Diversity of Toxigenic Bacillus cereus in Powder Milk

ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not...

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Autores:
Sánchez Chica, Jennifer
Correa Ochoa, Margarita María
Aceves Díez, Angel Emilio
Castañeda Sandoval, Laura Margarita
Tipo de recurso:
Article of investigation
Fecha de publicación:
2014
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/26453
Acceso en línea:
http://hdl.handle.net/10495/26453
https://revistas.unal.edu.co/index.php/refame/issue/view/5130
Palabra clave:
Enterotoxins
Enterotoxinas
Reacción en Cadena de la Polimerasa Multiplex
Multiplex Polymerase Chain Reaction
Microorganismos patógenos
Micro-organisms, pathogenic
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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repository_id_str
dc.title.spa.fl_str_mv Diversity of Toxigenic Bacillus cereus in Powder Milk
dc.title.translated.spa.fl_str_mv Diversidad de Bacillus cereus Toxigénicos en Leche en Polvo
title Diversity of Toxigenic Bacillus cereus in Powder Milk
spellingShingle Diversity of Toxigenic Bacillus cereus in Powder Milk
Enterotoxins
Enterotoxinas
Reacción en Cadena de la Polimerasa Multiplex
Multiplex Polymerase Chain Reaction
Microorganismos patógenos
Micro-organisms, pathogenic
title_short Diversity of Toxigenic Bacillus cereus in Powder Milk
title_full Diversity of Toxigenic Bacillus cereus in Powder Milk
title_fullStr Diversity of Toxigenic Bacillus cereus in Powder Milk
title_full_unstemmed Diversity of Toxigenic Bacillus cereus in Powder Milk
title_sort Diversity of Toxigenic Bacillus cereus in Powder Milk
dc.creator.fl_str_mv Sánchez Chica, Jennifer
Correa Ochoa, Margarita María
Aceves Díez, Angel Emilio
Castañeda Sandoval, Laura Margarita
dc.contributor.author.none.fl_str_mv Sánchez Chica, Jennifer
Correa Ochoa, Margarita María
Aceves Díez, Angel Emilio
Castañeda Sandoval, Laura Margarita
dc.contributor.researchgroup.spa.fl_str_mv BIOMICRO
Microbiología Molecular
dc.subject.decs.none.fl_str_mv Enterotoxins
Enterotoxinas
Reacción en Cadena de la Polimerasa Multiplex
Multiplex Polymerase Chain Reaction
topic Enterotoxins
Enterotoxinas
Reacción en Cadena de la Polimerasa Multiplex
Multiplex Polymerase Chain Reaction
Microorganismos patógenos
Micro-organisms, pathogenic
dc.subject.lemb.none.fl_str_mv Microorganismos patógenos
Micro-organisms, pathogenic
description ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not allow detecting the toxic potential and toxigenic diversity of the strains in food. Therefore, in this work, a multiplex PCR to direct detection of toxigenic B. cereus from powder milk samples collected in Medellín, Colombia, was applied. According to the pattern of toxic genes found in the samples, was possible to establish five different toxigenic consortia: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) and V (nheA, cesB). These results suggest a wide diversity of toxigenic B. cereus in the evaluated samples.
publishDate 2014
dc.date.issued.none.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2022-03-08T16:30:21Z
dc.date.available.none.fl_str_mv 2022-03-08T16:30:21Z
dc.type.spa.fl_str_mv Artículo de investigación
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dc.identifier.issn.none.fl_str_mv 0304-2847
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/26453
dc.identifier.eissn.none.fl_str_mv 2248-7026
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identifier_str_mv 0304-2847
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url http://hdl.handle.net/10495/26453
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dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Rev. Fac. Nac. Agron. Medellín
dc.relation.citationendpage.spa.fl_str_mv 853
dc.relation.citationissue.spa.fl_str_mv Suplemento 2
dc.relation.citationstartpage.spa.fl_str_mv 851
dc.relation.citationvolume.spa.fl_str_mv 67
dc.relation.ispartofjournal.spa.fl_str_mv Revista Facultad Nacional de Agronomía Medellín
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
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spelling Sánchez Chica, JenniferCorrea Ochoa, Margarita MaríaAceves Díez, Angel EmilioCastañeda Sandoval, Laura MargaritaBIOMICROMicrobiología Molecular2022-03-08T16:30:21Z2022-03-08T16:30:21Z20140304-2847http://hdl.handle.net/10495/264532248-7026https://revistas.unal.edu.co/index.php/refame/issue/view/5130ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not allow detecting the toxic potential and toxigenic diversity of the strains in food. Therefore, in this work, a multiplex PCR to direct detection of toxigenic B. cereus from powder milk samples collected in Medellín, Colombia, was applied. According to the pattern of toxic genes found in the samples, was possible to establish five different toxigenic consortia: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) and V (nheA, cesB). These results suggest a wide diversity of toxigenic B. cereus in the evaluated samples.RESUMEN : Bacillus cereus es una bacteria comúnmente aislada de alimentos, la cual produce toxinas como la hemolisina BL (HBL), enterotoxina no hemolítica (NHE), citotoxina K y toxina emética o cereúlida, que causan intoxicaciones alimentarias al hombre. La detección de B. cereus mediante pruebas tradicionales de cultivo en placa no permite determinar el potencial tóxico y diversidad toxigénica de las cepas; por lo cual, en este trabajo se aplicó una PCR múltiple que permitió la detección directa de B. cereus toxigénicos en muestras de leche en polvo colectadas en Medellín, Colombia. De acuerdo a los patrones de genes tóxicos encontrados en las muestras, fue posible establecer cinco consorcios toxigénicos diferentes: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) y V (nheA, cesB). Estos resultados sugieren una amplia diversidad de B. cereus toxigénicos en las muestras evaluadas.COL0111863COL00137463application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias AgrariasMedellín, Colombiahttp://creativecommons.org/licenses/by-nc-sa/2.5/co/https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Diversity of Toxigenic Bacillus cereus in Powder MilkDiversidad de Bacillus cereus Toxigénicos en Leche en PolvoArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionEnterotoxinsEnterotoxinasReacción en Cadena de la Polimerasa MultiplexMultiplex Polymerase Chain ReactionMicroorganismos patógenosMicro-organisms, pathogenicRev. Fac. Nac. Agron. Medellín853Suplemento 285167Revista Facultad Nacional de Agronomía MedellínPublicationORIGINALSanchezJennifer_2014_DiversityToxigenicBacillus.pdfSanchezJennifer_2014_DiversityToxigenicBacillus.pdfArtículo de investigaciónapplication/pdf193525https://bibliotecadigital.udea.edu.co/bitstreams/b54b6c81-382e-44fe-9f02-3415f6a72479/downloada04dcf77b241ec2fe56c7ec6b276cd1cMD51trueAnonymousREADCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstreams/5fe32ad0-74f3-4e0b-80fd-fb47dd3fd1df/downloade2060682c9c70d4d30c83c51448f4eedMD52falseAnonymousREADLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstreams/2cbb0cc0-b11a-4f62-b160-1d7c351e76dd/download8a4605be74aa9ea9d79846c1fba20a33MD53falseAnonymousREADTEXTSanchezJennifer_2014_DiversityToxigenicBacillus.pdf.txtSanchezJennifer_2014_DiversityToxigenicBacillus.pdf.txtExtracted texttext/plain13905https://bibliotecadigital.udea.edu.co/bitstreams/a319b0e5-8955-4e44-80b0-9cb25a4a2660/downloadf7abe25a58f396b6f02ccd98b33d2c5cMD56falseAnonymousREADTHUMBNAILSanchezJennifer_2014_DiversityToxigenicBacillus.pdf.jpgSanchezJennifer_2014_DiversityToxigenicBacillus.pdf.jpgGenerated Thumbnailimage/jpeg15365https://bibliotecadigital.udea.edu.co/bitstreams/6ddb6042-9336-48f0-8bff-8242c1b5f0e2/download863a983f98e0d90b7854c67eb9619c79MD57falseAnonymousREAD10495/26453oai:bibliotecadigital.udea.edu.co:10495/264532025-03-26 18:02:17.796http://creativecommons.org/licenses/by-nc-sa/2.5/co/open.accesshttps://bibliotecadigital.udea.edu.coRepositorio Institucional de la Universidad de Antioquiaaplicacionbibliotecadigitalbiblioteca@udea.edu.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