Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis
ABSTRACT: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora...
- Autores:
-
Aguillón Osma, Johanny
Maldonado Celis, María Elena
Loango Chamorro, Nelsy
Arango Varela, Sandra Sulay
Landázuri, Patricia
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/11380
- Acceso en línea:
- http://hdl.handle.net/10495/11380
- Palabra clave:
- Passiflora edulis
Antioxidant
Antiproliferative
Cytotoxic
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-sa/4.0/
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Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| dc.title.translated.spa.fl_str_mv |
Actividad antioxidante y antiproliferativa de extractos etanólico y acuoso de las hojas y el jugo del fruto de Passiflora edulis |
| title |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| spellingShingle |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis Passiflora edulis Antioxidant Antiproliferative Cytotoxic |
| title_short |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_full |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_fullStr |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_full_unstemmed |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_sort |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| dc.creator.fl_str_mv |
Aguillón Osma, Johanny Maldonado Celis, María Elena Loango Chamorro, Nelsy Arango Varela, Sandra Sulay Landázuri, Patricia |
| dc.contributor.author.none.fl_str_mv |
Aguillón Osma, Johanny Maldonado Celis, María Elena Loango Chamorro, Nelsy Arango Varela, Sandra Sulay Landázuri, Patricia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Impacto de Componentes Alimentarios en la Salud |
| dc.subject.none.fl_str_mv |
Passiflora edulis Antioxidant Antiproliferative Cytotoxic |
| topic |
Passiflora edulis Antioxidant Antiproliferative Cytotoxic |
| description |
ABSTRACT: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora edulis. Materials and methods: A preliminary phytochemical screening was performed; antioxidant activity was evaluated through DPPH assay, the scavenging activity for hydroxyl radical, antihemolytic activity and total phenolic content; cytotoxic and antiproliferative activities were evaluated by MTT and sulforhodamine B assays respectively in colon adenocarcinoma SW480 cells and their metastatic-derived SW620 cells. Results: Phytochemical analyses revealed presence of tannins, flavonoids and cardiotonic glycosides. Ethanolic extract from leaves showed the best antioxidant activity (EC50 = 0.096 mg/ml) in the DPPH assay and the juice (EC50 = 0.022 mg/ml) for the Hydroxyl free radical-scavenging activity. All extracts inhibited more than 98% the hemolysis induced by H2O2. The aqueous extract from leaves showed the highest cytotoxic activity against SW480 and SW620 cells. Conclusions: Findings from this study suggest that P. edulis is a potential source of phytochemical compounds with antioxidant and antiproliferative properties. |
| publishDate |
2013 |
| dc.date.issued.none.fl_str_mv |
2013 |
| dc.date.accessioned.none.fl_str_mv |
2019-07-09T14:44:05Z |
| dc.date.available.none.fl_str_mv |
2019-07-09T14:44:05Z |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Aguillón J, Maldonado ME, Loango N, Arango SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspect. Nutr. Humana.. 2013; 15:13-25. |
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0124-4108 |
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http://hdl.handle.net/10495/11380 |
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2248-454X |
| identifier_str_mv |
Aguillón J, Maldonado ME, Loango N, Arango SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspect. Nutr. Humana.. 2013; 15:13-25. 0124-4108 2248-454X |
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http://hdl.handle.net/10495/11380 |
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spa |
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spa |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Perspect. Nutr. Humana. |
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25 |
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1 |
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13 |
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15 |
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Perspectivas en Nutrición Humana |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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Universidad de Antioquia, Escuela de Nutrición y Dietética |
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Medellín, Colombia |
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Universidad de Antioquia |
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Aguillón Osma, JohannyMaldonado Celis, María ElenaLoango Chamorro, NelsyArango Varela, Sandra SulayLandázuri, PatriciaImpacto de Componentes Alimentarios en la Salud2019-07-09T14:44:05Z2019-07-09T14:44:05Z2013Aguillón J, Maldonado ME, Loango N, Arango SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspect. Nutr. Humana.. 2013; 15:13-25.0124-4108http://hdl.handle.net/10495/113802248-454XABSTRACT: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora edulis. Materials and methods: A preliminary phytochemical screening was performed; antioxidant activity was evaluated through DPPH assay, the scavenging activity for hydroxyl radical, antihemolytic activity and total phenolic content; cytotoxic and antiproliferative activities were evaluated by MTT and sulforhodamine B assays respectively in colon adenocarcinoma SW480 cells and their metastatic-derived SW620 cells. Results: Phytochemical analyses revealed presence of tannins, flavonoids and cardiotonic glycosides. Ethanolic extract from leaves showed the best antioxidant activity (EC50 = 0.096 mg/ml) in the DPPH assay and the juice (EC50 = 0.022 mg/ml) for the Hydroxyl free radical-scavenging activity. All extracts inhibited more than 98% the hemolysis induced by H2O2. The aqueous extract from leaves showed the highest cytotoxic activity against SW480 and SW620 cells. Conclusions: Findings from this study suggest that P. edulis is a potential source of phytochemical compounds with antioxidant and antiproliferative properties.RESUMEN: Extractos de varios árboles frutales se han utilizado en diferentes aplicaciones terapéuticas para prevenir el estrés oxidativo asociado a enfermedades crónicas. Objetivo: investigar la actividad antioxidante y antiproliferativa de extractos etanólico y acuosos de las hojas y del fruto de Passiflora edulis. Materiales y métodos: se realizó marcha fitoquímica preliminar, la actividad antioxidante se evaluó por DPPH, actividad de remoción de radicales hidroxilo, actividad antihemolítica y contenido fenólico total. En células de adenocarcinoma de colon SW480 y sus derivadas metastásicas SW620 se evaluó la actividad citotóxica y antiproliferativa por el método de MTT y sulforodamina B respectivamente. Resultados: el análisis fitoquímico reveló la presencia de taninos, flavonoides y glicósidos cardiotónicos. El extracto etanólico de las hojas mostró la mayor actividad antioxidante (EC50 = 0,096 mg/ml) por DPPH y el jugo (EC50 = 0,022 mg/ml) para remover el radical hidroxilo. Todos los extractos inhibieron más del 98% la hemólisis inducida por H2O2. El extracto acuoso de las hojas mostró la mayor actividad citotóxica y antiproliferativa contra células SW480 y SW620. Conclusiones: los hallazgos de este estudio sugieren que P. edulis es una fuente potencial de compuestos fitoquímicos con propiedades antioxidante y antiproliferativa.application/pdfspaUniversidad de Antioquia, Escuela de Nutrición y DietéticaMedellín, Colombiahttps://creativecommons.org/licenses/by-nc-sa/4.0/https://creativecommons.org/licenses/by-nc-sa/2.5/co/Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Passiflora edulisAntioxidantAntiproliferativeCytotoxicAntioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulisActividad antioxidante y antiproliferativa de extractos etanólico y acuoso de las hojas y el jugo del fruto de Passiflora edulisArtículo de investigaciónhttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPerspect. Nutr. 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