypes present in the children as ST1, ST2, and ST3. Besides, mixed infections of subtypes ST1 + ST3 were spotted in 16% of the analyzed stool samples.Universidad de Antioquia. Vicerrectoría de investigación. Comité para el Desarrollo de la Investigación - CODIUniversidad de Antioquia, Escuela de MicrobiologíaCOL0040189COL00587849 páginasapplication/pdfengElsevierÁmsterdam, Países Bajoshttp://creativecommons.org/licenses/by/2.5/co/https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Metataxomics reveals Blastocystis subtypes mixed infections in Colombian childrenArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionInfecciones por BlastocystisBlastocystis InfectionsBlastocystis - genéticaBlastocystis - geneticsColombia - epidemiologíaColombia - epidemiologyNiñoChildCoinfecciónCoinfectionADN ProtozoarioDNA, ProtozoanHeces - parasitologíaFeces / parasitologyVariación GenéticaGenetic VariationFilogeniaPhylogenyhttps://id.nlm.nih.gov/mesh/D016776https://id.nlm.nih.gov/mesh/D016844https://id.nlm.nih.gov/mesh/D003105https://id.nlm.nih.gov/mesh/D002648https://id.nlm.nih.gov/mesh/D060085https://id.nlm.nih.gov/mesh/D016054https://id.nlm.nih.gov/mesh/D005243https://id.nlm.nih.gov/mesh/D014644https://id.nlm.nih.gov/mesh/D010802Infect. 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