Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster
ABSTRACT: Leishmaniasis are endemic in >98 countries with 14 million people infected. Leishmania survives in mammal hosts even after treatment but the capacity to persist in healthy skin and infect sandflies from these sites is poorly understood. To demonstrate migration of Leishmania (V.) paname...
- Autores:
-
Carrillo Bonilla, Lina María
Montoya, Andrés
Arbeláez, Natalia
Cadena, Horacio
Ramírez, Jaime
Robledo Restrepo, Sara María
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/11005
- Acceso en línea:
- http://hdl.handle.net/10495/11005
- Palabra clave:
- Migración parasitaria
Parasite migration
Xenodiagnostico
Xenodiagnosis
Hsp70 PCR
Real-time DNA polymerase
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc/2.5/co/
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Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| dc.title.translated.spa.fl_str_mv |
Migración de Leishmania panamensis y su persistencia en piel sana de Hamster |
| title |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| spellingShingle |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster Migración parasitaria Parasite migration Xenodiagnostico Xenodiagnosis Hsp70 PCR Real-time DNA polymerase |
| title_short |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| title_full |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| title_fullStr |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| title_full_unstemmed |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| title_sort |
Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster |
| dc.creator.fl_str_mv |
Carrillo Bonilla, Lina María Montoya, Andrés Arbeláez, Natalia Cadena, Horacio Ramírez, Jaime Robledo Restrepo, Sara María |
| dc.contributor.author.none.fl_str_mv |
Carrillo Bonilla, Lina María Montoya, Andrés Arbeláez, Natalia Cadena, Horacio Ramírez, Jaime Robledo Restrepo, Sara María |
| dc.subject.none.fl_str_mv |
Migración parasitaria Parasite migration Xenodiagnostico Xenodiagnosis Hsp70 PCR Real-time DNA polymerase |
| topic |
Migración parasitaria Parasite migration Xenodiagnostico Xenodiagnosis Hsp70 PCR Real-time DNA polymerase |
| description |
ABSTRACT: Leishmaniasis are endemic in >98 countries with 14 million people infected. Leishmania survives in mammal hosts even after treatment but the capacity to persist in healthy skin and infect sandflies from these sites is poorly understood. To demonstrate migration of Leishmania (V.) panamensis into healty skin, persistence in these sites during chronic stages of infection and infectivity of persistant parasites to Lutzomyia longipalpis 10 hamsters were inoculated intradermally in the hind footpad, with 2x107 L. (V.) panamensis promastigotes. Hamsters were sacrificed at 13 (acute phase) and 53 (chronic phase) weeks post-infection. Biopsies from lesion, healty skin and othert tissues were screened by Hsp70 polymerase chain reaction (PCR) and DNA polymerase qPCR. Transmission of parasites to sandflies was determined by xenodiagnosis. Positivity was observed in all samples of acute infection group except the contralateral ear and chronic infection group, although the percentage of positivity in chronic infection was lower than that for acute infection. Two animals of the acute infection group and one from the chronic infection group were infective to sand flies after feeding over the lesion site. No infections were found in sandflies fed from healthy skin. Dissemination and persistence of L. (V.) panamensis was reported for first time and persisntace is confirmed for a period of 53 weeks. However, parasite load in tissues different from the inoculation site was lower and transmission to the sand fly vectors from these sites was not observed. Hsp70 PCR and qPCR are useful techniques for determining infection status of vertebrate hosts. |
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2014 |
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2014 |
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2019-05-07T20:52:01Z |
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2019-05-07T20:52:01Z |
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Artículo de investigación |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Carrillo-Bonilla LM, Montoya A, Arbeláez N, Cadena H, Ramírez J, Robledo SM. Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster. Revista U.D.C.A Actualidad & Divulgación Científica. 2014; 17(2): 341-350 |
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0123-4226 |
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http://hdl.handle.net/10495/11005 |
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2619-2551 |
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Carrillo-Bonilla LM, Montoya A, Arbeláez N, Cadena H, Ramírez J, Robledo SM. Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster. Revista U.D.C.A Actualidad & Divulgación Científica. 2014; 17(2): 341-350 0123-4226 2619-2551 |
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http://hdl.handle.net/10495/11005 |
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eng |
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eng |
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Rev. Colomb. Cienc. Pecu. |
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350 |
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2 |
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341 |
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Revista UDCA actualidad & divulgación científica |
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Carrillo Bonilla, Lina MaríaMontoya, AndrésArbeláez, NataliaCadena, HoracioRamírez, JaimeRobledo Restrepo, Sara María2019-05-07T20:52:01Z2019-05-07T20:52:01Z2014Carrillo-Bonilla LM, Montoya A, Arbeláez N, Cadena H, Ramírez J, Robledo SM. Migration of Leishmania (Viannia) panamensis and its persistence in healthy skin of Hamster. Revista U.D.C.A Actualidad & Divulgación Científica. 2014; 17(2): 341-3500123-4226http://hdl.handle.net/10495/110052619-2551ABSTRACT: Leishmaniasis are endemic in >98 countries with 14 million people infected. Leishmania survives in mammal hosts even after treatment but the capacity to persist in healthy skin and infect sandflies from these sites is poorly understood. To demonstrate migration of Leishmania (V.) panamensis into healty skin, persistence in these sites during chronic stages of infection and infectivity of persistant parasites to Lutzomyia longipalpis 10 hamsters were inoculated intradermally in the hind footpad, with 2x107 L. (V.) panamensis promastigotes. Hamsters were sacrificed at 13 (acute phase) and 53 (chronic phase) weeks post-infection. Biopsies from lesion, healty skin and othert tissues were screened by Hsp70 polymerase chain reaction (PCR) and DNA polymerase qPCR. Transmission of parasites to sandflies was determined by xenodiagnosis. Positivity was observed in all samples of acute infection group except the contralateral ear and chronic infection group, although the percentage of positivity in chronic infection was lower than that for acute infection. Two animals of the acute infection group and one from the chronic infection group were infective to sand flies after feeding over the lesion site. No infections were found in sandflies fed from healthy skin. Dissemination and persistence of L. (V.) panamensis was reported for first time and persisntace is confirmed for a period of 53 weeks. However, parasite load in tissues different from the inoculation site was lower and transmission to the sand fly vectors from these sites was not observed. Hsp70 PCR and qPCR are useful techniques for determining infection status of vertebrate hosts.RESUMEN: Las leishmaniasis son endémicas en >98 países con 14 millones de infectados. Leishmania sobrevive en los mamíferos aún después del tratamiento pero se desconoce su capacidad de persistir en piel sana e infectar vectores. Para demostrar la migración de Leishmania (Viannia) panamensis a piel sana, su persistencia en estos sitios durante estados crónicos de infección y la infectividad de los parásitos persistentes para Lutzomyia longipalpis, se inocularon 10 hámsters intradérmicamente en pata con 2x107 promastigotes de L. (V.) panamensis. Los hámsters se sacrificaron a las 13 (fase aguda) y 53 (fase crónica) semanas post-infección. Biopsias de lesión, piel sana y otros tejidos se analizaron por reacción en cadena de la polimerasa (PCR) con el gen Hsp70 y qPCR con el gen DNA polymerase. La transmisión de los parásitos a Lu. longipalpis se determinó mediante xenodiagnóstico. Todas las muestras de fase aguda (excepto oreja contralateral) y todas las muestras de fase crónica fueron positivas, aunque la positividad en fase crónica fue menor que en fase aguda. Dos animales de fase aguda y un animal de fase crónica fueron infecciosos al vector, después de alimentarse sobre la lesión. No hubo ningún insecto positivo en los alimentados sobre piel sana. Se reporta por primera vez la diseminación y persistencia de L. (V) panamensis, y se confirma la persistencia en un periodo de 53 semanas. Sin embargo, la carga parasitaria en tejidos diferentes al sitio de inóculo fue baja y no se detectó su transmisión al vector. La PCR con Hsp70 y qPCR con DNA polymerase son técnicas útiles en la determinación del estatus infeccioso en los hospederos vertebrados.application/pdfengUniversidad de Ciencias aplicadas U.D.C.A.Medellín, Colombiahttps://creativecommons.org/licenses/by-nc/2.5/co/https://creativecommons.org/licenses/by-nc/4.0/Atribución-NoComercial 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Migración parasitariaParasite migrationXenodiagnosticoXenodiagnosisHsp70 PCRReal-time DNA polymeraseMigration of Leishmania (Viannia) panamensis and its persistence in healthy skin of HamsterMigración de Leishmania panamensis y su persistencia en piel sana de HamsterArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionRev. Colomb. Cienc. 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