Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis
ABSTRACT: We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cr...
- Autores:
-
Ortiz Reyes, Blanca Lucía
McEwen Ochoa, Juan Guillermo
Cepero Garcia, Ana María
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 1996
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/39541
- Acceso en línea:
- https://hdl.handle.net/10495/39541
- Palabra clave:
- Antígenos Fúngicos
Antigens, Fungal
Proteínas Fúngicas
Fungal Proteins
Peso Molecular
Molecular Weight
Paracoccidioides
Paracoccidioidomicosis
Proteínas Recombinantes
Recombinant Proteins
Paracoccidioidomycosis
https://id.nlm.nih.gov/mesh/D000946
https://id.nlm.nih.gov/mesh/D005656
https://id.nlm.nih.gov/mesh/D008970
https://id.nlm.nih.gov/mesh/D010228
https://id.nlm.nih.gov/mesh/D011994
https://id.nlm.nih.gov/mesh/D010229
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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| dc.title.spa.fl_str_mv |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| title |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| spellingShingle |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis Antígenos Fúngicos Antigens, Fungal Proteínas Fúngicas Fungal Proteins Peso Molecular Molecular Weight Paracoccidioides Paracoccidioidomicosis Proteínas Recombinantes Recombinant Proteins Paracoccidioidomycosis https://id.nlm.nih.gov/mesh/D000946 https://id.nlm.nih.gov/mesh/D005656 https://id.nlm.nih.gov/mesh/D008970 https://id.nlm.nih.gov/mesh/D010228 https://id.nlm.nih.gov/mesh/D011994 https://id.nlm.nih.gov/mesh/D010229 |
| title_short |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| title_full |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| title_fullStr |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| title_full_unstemmed |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| title_sort |
Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensis |
| dc.creator.fl_str_mv |
Ortiz Reyes, Blanca Lucía McEwen Ochoa, Juan Guillermo Cepero Garcia, Ana María |
| dc.contributor.author.none.fl_str_mv |
Ortiz Reyes, Blanca Lucía McEwen Ochoa, Juan Guillermo Cepero Garcia, Ana María |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biología Celular y Molecular CIB U. de A. U. del Rosario |
| dc.subject.decs.none.fl_str_mv |
Antígenos Fúngicos Antigens, Fungal Proteínas Fúngicas Fungal Proteins Peso Molecular Molecular Weight Paracoccidioides Paracoccidioidomicosis Proteínas Recombinantes Recombinant Proteins Paracoccidioidomycosis |
| topic |
Antígenos Fúngicos Antigens, Fungal Proteínas Fúngicas Fungal Proteins Peso Molecular Molecular Weight Paracoccidioides Paracoccidioidomicosis Proteínas Recombinantes Recombinant Proteins Paracoccidioidomycosis https://id.nlm.nih.gov/mesh/D000946 https://id.nlm.nih.gov/mesh/D005656 https://id.nlm.nih.gov/mesh/D008970 https://id.nlm.nih.gov/mesh/D010228 https://id.nlm.nih.gov/mesh/D011994 https://id.nlm.nih.gov/mesh/D010229 |
| dc.subject.meshuri.none.fl_str_mv |
https://id.nlm.nih.gov/mesh/D000946 https://id.nlm.nih.gov/mesh/D005656 https://id.nlm.nih.gov/mesh/D008970 https://id.nlm.nih.gov/mesh/D010228 https://id.nlm.nih.gov/mesh/D011994 https://id.nlm.nih.gov/mesh/D010229 |
| description |
ABSTRACT: We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis. |
| publishDate |
1996 |
| dc.date.issued.none.fl_str_mv |
1996 |
| dc.date.accessioned.none.fl_str_mv |
2024-06-02T08:11:24Z |
| dc.date.available.none.fl_str_mv |
2024-06-02T08:11:24Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Ortiz BL, Garcia AM, Restrepo A, McEwen JG. 1996. Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis. Clin Diagn Lab Immunol 3:. https://doi.org/10.1128/cdli.3.2.239-241.1996 |
| dc.identifier.issn.none.fl_str_mv |
1071-412X |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/39541 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/cdli.3.2.239-241.1996 |
| dc.identifier.eissn.none.fl_str_mv |
1098-6588 |
| identifier_str_mv |
Ortiz BL, Garcia AM, Restrepo A, McEwen JG. 1996. Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis. Clin Diagn Lab Immunol 3:. https://doi.org/10.1128/cdli.3.2.239-241.1996 1071-412X 10.1128/cdli.3.2.239-241.1996 1098-6588 |
| url |
https://hdl.handle.net/10495/39541 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Clin. Diagn. Lab. Immunol. |
| dc.relation.citationendpage.spa.fl_str_mv |
241 |
| dc.relation.citationissue.spa.fl_str_mv |
6 |
| dc.relation.citationstartpage.spa.fl_str_mv |
239 |
| dc.relation.citationvolume.spa.fl_str_mv |
5 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Clinical and Diagnostic Laboratory Immunology |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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openAccess |
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3 páginas |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
American Society for Microbiology |
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Universidad de Antioquia |
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Ortiz Reyes, Blanca LucíaMcEwen Ochoa, Juan GuillermoCepero Garcia, Ana MaríaBiología Celular y Molecular CIB U. de A. U. del Rosario2024-06-02T08:11:24Z2024-06-02T08:11:24Z1996Ortiz BL, Garcia AM, Restrepo A, McEwen JG. 1996. Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis. Clin Diagn Lab Immunol 3:. https://doi.org/10.1128/cdli.3.2.239-241.19961071-412Xhttps://hdl.handle.net/10495/3954110.1128/cdli.3.2.239-241.19961098-6588ABSTRACT: We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis.Colombia. Ministerio de Ciencia, Tecnología e Innovación - MincienciasCOL00009623 páginasapplication/pdfengAmerican Society for MicrobiologyWashington, Estados Unidoshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Immunological Characterization of a Recombinant 27-Kilodaltons Antigenic Protein from Paracoccidioides brasiliensisArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAntígenos FúngicosAntigens, FungalProteínas FúngicasFungal ProteinsPeso MolecularMolecular WeightParacoccidioidesParacoccidioidomicosisProteínas RecombinantesRecombinant ProteinsParacoccidioidomycosishttps://id.nlm.nih.gov/mesh/D000946https://id.nlm.nih.gov/mesh/D005656https://id.nlm.nih.gov/mesh/D008970https://id.nlm.nih.gov/mesh/D010228https://id.nlm.nih.gov/mesh/D011994https://id.nlm.nih.gov/mesh/D010229Clin. 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