TNF microsatellites polymorphism is associated with rheumatoid arthritis. Confirming evidence in northwestern Colombians

ABSTRACT: Objective: To examine the contribution of tumor necrosis factor alpha (TNF) microsatellite (a to e) polymorphism to the genetic risk of developing rheumatoid arthritis (RA) in a northwestern Colombian population. Methods: This was an association study in which 108 RA patients and 222 matched individuals were enrolled. HLA-DRB1 and DQB1 polymorphisms were evaluated to examine for linkage disequilibrium between these loci and TNF micro- satellites. Genotyping was performed using denaturing polyacrylamide gels and polymerase chain reaction-sequence techniques. Results: By unconditional logistic regression analysis, the TNFa6 allele (OR=2.37, 95%CI 1.07-5.24) and the TNFb4 allele (OR=3.01, 95%CI 1.07-9.00) were observed to be associated with disease. These associations were independent of HLA-DR and HLA-DQ since linkage disequilibrium between HLA class II and TNF microsatellites was not observed. In addition, patients with the TNFa8 allele had a five times greater risk of developing extra-articular manifestations as compared to patients without this allele (OR=5.07, 95%CI 1.14-22.52), regardless of age and the duration of disease. Haplotype analysis disclosed a protective effect for TNFa7/b7/c1/d4/e3/-308G/-238G. Conclusion: These results confirm that the TNF locus exerts a primary influence on both susceptibility to and the severity of RA.

• Autores: Gómez Osorio, Luis Miguel; Ruiz Narváez, Edward A.; Pineda Tamayo, Ricardo; Rojas Villarraga, Adriana; Anaya Cabrera, Juan Manuel
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2007
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/39576
• Acceso en línea: https://hdl.handle.net/10495/39576
• Palabra clave: Artritis Reumatoide; Arthritis, Rheumatoid; Predisposición Genética a la Enfermedad; Genetic Predisposition to Disease; Desequilibrio de Ligamiento; Linkage Disequilibrium; Repeticiones de Microsatélite; Microsatellite Repeats; Polimorfismo Genético; Polymorphism, Genetic; Análisis de Regresión; Regression Analysis; Factores de Riesgo; Risk Factors; Índice de Severidad de la Enfermedad; Severity of Illness Index; Factor de Necrosis Tumoral alfa; Tumor Necrosis Factor-alpha; https://id.nlm.nih.gov/mesh/D001172; https://id.nlm.nih.gov/mesh/D020022; https://id.nlm.nih.gov/mesh/D015810; https://id.nlm.nih.gov/mesh/D018895; https://id.nlm.nih.gov/mesh/D011110; https://id.nlm.nih.gov/mesh/D012044; https://id.nlm.nih.gov/mesh/D012307; https://id.nlm.nih.gov/mesh/D012720; https://id.nlm.nih.gov/mesh/D014409
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-nd/4.0/