In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis
ABSTRACT : Polyhydroxyalkanoates (PHA) are synthesised by bacteria as carbon storage material. The protein PhaG directs carbon from non-related carbon sources such as glycerol, metabolised through fatty acid de novo synthesis (FAS) pathway, with PHA synthesis. The gene that codifies for this protein...
- Autores:
-
Uribe Acosta, Melissa
Villa Restrepo, Andrés Felipe
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2019
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/29469
- Acceso en línea:
- http://hdl.handle.net/10495/29469
- Palabra clave:
- Polihidroxialcanoatos
Polyhydroxyalkanoates
Cupriavidus necator
Ralstonia eutropha H16
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| dc.title.translated.spa.fl_str_mv |
Análisis in silico de una proteína similar a phag en ralstonia eutropha h16 potencialmente involucrada en la síntesis de polihidroxialcanoatos |
| title |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| spellingShingle |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis Polihidroxialcanoatos Polyhydroxyalkanoates Cupriavidus necator Ralstonia eutropha H16 |
| title_short |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| title_full |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| title_fullStr |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| title_full_unstemmed |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| title_sort |
In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesis |
| dc.creator.fl_str_mv |
Uribe Acosta, Melissa Villa Restrepo, Andrés Felipe |
| dc.contributor.author.none.fl_str_mv |
Uribe Acosta, Melissa Villa Restrepo, Andrés Felipe |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Biotransformación |
| dc.subject.decs.none.fl_str_mv |
Polihidroxialcanoatos Polyhydroxyalkanoates Cupriavidus necator |
| topic |
Polihidroxialcanoatos Polyhydroxyalkanoates Cupriavidus necator Ralstonia eutropha H16 |
| dc.subject.proposal.spa.fl_str_mv |
Ralstonia eutropha H16 |
| description |
ABSTRACT : Polyhydroxyalkanoates (PHA) are synthesised by bacteria as carbon storage material. The protein PhaG directs carbon from non-related carbon sources such as glycerol, metabolised through fatty acid de novo synthesis (FAS) pathway, with PHA synthesis. The gene that codifies for this protein has not yet been found in the genome of Ralstonia eutropha H16, a model organism. By bioinformatic comparison to already known PhaG proteins, a PhaG-like protein was found codified by gene H16_A0147 and presence of the gene was preliminary confirmed by PCR. This is the first study that shows the presence and characteristics of a PhaGlike protein in R. eutropha H16 and represents the first step for the identification of a connection between FAS and PHA pathways in this model bacterium. Further gene deletion and enzymatic activity studies are necessary to confirm this potential relationship, which could improve industrial PHA production and utilisation of agroindustrial residues such as glycerol. |
| publishDate |
2019 |
| dc.date.issued.none.fl_str_mv |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2022-06-29T19:04:08Z |
| dc.date.available.none.fl_str_mv |
2022-06-29T19:04:08Z |
| dc.type.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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1900-2351 |
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http://hdl.handle.net/10495/29469 |
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10.33571/rpolitec.v15n29a5 |
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2256-5353 |
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1900-2351 10.33571/rpolitec.v15n29a5 2256-5353 |
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http://hdl.handle.net/10495/29469 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Politec. |
| dc.relation.citationendpage.spa.fl_str_mv |
64 |
| dc.relation.citationissue.spa.fl_str_mv |
29 |
| dc.relation.citationstartpage.spa.fl_str_mv |
55 |
| dc.relation.citationvolume.spa.fl_str_mv |
15 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Revista Politécnica |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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10 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Politécnico Colombiano Jaime Isaza Cadavid |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
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Universidad de Antioquia |
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Uribe Acosta, MelissaVilla Restrepo, Andrés FelipeGrupo de Biotransformación2022-06-29T19:04:08Z2022-06-29T19:04:08Z20191900-2351http://hdl.handle.net/10495/2946910.33571/rpolitec.v15n29a52256-5353ABSTRACT : Polyhydroxyalkanoates (PHA) are synthesised by bacteria as carbon storage material. The protein PhaG directs carbon from non-related carbon sources such as glycerol, metabolised through fatty acid de novo synthesis (FAS) pathway, with PHA synthesis. The gene that codifies for this protein has not yet been found in the genome of Ralstonia eutropha H16, a model organism. By bioinformatic comparison to already known PhaG proteins, a PhaG-like protein was found codified by gene H16_A0147 and presence of the gene was preliminary confirmed by PCR. This is the first study that shows the presence and characteristics of a PhaGlike protein in R. eutropha H16 and represents the first step for the identification of a connection between FAS and PHA pathways in this model bacterium. Further gene deletion and enzymatic activity studies are necessary to confirm this potential relationship, which could improve industrial PHA production and utilisation of agroindustrial residues such as glycerol.RESUMEN : Los polihidroxialcanoatos (PHA) son sintetizados por las bacterias como material de reserva de carbono. La proteína PhaG dirige el carbono proveniente de fuentes de carbono no relacionadas como el glicerol, que son metabolizados a través de la síntesis de ácidos grasos de novo (FAS), hacia la síntesis de PHA. El gen que codifica esta proteína no ha sido aún encontrado en el genoma de Ralstonia eutropha H16, un organismo modelo. A través de la comparación con proteínas PhaG ya conocidas, una proteína similar a PhaG, fue encontrada siendo codificada por el gen H16_A0147 y la presencia del gen confirmada preliminarmente utilizando PCR. Este es el primer estudio que muestra la presencia y características de una proteína similar a PhaG en R. eutropha H16 y representa el primer paso en la identificación de una conexión entre las rutas metabólicas FAS y de PHA en esta bacteria modelo. Estudios de bloqueo de genes y actividad enzimática son necesarios para confirmar esta relación potencial que podría mejorar la producción industrial de PHA y la utilización de residuos agroindustriales como el glicerol.COL006699110application/pdfengPolitécnico Colombiano Jaime Isaza CadavidMedellín, Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2In silico analysis of phag-like protein in ralstonia eutropha h16, potentially involved in polyhydroxyalkanoates synthesisAnálisis in silico de una proteína similar a phag en ralstonia eutropha h16 potencialmente involucrada en la síntesis de polihidroxialcanoatosArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPolihidroxialcanoatosPolyhydroxyalkanoatesCupriavidus necatorRalstonia eutropha H16Rev. 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