Mycobacterial Membrane Vesicles Administered Systemically in Mice Induce a Protective Immune Response to Surface Compartments of Mycobacterium tuberculosis

ABSTRACT:Pathogenic and nonpathogenic species of bacteria and fungi release membrane vesicles (MV), containing proteins, polysaccharides, and lipids, into the extracellular milieu. Previously, we demonstrated that several mycobacterial species, including bacillus Calmette-Guerin (BCG) and Mycobacterium tuberculosis, release MV containing lipids and proteins that subvert host immune response in a Toll-like receptor 2 (TLR2)-dependent manner (R. Prados-Rosales et al., J. Clin. Invest. 121:1471-1483, 2011, doi:10.1172/JCI44261). In this work, we analyzed the vaccine potential of MV in a mouse model and compared the effects of immunization with MV to those of standard BCG vaccination. Immunization with MV from BCG or M. tuberculosis elicited a mixed humoral and cellular response directed to both membrane and cell wall components, such as lipoproteins. However, only vaccination with M. tuberculosis MV was able to protect as well as live BCG immunization. M. tuberculosis MV boosted BCG vaccine efficacy. In summary, MV are highly immunogenic without adjuvants and elicit immune responses comparable to those achieved with BCG in protection against M. tuberculosis.

• Autores: Baena García, Andrés; Achkar, Jacqueline M.; Batista González, Ana; Carreño, Leandro J.; Casadevall, Arturo; Chan, John; Jacobs Jr, William R.; LaBaer, Joshua; Magee, D Mitchell; Porcelli, Steven A.; Prados Rosales, Rafael; Venkataswamy, Manjunatha M.; Wallstrom, Garrick; Xu, Jiayong; Yu, Xiaobo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2014
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/44190
• Acceso en línea: https://hdl.handle.net/10495/44190
• Palabra clave: Adjuvants, Immunologic; Adjuvants, Immunologic; Anticuerpos Antibacterianos; Antibodies, Bacterial; Vacuna BCG; BCG Vaccine; Ensayo de Inmunoadsorción Enzimática; Enzyme-Linked Immunosorbent Assay; Inmunidad Humoral; Immunity, Humoral; Inmunidad Innata; Immunity, Innate; Lipoproteínas; Lipoproteins; Proteínas de la Membrana; Membrane Proteins; Ratones Endogámicos C57BL; Mice, Inbred C57BL; Mycobacterium bovis; Mycobacterium tuberculosis; Tuberculosis; Vacunas contra la Tuberculosis; Tuberculosis Vaccines; https://id.nlm.nih.gov/mesh/D000276; https://id.nlm.nih.gov/mesh/D000907; https://id.nlm.nih.gov/mesh/D001500; https://id.nlm.nih.gov/mesh/D004797; https://id.nlm.nih.gov/mesh/D056724; https://id.nlm.nih.gov/mesh/D007113; https://id.nlm.nih.gov/mesh/D008074; https://id.nlm.nih.gov/mesh/D008565; https://id.nlm.nih.gov/mesh/D008810; https://id.nlm.nih.gov/mesh/D009163; https://id.nlm.nih.gov/mesh/D009169; https://id.nlm.nih.gov/mesh/D014376; https://id.nlm.nih.gov/mesh/D032581
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/